Films of Chitosan and Aloe vera for Maintaining the Viability and Antifungal Activity of Lactobacillus paracasei TEP6
Source abstract
Abstract
The present study aimed to evaluate the effect of Aloe vera addition on the viability and antifungal activity of TEP6 (Lactobacillus paracasei) bacteria immobilized on chitosan films for 28 days. Different chitosan and A. vera proportions and carbon sources at several pH values were tested as formulations for supporting the microorganism. Bacterial viability was maintained in freshly made films, with values of 10.4, 10.8 and 10.9 log CFU·g−1 for the formulations containing 70% (T11), 100% (T8) and 100% (T16) of A. vera, respectively. The same formulations (T8, T11 and T16) maintained bacterial viability for 14 days of film storage with a loss to values of 9.5 log CFU·g−1. By applying a quarter fraction 25–2 experimental design with an array of five factors, the factors with the greatest influence on viability and antifungal activity were determined. The optimal conditions for viability were the formulation with 100% A. vera, pH 4.5 and 0.1 M glucose. The antifungal activity of fresh films was influenced by the formulation with 10 g·L−1 glycerol and 100% A. vera, showing a 60% inhibition of fungal (Colletotrichum gloeosporioides) growth. The films developed in this study may have the potential to be used as coatings on vegetal products susceptible to attack by Colletotrichum gloesporioides.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Probiotics and Fermented Foods · Nanocomposite Films for Food Packaging · Essential Oils and Antimicrobial Activity
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Instituto de Biociencias, Universidad Autónoma de Chiapas, Boulevard Príncipe Akishino sin número Colonia Solidaridad 2000, Tapachula CP 30798, Mexico
References (29)
Show source-provided references
1. Antibacterial and antibiofilm activity of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China against enteropathogenic bacteria10.1007/s12602-017-9364-9 ↗
2. Exploiting antagonistic activity of fruit-derived Lactobacillus to control pathogenic bacteria in fresh cheese and chicken meat10.1016/j.foodres.2018.03.045 ↗
3. Antifungal activity of lactic acid bacteria and their application for Fusarium mycotoxin reduction in malting wheat grains10.1016/j.lwt.2017.10.061 ↗
4. In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum10.1016/j.lwt.2017.03.053 ↗
5. Lactic acid bacteria incorporated into edible coatings to control fungal growth and maintain postharvest quality of grapes10.21273/HORTSCI13661-18 ↗
6. Biological control of fire blight of apple and pear with antagonistic Lactobacillus plantarum10.1007/s10658-013-0275-7 ↗
7. Antifungal lactic acid bacteria isolated from fermented beverages with activity against Colletotrichum gloeosporioides10.1016/j.fbio.2019.03.008 ↗
8. Microencapsulation of probiotic bacteria and its potential application in food technology10.5958/j.2230-732X.7.1.007 ↗
9. Probiotics and their potential applications in active edible films and coatings10.1016/j.foodres.2016.10.026 ↗
10. Edible films as carrier for lactic acid bacteria10.1016/j.lwt.2016.06.060 ↗
11. Effect of chitosan—Aloe vera coating on postharvest quality of blueberry (Vaccinium corymbosum) fruit10.1016/j.postharvbio.2016.01.011 ↗
12. Effects of inulin, fructooligosacharides/glucose and pH on the shape of the death kinetic of Lactobacillus reuteri DSM 2001610.1111/ijfs.13205 ↗
13. Composite edible films and coatings from food-grade biopolymers10.1007/s13197-018-3402-9 ↗
14. Development of chitosan based optimized edible coating for tomato (Solanum lycopersicum) and its characterization10.1007/s13197-018-3162-6 ↗
15. Chitosan as a preservative for fruits and vegetables: A review on chemistry and antimicrobial properties10.21967/jbb.v4i1.189 ↗
16. Suitability of Aloe vera and gum tragacanth as edible coatings for extending the shelf life of button mushroom10.1007/s11947-011-0709-1 ↗
17. Effect of Aloe vera juice on growth and activities of Lactobacilli in-vitro
18. Effect of different Aloe fractions on the growth of lactic acid bacteria10.1111/1750-3841.13568 ↗
19. Characterization of Aloe vera-chitosan composite films and their use for reducing the disease caused by fungi in papaya Maradol10.1007/s13197-018-3397-2 ↗
20. Improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay., for measuring the viability of lactic acid bacteria10.1556/AAlim.41.2012.4.12 ↗
21. Fermentation factors influencing the production of bacteriocins by lactic acid bacteria: A review10.1039/C6RA24579J ↗
22. Characterization of chitosan nanofiber sheets for antifungal application10.3390/ijms161125947 ↗
23. Combination of uv-c radiation and chitosan films enriched with essential oils for fungi control in papaya ‘Maradol’
24. Synbiotic effect of various prebiotics on in vitro activities of probiotic lactobacilli10.1080/03670244.2011.539161 ↗
25. The effect of controlled and uncontrolled pH cultures on the growth of Lactobacillus delbrueckii subsp10.1016/j.lwt.2016.11.057 ↗
26. Influence of culture media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria10.1186/s13568-018-0536-0 ↗
27. The effects of different carbon sources on the antifungal activity by lactic acid bacteria10.1007/s00284-016-1168-8 ↗
28. Physical and nutritional conditions for optimized production of bacteriocins by lactic acid bacteria—A review10.1080/10408398.2018.1474852 ↗
29. Bravo-De la Cruz, C.M., Rosas-Quijano, R., Gálvez-López, D., Albores-Flores, V., and Vázquez-Ovando, A. Antifungal ability of chitosan biofilms containing lactic acid bacteria. J. Negat. No Posit. Results, in press.