Essential Oils and Essential Oil-Based Products Compared to Chemical Biocides Against Microbial Patinas on Stone Cultural Heritage
Source abstract
Abstract
In recent years, attention has been paid to finding eco-friendly products that could represent an alternative for the chemicals usually used during conservation procedures. In the field of the devitalization of biological patinas, the most studied products are essential oils (EOs). Several works tested EOs in vitro on microorganisms isolated from stone artifacts but few applied these products on real artworks colonized by a complex patina. In the present work, the biocidal effect of two EOs (cinnamon bark and oregano) and two EO-based products (Biotersus and Essenzio) was compared to that of three chemical biocides commonly used in the conservation of stone artifacts (Biotin R1+R2, NewDes50, and Preventol RI50). The products were applied by brush in situ on a marble slab characterized by a green patina. The biocidal activity was evaluated by analyzing the chlorophyll fluorescence and quantifying ATP through biochemical tests. Furthermore, the products’ interference with stone was evaluated by colorimetric measures on sedimentary rock samples. The results indicate that chemical biocides exhibited biocidal activity of greater than 99.5% with a single application, while cinnamon oil (at a 1% concentration) and Essenzio required two applications to achieve the same level of efficacy. Conversely, oregano oil and Biotersus were not able to effectively reduce microorganism vitality, even with repeated applications.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Building materials and conservation · Conservation Techniques and Studies · Cultural Heritage Materials Analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Biology Laboratory, Istituto Centrale per il Restauro (ICR), Ministry of Culture (MIC), Via di San Michele 25, 00153 Rome, Italy
Bio.Co.Ré. Lab, Via Reatina 10, 67068 Scurcola Marsicana, Italy
Appia Antica Archaeological Park, Piazza delle Finanze 1, 00185 Rome, Italy
Stone Materials Laboratory, Istituto Centrale per il Restauro (ICR), Ministry of Culture (MIC), Via di San Michele 25, 00153 Rome, Italy
Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy
Funding
European Union-NextGenerationEU
References (43)
Show source-provided references
1. Pinna, D. (2017). Coping with Biological Growth on Stone Heritage Objects: Methods, Products, Applications, and Perspectives, Taylor and Francis.10.1201/9781315365510 ↗
2. Microbial Deterioration of Stone Monuments—An Updated Overview10.1016/S0065-2164(08)00805-8 ↗
3. The Lithobiontic Ecological Niche, with Special Reference to Microorganisms
4. Tiano, P. (2002, January 5–6). Biodegradation of Cultural Heritage: Decay Mechanisms and Control Methods. Proceedings of the 9th ARIADNE Workshop, Bath, UK.
5. Characterization of Epilithic Microbial Communities Associated with Mayan Stone Monuments in Yucatán, Mexico10.1080/014904599270604 ↗
6. Natural Biocides for the Conservation of Stone Cultural Heritage: A Review10.1016/j.culher.2019.01.005 ↗
7. Antonelli, F., Bartolini, M., Plissonnier, M.-L., Esposito, A., Galotta, G., Ricci, S., Davidde Petriaggi, B., Pedone, C., Di Giovanni, A., and Piazza, S. (2020). Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological Wood. Microorganisms, 8.10.3390/microorganisms8122015 ↗
8. Marine Organisms as Source of Bioactive Molecules Applied in Restoration Projects10.1186/s40494-015-0046-1 ↗
9. A First Assessment of the Biocidal Efficacy of Plant Essential Oils against Lichens on Stone Cultural Heritage, and the Importance of Evaluating Suitable Application Protocols10.1016/j.culher.2022.02.006 ↗
10. Minotti, D., Vergari, L., Proto, M.R., Barbanti, L., Garzoli, S., Bugli, F., Sanguinetti, M., Sabatini, L., Peduzzi, A., and Rosato, R. (2022). Il Silenzio: The First Renaissance Oil Painting on Canvas from the Uffizi Museum Restored with a Safe, Green Antimicrobial Emulsion Based on Citrus aurantium var. amara Hydrolate and Cinnamomum zeylanicum Essential Oil. J. Fungi, 8.10.3390/jof8020140 ↗
