Dual Synergistic Effects of MgO-GO Fillers on Degradation Behavior, Biocompatibility and Antibacterial Activities of Chitosan Coated Mg Alloy
Source abstract
Abstract
The aim of this work was to establish and characterize chitosan/graphene oxide- magnesium oxide (CS/GO-MgO) nanocomposite coatings on biodegradable magnesium-zinc-cerium (Mg-Zn-Ce) alloy. In comparison to that of pure CS coatings, all composite coatings encapsulating GO-MgO had better adhesion strength to the Mg-Zn-Ce alloy substrate. The result depicted that the co-encapsulation of GO-MgO into the CS layer leads to diminish of contact angle value and hence escalates the hydrophilic characteristic of coated Mg alloy. The electrochemical test demonstrated that the CS/GO-MgO coatings significantly increased the corrosion resistance because of the synergistic effect of the GO and MgO inside the CS coating. The composite coating escalated cell viability and cell differentiation, according to cytocompatibility tests due to the presence of GO and MgO within the CS. The inclusion of GO-MgO in CS film, on the other hand, accelerates the formation of hydroxyapatite (HA) during 14 days immersion in SBF. Immersion results, including weight loss and hydrogen evolution tests, presented that CS/GO-MgO coating enables a considerably reduced degradation rate of Mg-Zn-Ce alloy when compared to the bare alloy. In terms of antibacterial-inhibition properties, the GO-MgO/CS coatings on Mg substrates showed antibacterial activity against Escherichia coli (E. coli), with a large inhibition area around the specimens, particularly for the coating containing a higher concentration of GO-MgO. Bacterial growth was not inhibited by the bare Mg alloy samples. The CS/GO-MgO composite coating is regarded as a great film to enhance the corrosion resistance, bioactivity, and antibacterial performance of Mg alloy implants.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Magnesium Alloys: Properties and Applications · Bone Tissue Engineering Materials · MXene and MAX Phase Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Department of Materials and Metallurgical Engineering, Amirkabir University of Technology, Tehran, Iran
Iranian National Standards Organization, Tehran, Iran
Advanced Membrane Technology Research Center (AMTEC), University Teknologi Malaysia, Johor Bahru 81310, Johor, Malaysia
Faculty of Engineering, University Teknologi Malaysia, Johor Bahru 81310, Johor, Malaysia
Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore
Department of Chemical and Materials Engineering, University of Nevada, Reno, NV 89501, USA
Department of Mechanical and Industrial Engineering, Norwegian University of Science and Technology, 7491 Trondheim, Norway
References (52)
Show source-provided references
1. A self-healing corrosion protection coating with graphene oxide carrying 8-hydroxyquinoline doped in layered double hydroxide on a micro-arc oxidation coating10.1016/j.corsci.2021.109941 ↗
2. A new strategy for developing chitosan conversion coating on magnesium substrates for orthopedic implants10.1016/j.apsusc.2018.10.002 ↗
3. New approach for improving anticorrosion and biocompatibility of magnesium alloys via polydopamine intermediate layer-induced hydroxyapatite coating10.1016/j.surfin.2020.100501 ↗
4. In vitro study of polycaprolactone/bioactive glass composite coatings on corrosion and bioactivity of pure Mg10.1016/j.apsusc.2015.07.053 ↗
5. In vitro degradation behavior and cytocompatibility of biodegradable AZ31 alloy with PEO/HT composite coating10.1016/j.colsurfb.2015.02.011 ↗
6. The chitosan coating and processing effect on the physiological corrosion behaviour of porous magnesium monoliths10.1016/j.porgcoat.2016.05.019 ↗
7. Immobilization of bioactive complex on the surface of magnesium alloy stent material to simultaneously improve anticorrosion, hemocompatibility and antibacterial activities10.1016/j.colsurfb.2020.111541 ↗
