Effects of Immobilizations of rhBMP-2 and/or rhPDGF-BB on Titanium Implant Surfaces on Osseointegration and Bone Regeneration
Source abstract
Abstract
The aim of this study was to examine the effects of immobilizing rhPDGF-BB plus rhBMP-2 on heparinized-Ti implants on in vivo osseointegration and vertical bone regeneration at alveolar ridges. Successful immobilizations of rhPDGF-BB and/or rhBMP-2 onto heparinized-Ti (Hepa/Ti) were confirmed by in vitro analysis, and both growth factors were found to be sustained release. To evaluate bone regeneration, rhPDGF-BB, and/or rhBMP-2-immobilized Hepa/Ti implants were inserted into beagle dogs; implant stability quotients (ISQ), bone mineral densities, bone volumes, osseointegration, and bone formation were assessed by micro CT and histometrically. In vivo study showed that the osseointegration and bone formation were greater in the rhPDGF-BB/rhBMP-2-immobilized Hepa/Ti group than in the rhPDGF-BB-immobilized Hepa/Ti group. The rhPDGF-BB/rhBMP-2 immobilized Hepa/Ti group also showed better implant stability and greater bone volume around defect areas and intra-thread bone density (ITBD) than the rhBMP-2-immobilized Hepa/Ti group. However, no significant differences were observed between these two groups. Through these results, we conclude rhBMP-2 immobilized, heparin-grafted implants appear to offer a suitable delivery system that enhances new bone formation in defect areas around implants. However, we failed to observe the synergetic effects for the rhBMP-2 and rhPDGF-BB combination.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental Implant Techniques and Outcomes · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Prosthodontics, Dental Research Institute, Institute of Translational Dental Sciences, BK21 PLUS Project, School of Dentistry, Pusan National University, 49 Pusan University-Ro, Yangsan-Si 50612, Gyeongsangnam-Do, Korea
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea
References (56)
Show source-provided references
1. Alveolar ridge augmentation with titanium mesh. A retrospective clinical study10.2174/1874210601408010148 ↗
2. Effect of diabetes and metabolic control on de novo bone formation following guided bone regeneration10.1111/j.1600-0501.2009.01805.x ↗
3. Clinical study on the primary stability of two dental implant systems with resonance frequency analysis10.1007/s00784-007-0115-2 ↗
4. Bone morphogenetic proteins10.1080/08977190412331279890 ↗
5. Bone formation at rhBMP-2-coated titanium implants in the rat ectopic model10.1111/j.1600-051X.2007.01064.x ↗
6. Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration10.1002/term.1668 ↗
7. Bone morphogenetic proteins in tissue engineering: The road from laboratory to clinic, part II (BMP delivery)10.1002/term.74 ↗
8. Bone morphogenetic proteins in tissue engineering: The road from the laboratory to the clinic, part I (basic concepts)10.1002/term.63 ↗
9. Bone apposition to titanium implants biocoated with recombinant human bone morphogenetic protein-2 (rhBMP-2). A pilot study in dogs10.1007/s00784-006-0049-0 ↗
10. Bone formation at recombinant human bone morphogenetic protein-2-coated titanium implants in the posterior mandible (Type II bone) in dogs10.1111/j.1600-051X.2008.01318.x ↗
11. Titanium implants and BMP-7 in bone: An experimental model in the rabbit10.1023/A:1022884724059 ↗
12. Effect of immobilized bone morphogenic protein 2 coating of titanium implants on peri-implant bone formation10.1111/j.1600-0501.2005.01143.x ↗
13. The effect on bone regeneration of a liposomal vector to deliver BMP-2 gene to bone grafts in peri-implant bone defects10.1016/j.biomaterials.2007.02.009 ↗
14. Evaluation of osseointegration of dental implants coated with collagen, chondroitin sulphate and BMP-4: An animal study10.1016/j.ijom.2007.05.024 ↗
15. Effect of PDGF, IGF-1 and PRP on the implant osseointegration. A histological and immunohistochemical study in rabbits
16. Evaluation of Different Combinations of Biphasic Calcium Phosphate and Growth Factors for Bone Formation in Calvarial Defects in a Rabbit Model10.11607/prd.2633 ↗
17. A comparative evaluation of rhPDGF-BB + β-TCP and subepithelial connective tissue graft for the treatment of multiple gingival recession defects in humans10.11607/prd.1726 ↗
18. Human histologic and clinical evaluation of recombinant human platelet-derived growth factor and beta-tricalcium phosphate for the treatment of periodontal intraosseous defects
19. Randomized controlled clinical study of rhPDGF-BB + β-TCP versus HA + β-TCP for the treatment of infrabony periodontal defects: Clinical and radiographic results
20. Efficacy of modified minimally invasive surgical technique in the treatment of human intrabony defects with or without use of rhPDGF-BB gel: A randomized controlled trial10.1111/jcpe.12030 ↗
21. Mechanisms of bone repair and regeneration10.1016/j.molmed.2009.07.002 ↗
