The Bioactive Polypyrrole/Polydopamine Nanowire Coating with Enhanced Osteogenic Differentiation Ability with Electrical Stimulation
Source abstract
Abstract
Polypyrrole (PPy) is a promising conducting polymer in bone regeneration; however, due to the biological inertia of the PPy surface, it has poor cell affinity and bioactivity. Based on the excellent adhesion capacity, biocompatibility, and bioactivity of polydopamine (PDA), the PDA is used as a functional coating in tissue repair and regeneration. Herein, we used a two-step method to construct a functional conductive coating of polypyrrole/polydopamine (PPy/PDA) nanocomposite for bone regeneration. PPy nanowires (NWs) are used as the morphologic support layer, and a layer of highly bioactive PDA is introduced on the surface of PPy NWs by solution oxidation. By controlling the depositing time of PDA within 5 h, the damage of nano morphology and conductivity of the PPy NWs caused by the coverage of PDA deposition layer can be effectively avoided, and the thin PDA layer also significantly improve the hydrophilicity, adhesion, and biological activity of PPy NWs coating. The PPy/PDA NWs coating performs better biocombaitibility and bioactivity than pure PPy NWs and PDA, and has benefits for the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells cultured on the surface. In addition, PPy/PDA NWs can significantly promote the osteogenesis of MC3T3-E1 in combination with micro galvanostatic electrical stimulation (ES).
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Polymer Surface Interaction Studies · Conducting polymers and applications · Electrospun Nanofibers in Biomedical Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China
Quanzhou Institute of Equipment Manufacturing Haixi Institutes, Chinese Academy of Science, Quanzhou 362000, China
Funding
National Nature Foundation of China · National Nature Science Foundation of China · Research Funds for the Central Universities
References (39)
Show source-provided references
1. Engineering antifouling conducting polymers for modern biomedical applications10.1021/acsami.9b04924 ↗
2. Functionalization of conducting polymers for biointerface applications10.1016/j.progpolymsci.2017.03.004 ↗
3. Biomimetic conducting polymer-based tissue scaffolds10.1016/j.copbio.2013.03.011 ↗
4. Conducting polymer nanostructures and their application in biosensors10.1016/j.jcis.2009.09.029 ↗
5. Tuning drug delivery from conducting polymer films for accurately controlled release of charged molecules10.1016/j.jconrel.2019.05.017 ↗
6. Enhanced drug loading capacity of polypyrrole nanowire network for controlled drug release10.1016/j.synthmet.2012.12.010 ↗
7. The effect of polypyrrole on arteriogenesis in an acute rat infarct model10.1016/j.biomaterials.2008.07.021 ↗
8. A conductive sodium alginate and carboxymethyl chitosan hydrogel doped with polypyrrole for peripheral nerve regeneration10.1039/C8RA01059E ↗
9. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications10.1016/j.biomaterials.2009.04.042 ↗
10. Harjo, M., Torop, J., Järvekülg, M., Tamm, T., and Kiefer, R. (2019). Electrochemomechanical behavior of polypyrrole-coated nanofiber scaffolds in cell culture medium. Polymers, 11.10.3390/polym11061043 ↗
11. Electrical stimulation to promote osteogenesis using conductive polypyrrole films10.1016/j.msec.2013.12.019 ↗
12. Polypyrrole nanocones and dynamic piezoelectric stimulation-induced stem cell osteogenic differentiation10.1021/acsbiomaterials.9b00812 ↗
13. Electrical stimulation of human mesenchymal stem cells on biomineralized conducting polymers enhances their differentiation towards osteogenic outcomes10.1039/C5TB00714C ↗
14. A mussel-inspired persistent ROS-scavenging, electroactive, and osteoinductive scaffold based on electrochemical-driven in situ nanoassembly10.1002/smll.201805440 ↗
15. Improved in vitro angiogenic behavior of human umbilical vein endothelial cells with oxidized polydopamine coating10.1016/j.colsurfb.2020.111176 ↗
16. The adhesive proteins secreted by mussels are the inspiration behind a versatile approach to the surface modification of a wide range of inorganic and organic materials, resulting in the fabrication of multifunctional coatings for a variety of applications10.1038/nmat2087 ↗
17. A facile in situ approach to polypyrrole functionalization through bioinspired catechols10.1002/adfm.201403115 ↗
18. Polydopamine-modified black phosphorous nanocapsule with enhanced stability and photothermal performance for tumor multimodal treatments10.1002/advs.201800510 ↗
19. Cost-effective strategy for surface modification via complexation of disassembled polydopamine with Fe(III) ions10.1021/acs.langmuir.9b00245 ↗
20. Role of surfactants in the control of dopamine–eumelanin particle size and in the inhibition of film deposition at solid–liquid interfaces10.1016/j.jcis.2014.06.025 ↗
21. Bio-inspired dopamine functionalization of polypyrrole for improved adhesion and conductivity10.1002/marc.201300761 ↗
22. Electrochemical deposition of conductive and adhesive polypyrrole-dopamine films10.1038/srep30475 ↗
23. Stretchable and highly sensitive braided composite yarn@polydopamine@polypyrrole for wearable applications10.1021/acsami.8b18823 ↗
24. Electrically conductive polydopamine-polypyrrole as high performance biomaterials for cell stimulation in vitro and electrical signal recording in vivo10.1021/acsami.8b11546 ↗
25. Morphologically controlled bioinspired dopamine-polypyrrole nanostructures with tunable electrical properties10.1002/aelm.201500205 ↗
26. Electroresponsive and cell-affinitive polydopamine/polypyrrole composite microcapsules with a dual-function of on-demand drug delivery and cell stimulation for electrical therapy10.1038/am.2017.16 ↗
27. Polydopamine-assisted electrochemical fabrication of polypyrrole nanofibers on bone implants to improve bioactivity10.1002/mame.201600285 ↗
28. Electrochemical synthesis of conductive. superhydrophobic and adhesive polypyrrole-polydopamine nanowires10.1016/j.synthmet.2017.10.012 ↗
29. Conducting polymernanowire arrays with enhanced electrochemical performance10.1039/B919928D ↗
30. Template-free one-step electrochemical formation of polypyrrole nanowire array10.1016/j.elecom.2008.11.030 ↗
31. Polydopamine-assisted deposition of polypyrrole on electrospun poly(vinylidene fluoride) nanofibers for bidirectional removal of cation and anion dyes10.1016/j.cej.2018.08.048 ↗
32. Mussel-inspired surface chemistry for multifunctional coatings10.1126/science.1147241 ↗
33. Anti-corrosion and conductivity of the electrodeposited graphene/polypyrrole composite coating for metallic bipolar plates10.1016/j.porgcoat.2019.105237 ↗
34. Improved immobilization of biomolecules to quinone-rich polydopamine for efficient surface functionalization10.1016/j.colsurfb.2013.01.033 ↗
35. Characterization of polydopamine thin films deposited at short times by autoxidation of dopamine10.1021/la400587j ↗
36. Distinct proliferative and differentiated stages of murine MC3T3-El cells in culture: An in vitro model of osteoblast development10.1002/jbmr.5650070613 ↗
37. Low-intensity electrical stimulation counteracts the effects of ovariectomy on bone tissue of rats: Effects on bone microarchitecture, viability of osteocytes, and nitric oxide expression10.1007/s00223-009-9227-9 ↗
38. Is nitric oxide a mediator of the effects of low-intensity electrical stimulation on bone in ovariectomized rats?10.1007/s00223-010-9357-0 ↗
39. Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation10.1016/j.msec.2016.09.067 ↗