8. Antibacterial activity of chitosan and its derivatives and their interaction mechanism with bacteria: Current state and perspectives10.1016/j.eurpolymj.2020.109984 ↗
9. Surface modification of Ti-6Al-4V alloy for osseointegration by alkaline treatment and chitosan-matrix glass-reinforced nanocomposite coating10.1016/j.carbpol.2018.10.082 ↗
10. Electrochemical characterization of Electrophoretically deposited Hydroxyapatite/Chitosan/Graphene Oxide composite coating on Mg substrate10.1016/j.surfin.2021.101290 ↗
11. Improved antibacterial properties of an Mg-Zn-Ca alloy coated with chitosan nanofibers incorporating silver sulfadiazine multiwall carbon nanotubes for bone implants10.1002/pat.4563 ↗
12. Ternary hydroxyapatite/chitosan/graphene oxide composite coating on AZ91D magnesium alloy by electrophoretic deposition10.1016/j.ceramint.2021.06.120 ↗
13. Electrophoretic deposition of chitosan-based composite coatings for biomedical applications: A review10.1016/j.pmatsci.2019.01.001 ↗
14. A short review on mechanical properties of graphene reinforced metal matrix composites10.1016/j.jmrt.2020.01.077 ↗
15. Electrophoretic deposition of chitosan reinforced graphene oxide-hydroxyapatite on the anodized titanium to improve biological and electrochemical characteristics10.1016/j.msec.2018.12.136 ↗
16. Effect of mechanical alloying on the phase evolution, microstructure and bio-corrosion properties of a Mg/HA/TiO2/MgO nanocomposite10.1016/j.ceramint.2014.08.039 ↗
17. Interfacial strengthening by reduced graphene oxide coated with MgO in biodegradable Mg composites10.1016/j.matdes.2020.108612 ↗
18. nMgO-incorporated PLLA bone scaffolds: Enhanced crystallinity and neutralized acidic products10.1016/j.matdes.2019.107801 ↗
19. The electrochemical behavior, antifungal and cytotoxic activities of phytofabricated MgO nanoparticles using Withania somnifera leaf extract10.1016/j.jsamd.2019.01.003 ↗
20. Nanofibrillated chitosan coated highly ordered titania nanotubes array/graphene nanocomposite with improved biological characters10.1016/j.carbpol.2020.117465 ↗
21. Carbon nanotubes and graphene as nanoreinforcements in metallic biomaterials: A review10.1002/adbi.201800212 ↗
22. Shahin, M., Wen, C., Munir, K., and Li, Y. (2021). Mechanical and corrosion properties of graphene nanoplatelet–reinforced Mg–Zr and Mg–Zr–Zn matrix nanocomposites for biomedical applications. J. Magnes. Alloy.10.1016/j.jallcom.2021.161379 ↗
23. Bi-layer nano-TiO2/FHA composite coatings on Mg–Zn–Ce alloy prepared by combined physical vapour deposition and electrochemical deposition methods10.1016/j.vacuum.2014.08.013 ↗
24. High-efficient Synthesis of Graphene Oxide Based on Improved Hummers Method10.1038/srep36143 ↗
25. Facile preparation of MgO/graphene oxide nanocomposite for efficient removal of aqueous Congo red: Adsorption performance and interaction mechanism10.1007/s11164-020-04310-9 ↗
26. Co-incorporation of graphene oxide/silver nanoparticle into poly-L-lactic acid fibrous: A route toward the development of cytocompatible and antibacterial coating layer on magnesium implants10.1016/j.msec.2020.110812 ↗
27. Simple synthesis of graphene nanocomposites MgO–rGO and Fe2O3–rGO for multifunctional applications10.1007/s00339-018-1748-5 ↗
28. Highly corrosion protection properties of plasma electrolytic oxidized titanium using rGO nanosheets10.1016/j.apsusc.2019.05.026 ↗
29. Homogeneous Chitosan/Graphene Oxide Nanocomposite Hydrogel-Based Actuator Driven by Efficient Photothermally Induced Water Gradients10.1021/acsanm.9b02338 ↗
30. In-situ grown MgO-ZnO ceramic coating with high thermal emittance on Mg alloy by plasma electrolytic oxidation10.1016/j.actaastro.2017.03.021 ↗
31. Liu, L., Peng, F., Zhang, D., Li, M., Huang, J., and Liu, X. (2021). A tightly bonded reduced graphene oxide coating on magnesium alloy with photothermal effect for tumor therapy. J. Magnes. Alloy.10.1016/j.jma.2021.03.003 ↗
32. Electrophoretic deposition of gentamicin-loaded bioactive glass/chitosan composite coatings for orthopaedic implants10.1021/am5014166 ↗
