Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts
Source abstract
Abstract
This study is focused on the adhesion and differentiation of the human primary mesenchymal stem cells (hMSC) to osteoblasts lineage on biological-derived hydroxyapatite (BHA) and lithium-doped BHA (BHA:LiP) coatings synthesized by Pulsed Laser Deposition. An optimum adhesion of the cells on the surface of BHA:LiP coatings compared to control (uncoated Ti) was demonstrated using immunofluorescence labelling of actin and vinculin, two proteins involved in the initiation of the cell adhesion process. BHA:LiP coatings were also found to favor the differentiation of the hMSC towards an osteoblastic phenotype in the presence of osteoinductive medium, as revealed by the evaluation of osteoblast-specific markers, osteocalcin and alkaline phosphatase. Numerous nodules of mineralization secreted from osteoblast cells grown on the surface of BHA:LiP coatings and a 3D network-like organization of cells interconnected into the extracellular matrix were evidenced. These findings highlight the good biocompatibility of the BHA coatings and demonstrate that the use of lithium as a doping agent results in an enhanced osteointegration potential of the synthesized biomaterials, which might therefore represent viable candidates for future in vivo applications.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · 3D Printing in Biomedical Research · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Biochemistry, Romanian Academy, 060031 Bucharest, Romania
National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Center for Advanced Laser Technologies (CETAL), National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
Center of Nanotechnology & Biomaterials Application & Research, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey
School of Engineering and Design, Brunel University, London UB8 3PH, UK
References (49)
Show source-provided references
1. Metallic Scaffolds for Bone Regeneration10.3390/ma2030790 ↗
2. Biomaterials and interface with bone10.1007/s00198-011-1618-x ↗
3. Tuning surface properties of bone biomaterials to manipulate osteoblastic cell adhesion and the signaling pathways for the enhancement of early osseointegration10.1016/j.colsurfb.2018.01.022 ↗
4. Classification of osseointegrated implant surfaces: Materials, chemistry and topography10.1016/j.tibtech.2009.12.003 ↗
5. Current trends in dental implants10.5125/jkaoms.2014.40.2.50 ↗
6. Fluoride-containing bioactive glasses: Glass design, structure, bioactivity, cellular interactions, and recent developments10.1016/j.msec.2015.08.064 ↗
7. Biphasic calcium phosphate ceramics for bone reconstruction: A review of biological response10.1016/j.actbio.2017.01.076 ↗
8. Calcium phosphate-based coatings on titanium and its alloys10.1002/jbm.b.30932 ↗
9. Protein adsorption and osteoblast precursor cell attachment to hydroxyapatite of different crystallinities
10. Graziani, G., Boi, M., and Bianchi, M. (2018). A Review on ionic substitutions in hydroxyapatite thin films: towards complete biomimetism. Coatings, 8.10.3390/coatings8080269 ↗
11. Vlădescu, A., Pârâu, A., Pană, I., Cotruț, C.M., Constantin, L.R., Braic, V., and Vrânceanu, D.M. (2019). In vitro activity assays of sputtered HAp coatings with SiC addition in various simulated biological fluids. Coatings, 9.10.3390/coatings9060389 ↗
12. Jonauske, V., Stanionyte, S., Chen, S.-W., Zarkov, A., Juskenas, R., Selskis, A., Matijosius, T., Yang, T.C.K., Ishikawa, K., and Ramanauskas, R. (2019). Characterization of sol-gel derived calcium hydroxyapatite coatings fabricated on patterned rough stainless steel surface. Coatings, 9.10.3390/coatings9050334 ↗
13. León, B., and Jansen, J.A. (2009). Thin Calcium Phosphate Coatings for Medical Implants, Springer.10.1007/978-0-387-77718-4 ↗
14. Characterization of sol-gel derived silver/fluor-hydroxyapatite composite coatings on titanium substrate10.1016/j.surfcoat.2018.08.021 ↗
15. Scaffolds for Bone Tissue Engineering: State of the art and new perspectives10.1016/j.msec.2017.05.017 ↗
16. Mariani, E., Lisignoli, G., Borzì, R.M., and Pulsatelli, L. (2019). Biomaterials: Foreign Bodies or Tuners for the Immune Response?. Int. J. Mol. Sci., 20.10.3390/ijms20030636 ↗
17. Osteoimmunomodulation for the development of advanced bone biomaterials10.1016/j.mattod.2015.11.004 ↗
18. Tite, T., Popa, A.C., Balescu, L.M., Bogdan, I.M., Pasuk, I., Ferreira, J.M.F., and Stan, G.E. (2018). Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods. Materials, 11.10.3390/ma11112081 ↗
19. Duta, L., and Popescu, A.C. (2019). Current Status on Pulsed Laser Deposition of Coatings from Animal-Origin Calcium Phosphate Sources. Coatings, 9.10.3390/coatings9050335 ↗
