Antimicrobial Activity and the Effect of Green Tea Experimental Gels on Teeth Surfaces
Source abstract
Abstract
Among esthetic procedures, teeth whitening is a common and often used treatment for patients who seek good teeth appearance. We developed an experimental green tea extract and an experimental green tea gel for enamel restoring treatment after bleaching. We also tested the antibacterial and antifungal effect of the experimental extract against specific endodontic and cariogenic microorganisms. The green tea extract antibacterial action was determined by the disk-diffusion method using Peptostreptococcus anaerobius (ATCC27337), Corynebacterium xerosis (ATCC 373), Streptococcus mutans (ATCC 25175) and Candida albicans (ATCC 10231) strains. Enamel microstructure was investigated by SEM analysis, and surface details were revealed by AFM. The inhibition zones around the wells showed evident antimicrobial activity of the experimental extract. In the presence of Candida albicans (ATCC 10231), the extract showed no antifungal activity. The enamel’s surface roughness and hydroxyapatite prism aspects were the parameters followed throughout the study. The experimental green tea extract is efficient against some microorganisms commonly found in the oral cavity. However, the studied extract had no antifungal effect. The results show that after bleaching with the experimental gel, we obtained the best surface parameters, similar to healthy enamel.
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Research context
OpenAlex topics: Dental Erosion and Treatment · Oral microbiology and periodontitis research · Endodontics and Root Canal Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Odontology and Oral Pathology, Faculty of Dental Medicine, University of Medicine and Pharmacy “Iuliu Hațieganu”, 33 Motilor Street, 400001 Cluj-Napoca, Romania
Department of Functional Sciences, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 3 Clinicilor Street, 400006 Cluj-Napoca, Romania
Department of Medical Informatics and Biostatistics, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu’, 6 Pasteur Louis Street, 400349 Cluj-Napoca, Romania
Physics and Chemistry Department, Technical University of Cluj-Napoca, 28 Memorandumului Street, 400114 Cluj-Napoca, Romania
Faculty of Chemistry and Chemical Engineering, Babes Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, Romania
University of Medicine and Pharmacy Carol Davila Bucharest, 050474 Bucharest, Romania
Orthopedics and Traumatology Department, Bucharest Emergency University Hospital, 050098 Bucharest, Romania
Institute of Chemistry Raluca Ripan, Babes-Bolyai University, 30 Fantanele Str, 400294 Cluj-Napoca, Romania
Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu Hatieganu”, 6 Pasteur Louis Street, 400349, Cluj-Napoca, Romania
References (55)
Show source-provided references
1. Lazarescu, F. (2013). Incursiune în estetica dentară, SSER Publishing House.
2. Antioxidant effects of green tea10.1002/mnfr.201000641 ↗
3. Green tea extract: Chemistry, antioxidant properties and food applications–A review10.1016/j.jff.2013.08.011 ↗
4. Impact of the green tea ingredient epigallocatechin gallate and a short pentapeptide (Ile-Ile-Ala-Glu-Lys) on the structural organization of mixed micelles and the related uptake cholesterol10.1016/j.bbagen.2018.06.005 ↗
5. Regulation of matrix mettaloproteinase secretion by green tea catechins in a three-dimendional co-culture model of macrophages and gingival fibroblasts10.1016/j.archoralbio.2016.10.035 ↗
6. The in vitro antibacterial afficacy of Persian green tea extract as an intracanal irrigant on Enterococcus faecalis biofilm
7. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment10.1016/j.joen.2005.10.049 ↗
8. Microbial analysis of canals of root filled teeth with periapical lesions using polymerase chain reaction10.1016/j.joen.2008.01.016 ↗
9. Critical review on eliminating endodontic dental infections using herbal products10.1080/19390211.2016.1207004 ↗
10. Evaluation of home bleaching gel modified by different thickeners on the physical properties of enamel: An in situ study10.4103/ejd.ejd_352_17 ↗
11. Influence of time intervals between bleaching procedures on enamel microhardness and surface roughness10.2174/1874210601812010555 ↗
12. González-Ochoa, J.G. (2002). Histological changes to dental pulp after vital bleaching with 10% carbamide peroxide. [Master’s Thesis, Indiana University School of Dentistry].