33. Mechanical properties and biomedical applications of a nanotube hydroxyapatite-reduced graphene oxide composite10.1016/j.carbon.2013.11.054 ↗
34. A multifunctional polymeric coating incorporating lawsone with corrosion resistance and antibacterial activity for biomedical Mg alloys10.1016/j.porgcoat.2021.106157 ↗
35. Evaluation of corrosion resistance of magnesium alloy ZK10 coated with hybrid organic–inorganic film including containers10.1016/j.corsci.2012.08.052 ↗
36. Silk fibroin film-coated MgZnCa alloy with enhanced in vitro and in vivo performance prepared using surface activation10.1016/j.actbio.2019.04.048 ↗
37. Enhanced corrosion resistance and biocompatibility of polydopamine/dicalcium phosphate dihydrate/collagen composite coating on magnesium alloy for orthopedic applications10.1016/j.jallcom.2019.152782 ↗
38. Versatile bioactive and antibacterial coating system based on silica, gentamicin, and chitosan: Improving early stage performance of titanium implants10.1016/j.surfcoat.2019.125138 ↗
39. The controversial antibacterial activity of graphene-based materials10.1016/j.carbon.2016.04.046 ↗
40. Antibacterial activity and reusability of CNT-Ag and GO-Ag nanocomposites10.1016/j.apsusc.2013.06.086 ↗
41. Structure–process–property relationship of the polar graphene oxide-mediated cellular response and stimulated growth of osteoblasts on hybrid chitosan network structure nanocomposite scaffolds10.1016/j.actbio.2011.05.019 ↗
42. A graphene oxide-Ag co-dispersing nanosystem: Dual synergistic effects on antibacterial activities and mechanical properties of polymer scaffolds10.1016/j.cej.2018.04.092 ↗
43. Dziková, J., Fintová, S., Kajánek, D., Florková, Z., Wasserbauer, J., and Doležal, P. (2021). Characterization and corrosion properties of fluoride conversion coating prepared on AZ31 magnesium alloy. Coatings, 11.10.3390/coatings11060675 ↗
44. Wang, Z., Ye, F., Chen, L., Lv, W., Zhang, Z., Zang, Q., Peng, J., Sun, L., and Lu, S. (2021). Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid. Coatings, 11.10.3390/coatings11060667 ↗
45. Anawati, A., Fitriana, M., and Gumelar, M. (2021). Improved Corrosion Resistance of Magnesium Alloy AZ31 in Ringer Lactate by Bilayer Anodic Film/Beeswax–Colophony. Coatings, 11.10.3390/coatings11050564 ↗
46. Wei, J., Cai, S., Li, Q., Xie, Y., Bao, X., and Xu, G. (2020). Controllable Synthesis of Nanostructured Ca-P Coating on Magnesium Alloys via Sodium Citrate Template-Assisted Hydrothermal Method and Its Corrosion Resistance. Coatings, 10.10.3390/coatings10121232 ↗
47. Zhang, R., Zhang, Z., Zhu, Y., Zhao, R., Zhang, S., Shi, X., Li, G., Chen, Z., and Zhao, Y. (2020). Degradation Resistance and In Vitro Cytocompatibility of Iron-Containing Coatings Developed on WE43 Magnesium Alloy by Micro-Arc Oxidation. Coatings, 10.10.3390/coatings10111138 ↗
48. Toulabifard, A., Rahmati, M., Raeissi, K., Hakimizad, A., and Santamaria, M. (2020). The Effect of Electrolytic Solution Composition on the Structure, Corrosion, and Wear Resistance of PEO Coatings on AZ31 Magnesium Alloy. Coatings, 10.10.3390/coatings10100937 ↗
49. Wang, S., Fu, L., Nai, Z., Liang, J., and Cao, B. (2018). Comparison of Corrosion Resistance and Cytocompatibility of MgO and ZrO2 Coatings on AZ31 Magnesium Alloy Formed via Plasma Electrolytic Oxidation. Coatings, 8.10.3390/coatings8120441 ↗
50. Hou, S., Yu, W., Yang, Z., Li, Y., Yang, L., and Lang, S. (2020). Properties of Titanium Oxide Coating on MgZn Alloy by Magnetron Sputtering for Stent Application. Coatings, 10.10.3390/coatings10100999 ↗
51. Li, S., Yi, L., Zhu, X., and Liu, T. (2020). Ultrasonic Treatment Induced Fluoride Conversion Coating without Pores for High Corrosion Re-sistance of Mg Alloy. Coatings, 10.10.3390/coatings10100996 ↗
52. Fu, L., Yang, Y., Zhang, L., Wu, Y., Liang, J., and Cao, B. (2019). Preparation and Characterization of Fluoride-Incorporated Plasma Electrolytic Oxidation Coatings on the AZ31 Magnesium Alloy. Coatings, 9.10.3390/coatings9120826 ↗