22. Misch, C.M., Nevins, M.L., and Boch, J.A. (2015). Recombinant Human Bone Morphogenetic Protein-2 or Recombinant Human Platelet-Derived Growth Factor BB in Extraction Site Preservation and Bone Augmentation. Minimally Invasive Dental Implant Surgery, Wiley-Blackwell.10.1002/9781119421405.ch7 ↗
23. Release of growth factors and the effect of age, sex, and severity of injury after long bone fracture10.3109/17453674.2013.765624 ↗
24. PDGF in bone formation and regeneration: New insights into a novel mechanism involving MSCs10.1002/jor.21462 ↗
25. Relative binding and biochemical effects of heterodimeric and homodimeric isoforms of platelet-derived growth factor in osteoblast-enriched cultures from fetal rat bone10.1002/jcp.1041470306 ↗
26. Accelerated fracture healing in the geriatric, osteoporotic rat with recombinant human platelet-derived growth factor-bb and an injectable beta-tricalcium phosphate/collagen matrix10.1002/jor.20453 ↗
27. The effect of systemically administered PDGF-BB on the rodent skeleton10.1002/jbmr.5650110213 ↗
28. Heparin-binding domain of fibrin (ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix10.1073/pnas.1221602110 ↗
29. Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres10.1002/jbm.a.10542 ↗
30. Bone formation on apatite-coated titanium with incorporated BMP-2/heparin in vivo10.1016/j.tripleo.2009.06.039 ↗
31. Effect of immobilization of the recombinant human bone morphogenetic protein 2 (rhBMP-2) on anodized implants coated with heparin for improving alveolar ridge augmentation in beagle dogs: Radiographic observations10.4047/jkap.2013.51.4.307 ↗
32. Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation10.4047/jap.2011.3.3.152 ↗
33. Effect of PDGF-BB and beta-tricalcium phosphate (β-TCP) on bone formation around dental implants: A pilot study in sheep10.1111/j.1600-0501.2011.02345.x ↗
34. Alveolar ridge augmentation using anodized implants coated with Escherichia coli–derived recombinant human bone morphogenetic protein 210.1016/j.tripleo.2010.09.063 ↗
35. Effects of anodized implants coated with Escherichia coli-derived rhBMP-2 in beagle dogs10.1016/j.ijom.2012.04.005 ↗
36. Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin10.1016/j.carbpol.2014.08.005 ↗
37. A randomized-controlled clinical trial evaluating clinical and radiological outcomes after 3 and 5 years of dental implants placed in bone regenerated by means of GBR techniques with or without the addition of BMP-210.1111/j.1600-0501.2008.01648.x ↗
38. Effect of rhBMP-2 on guided bone regeneration in humans10.1034/j.1600-0501.2003.00921.x ↗
39. De novo bone induction by recombinant human bone morphogenetic protein-2 (rhBMP-2) in maxillary sinus floor augmentation10.1016/j.joms.2005.08.018 ↗
40. Heparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-210.1074/jbc.M511039200 ↗
41. Heparin-functionalized chitosanealginate scaffolds for controlled release of growth factor10.1016/j.ijpharm.2009.04.048 ↗
42. The effect of crosslinking heparin to demineralized bone matrix on mechanical strength and specific binding to human bone morphogenetic protein-210.1016/j.biomaterials.2007.11.032 ↗
43. Surface treatments of titanium dental implants for rapid osseointegration10.1016/j.dental.2006.06.025 ↗
44. Inorganic material surfaces made bioactive by immobilizing growth factors for hard tissue engineering10.1039/c3ra23313h ↗
45. The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammationand osteoblast function10.1016/j.biomaterials.2010.09.008 ↗
46. Attenuation of the in vivo toxicity of biomaterials by polydopamine surface modification10.2217/nnm.11.76 ↗
47. Mussel-inspired surface chemistry for multifunctional coatings10.1126/science.1147241 ↗
48. Biomimetic anchor for surface-initiated polymerization from metal substrates10.1021/ja0532638 ↗
49. Gentamicin and bone morphogenic protein-2 (BMP-2)-delivering heparinized-titanium implant with enhanced antibacterial activity and osteointegration10.1016/j.bone.2012.01.007 ↗
50. Differential growth factor control of bone formation through osteoprogenitor differentiation10.1016/j.bone.2003.11.014 ↗
51. The synergetic bone-forming effects of combinations of growth factors expressed by adenovirus vectors on chitosan/collagen scaffolds10.1016/j.jconrel.2009.02.016 ↗
52. Effects of rhBMP-2 on sandblasted and acid etched titanium implant surfaces on bone regeneration and osseointegration: Spilt-mouth designed pilot study10.1155/2015/812949 ↗
53. Surface functionalization of titanium with carboxymethyl chitosan and immobilized bone morphogenetic protein-2 for enhanced osseointegration10.1021/bm900203w ↗
54. Isolation of monoclonal antibodies recognizing rat bone-associated molecules in vitro and in vivo10.1177/40.9.1506671 ↗
55. Functionalization of multilayered DNA-coatings with bone morphogenetic protein 210.1016/j.jconrel.2006.03.016 ↗
56. Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation10.1002/jbm.a.35334 ↗