13. Effect of home-use and in-office bleaching agents containing hydrogen peroxide associated with amorphous calcium phosphate on enamel microhardness and surface roughness10.1111/j.1708-8240.2010.00394.x ↗
14. The safety of home bleaching techniques
15. Preventive effect of different toothpastes on enamel erosion: AFM and SEM studies10.1002/sca.21132 ↗
16. Dental enamel roughness with different acid etching times: Atomic force microscopy study
17. Characterization of Peptostreptococcus species in skin infections10.1177/147323000002800305 ↗
18. Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification10.1128/IAI.67.10.5170-5175.1999 ↗
19. Bacteriology of moderate (chronic) periodontitis in mature adult humans10.1128/iai.42.2.510-515.1983 ↗
20. Real time PCR quantifi-cation of 9 periodontal pathogens in saliva samples from periodontally healthy Koreean young adults10.5051/jpis.2018.48.4.261 ↗
21. Hplc analysis, antimicrobial and antifungal activity of an experimental plant based gel, for endodontic usage
22. Root canal microbiota of teeth with chronic apical periodontitis10.1128/JCM.00431-08 ↗
23. Molecular and cultural analysis of the microflora associated with endodontic infections10.1177/0810761 ↗
24. Associations between microbial species in dental root canal infections10.1111/j.1399-302X.1992.tb00584.x ↗
25. A comparative evaluation of antibacterial effectiveness of sodium hypochlorite, Curcuma longa, and Camellia sinensis as irrigating solutions on isolated anaerobic bacteria from infected primary teeth10.4103/0970-4388.180447 ↗
26. Infections caused by nondiphtheria corynebacteria10.1093/clinids/4.6.1220 ↗
27. Identification of bacteria in acute endodontic infections and their antimicrobial susceptibility10.1067/moe.2002.129535 ↗
28. Inhibitory effect of natural herbal extracts on systemic bacteria
29. Sakagami, H., Watanabe, T., Hoshino, T., Suda, N., Mori, K., Yasui, T., Yamauchi, N., Kashiwagi, H., Gomi, T., and Oizumi, T. (2019). Recent progress of basic studies of natural products and their dental application. Medicines, 6.10.3390/medicines6010004 ↗
30. Natural antimicrobials and oral microorganisms:a systematic review on herbal interventions for eradication of multispecies oral biofilms
31. Oolong tea polyphenols inhibit experimental dental caries in SPF rats infected with mutans streptococci10.1159/000261529 ↗
32. Inhibitory effect of oolong tea polyphenols of glycosyltransferases of mutans streptococci10.1128/aem.59.4.968-973.1993 ↗
33. Anticaries effects of polyphenolic compounds from Japanese green tea10.1159/000261407 ↗
34. Antibacterial activity of Camellia sinensis extracts against dental caries10.1007/BF02975300 ↗
35. Antimicrobial effect of malaysian green tea leaves (camellia sinensis) on the skin microbiota
36. Antimicrobial activity of the green tea polyphenol (−)-epigallocatechin-3-gallate (EGCG) against clinical isolates of Stenotrophomonas maltophilia10.1016/j.ijantimicag.2010.03.025 ↗
37. Fabrication and characterization of TiO2 coated cone shaped nano-fiber pH sensor10.1016/j.optcom.2016.11.021 ↗
38. Polyaniline-coated tilted fiber Bragg gratings for pH sensing10.1016/j.snb.2017.07.167 ↗
39. Hydrogen peroxide whitens teeth by oxidizing the organic structure10.1016/j.jdent.2012.08.008 ↗
40. Tooth bleaching with low-temperature plasma lowers surface roughness and streptococcus mutans adhesion10.1111/iej.12860 ↗
41. In situ and in vitro effects of bleaching with carbamide peroxide on human enamel
42. Características clínicas e patológicas da cárie dentária
43. Effect of 30% hydrogen peroxide on mineral chemical composition and surface morphology of bovine enamel10.1007/s10266-014-0189-7 ↗
44. Comparative evaluation of two antioxidants on reversing the immediate bond strength of bleached enamel: In vitro study
45. Comparative analysis of antimicrobial activity of leaf extracts from fresh green tea, commercial green tea and black tea on pathogens
46. Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans10.1093/jac/dkh046 ↗
47. Preliminary studies on denture plaque microbiologyand acidogenicity10.3109/08910608809140179 ↗
48. Nutritional factors and oral candidosis10.1111/j.1600-0714.1986.tb00578.x ↗
49. Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo10.1128/IAI.00087-14 ↗
50. Green tea catechin inhibits lipopolysaccharide-induced bone resorption in vivo10.1111/j.1600-0765.2008.01198.x ↗
51. Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea10.1111/bph.12009 ↗
52. Enamel surface changes after exposure to bleaching gels containing carbamide peroxide or hydrogen peroxide10.2341/15-010-L ↗
53. Effect of three nanobiomaterials on the surface roughness of bleached enamel10.4103/0976-237X.169853 ↗
54. Mazilu, A., Sarosi, C., Moldovan, M., Filip, M., Prodan, D., Antoniac, A., Prejmerean, C., Silaghi-Dumitrescu, L., Popescu, V., and Raiciu, A.D. (2019). Preparation and characterization of natural bleaching gels used in cosmetic dentistry. Materials, 12.10.3390/ma12132106 ↗
55. Effect of fluoride treatments on bleached enamel microhardness and surface morphology