20. Chrisey, D.B., and Hubler, G.K. (1994). Pulsed Laser Deposition of Thin Films, John Wiley & Sons. [1st ed.].
21. Eason, R. (2006). Pulsed Laser Deposition of Thin Films-Applications-Led Growth of Functional Materials, Wiley-Interscience.10.1002/0470052120 ↗
22. Recent advances in pulsed-laser deposition of complex oxides10.1088/0953-8984/20/26/264005 ↗
23. Physical-chemical characterization and biological assessment of simple and lithium-doped biological-derived hydroxyapatite thin films for a new generation of metallic implants10.1016/j.apsusc.2018.01.008 ↗
24. Duta, L., Chifiriuc, M.C., Popescu-Pelin, G., Bleotu, C., (Pircalabioru) Gradisteanu, G., Anastasescu, M., Achim, A., and Popescu, A. (2019). Pulsed Laser Deposited Biocompatible Lithium-Doped Hydroxyapatite Coatings with Antimicrobial Activity. Coatings, 9.10.3390/coatings9010054 ↗
25. Stem cells10.1016/S0140-6736(05)66879-1 ↗
26. Comparison of mesenchymal stem cell and osteosarcoma cell adhesion to hydroxyapatite10.1007/s10856-008-3525-z ↗
27. Comparative physical, chemical and biological assessment of simple and titanium-doped ovine dentine-derived hydroxyapatite coatings fabricated by pulsed laser deposition10.1016/j.apsusc.2017.04.025 ↗
28. Novel doped hydroxyapatite thin films obtained by pulsed laser deposition10.1016/j.apsusc.2012.10.077 ↗
29. Eisenhart, S. (2013). EU Regulation 722. New EU Animal Tissue Regulations in Effect for Some Medical Devices, Emergo. Available online: https://www.emergobyul.com/blog/2013/09/new-eu-animaltissue-regulations-effect-some-medical-devices.
30. ISO 22442-1 (2015). Medical Devices Utilizing Animal Tissues and Their Derivatives—Part 1: Application of Risk Management, International Organization for Standardization.
31. Biocompatibility evaluation of a novel hydroxyapatite-polymer coating for medical implants (in vitro tests)
32. Specific biofunctional performances of the hydroxyapatite–sodium maleate copolymer hybrid coating nanostructures evaluated by in vitro studies10.1007/s10856-009-3800-7 ↗
33. An Alizarin red-based assay of mineralization by adherent cells in culture: Comparison with cetylpyridinium chloride extraction10.1016/j.ab.2004.02.002 ↗
34. Differentiation of mesenchymal stem cells onto highly adherent radio frequency-sputtered carbonated hydroxylapatite thin films10.1002/jbm.a.32947 ↗
35. The integrin-actin connection, an eternal love affair10.1093/emboj/cdg245 ↗
36. Osteoblast adhesion on biomaterials10.1016/S0142-9612(99)00242-2 ↗
37. Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)10.1016/S0142-9612(00)00415-4 ↗
38. Mammalian alkaline phosphatases are allosteric enzymes10.1074/jbc.272.36.22781 ↗
39. Alkaline phosphatase: An overview10.1007/s12291-013-0408-y ↗
40. Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase10.1073/pnas.83.19.7182 ↗
41. Differential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cells10.1359/jbmr.1999.14.1.47 ↗
42. Chemical and topographical influence of hydroxyapatite and beta-tricalcium phosphate surfaces on human osteoblastic cell behavior
43. Comparative response of epithelial cells and osteoblasts to microfabricated tapered pit topographies in vitro and in vivo10.1016/j.biomaterials.2007.01.026 ↗
44. Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients10.1016/j.biomaterials.2007.01.019 ↗
45. Vitamin D and gene networks in human osteoblasts
46. The osteocyte: An endocrine cell... and more10.1210/er.2012-1026 ↗
47. Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests10.1016/j.apsusc.2008.10.031 ↗
48. Ex vivo construction of human primary 3D-networked osteocytes10.1016/j.bone.2017.09.012 ↗
49. Mineralization of three-dimensional osteoblast cultures is enhanced by the interaction of 1α,25-dihydroxyvitamin D3 and BMP2 via two specific vitamin D receptors10.1002/term.1770 ↗