11. Argyri, A.A., Doulgeraki, A.I., Varla, E.G., Bikouli, V.C., Natskoulis, P.I., Haroutounian, S.A., Moulas, G.A., Tassou, C.C., and Chorianopoulos, N.G. (2021). Evaluation of Plant Origin Essential Oils as Herbal Biocides for the Protection of Caves Belonging to Natural and Cultural Heritage Sites. Microorganisms, 9.10.3390/microorganisms9091836 ↗
12. Cirone, M., Figoli, A., Galiano, F., La Russa, M.F., Macchia, A., Mancuso, R., Ricca, M., Rovella, N., Taverniti, M., and Ruffolo, S.A. (2023). Innovative Methodologies for the Conservation of Cultural Heritage against Biodeterioration: A Review. Coatings, 13.10.3390/coatings13121986 ↗
13. Reale, R., Medeghini, L., and Botticelli, M. (2024). Stealing from Phytotherapy—Heritage Conservation with Essential Oils: A Review, from Remedy to Sustainable Restoration Product. Sustainability, 16.10.3390/su16125110 ↗
14. Biodeterioration of Stone Monuments: Studies on the Influence of Bioreceptivity on Cyanobacterial Biofilm Growth and on the Biocidal Efficacy of Essential Oils in Natural Hydrogel10.1016/j.scitotenv.2023.161901 ↗
15. Ranaldi, R., Rugnini, L., Gabriele, F., Spreti, N., Casieri, C., Di Marco, G., Gismondi, A., and Bruno, L. (2022). Plant Essential Oils Suspended into Hydrogel: Development of an Easy-to-Use Protocol for the Restoration of Stone Cultural Heritage. Int. Biodeterior. Biodegrad., 172.10.1016/j.ibiod.2022.105436 ↗
16. Bartoli, F., Corradi, L., Hosseini, Z., Privitera, A., Zuena, M., Kumbaric, A., Graziani, V., Tortora, L., Sodo, A., and Caneva, G. (2024). In Vitro Viability Tests of New Ecofriendly Nanosystems Incorporating Essential Oils for Long-Lasting Conservation of Stone Artworks. Gels, 10.10.3390/gels10020132 ↗
17. Biological Effects of Essential Oils—A Review10.1016/j.fct.2007.09.106 ↗
18. Genotoxicity and Antigenotoxicity of Origanum Oil and Carvacrol Evaluated by Ames Salmonella/Microsomal Test10.1016/j.foodchem.2004.12.034 ↗
19. Insecticidal and Genotoxic Activities of Mint Essential Oils10.1021/jf960685f ↗
20. Potent Antimutagenic Activity of White Tea in Comparison with Green Tea in the Salmonella Assay10.1016/S1383-5718(01)00200-5 ↗
21. Antitrypanosomal Compounds from the Leaf Essential Oil of Strychnos spinosa10.1055/s-2005-916255 ↗
22. Effect of Essential Oils on Pathogenic Bacteria10.3390/ph6121451 ↗
23. Essential Oils of Aromatic Plants with Antibacterial, Antifungal, Antiviral, and Cytotoxic Properties—An Overview
24. Major Bioactivities and Mechanism of Action of Essential Oils and Their Components10.1002/ffj.3165 ↗
25. Can Baser, K.H., and Buchbauer, G. (2009). Handbook of Essential Oils, CRC Press.10.1201/9781420063165 ↗
26. Spada, M., Cuzman, O.A., Tosini, I., Galeotti, M., and Sorella, F. (2021). Essential Oils Mixtures as an Eco-Friendly Biocidal Solution for a Marble Statue Restoration. Int. Biodeterior. Biodegrad., 163.10.1016/j.ibiod.2021.105280 ↗
27. Devreux, G., Santamaria, U., Morresi, F., Rodolfo, A., Barbabietola, N., Fratini, F., and Reale, R. (2015, January 22–24). Fitoconservazione. Trattamenti Alternativi Sulle Opere in Materiale Lapideo Nei Giardini Vaticani. Proceedings of the XIII Congresso Nazionale IGIIC—Lo Stato dell’Arte, Torino, Italy.
28. La Disinfezione Delle Patine Biologiche Sui Manufatti Lapidei: Biocidi Chimici e Naturali a Confronto
29. Macchia, A., Aureli, H., Prestileo, F., Ortenzi, F., Sellathurai, S., Docci, A., Cerafogli, E., Colasanti, I.A., Ricca, M., and La Russa, M.F. (2022). In-Situ Comparative Study of Eucalyptus, Basil, Cloves, Thyme, Pine Tree, and Tea Tree Essential Oil Biocide Efficacy. Methods Protoc., 5.10.3390/mps5030037 ↗
30. Spada, M., Sorella, F., Galeotti, M., Tosini, I., and Cuzman, O.A. (2021). Non-Invasive Technologies to Timely Screen out Different Application Conditions of Essential Oils on Stone. Int. Biodeterior. Biodegrad., 163.10.1016/j.ibiod.2021.105285 ↗
31. Romano, I., Granata, G., Poli, A., Finore, I., Napoli, E., and Geraci, C. (2020). Inhibition of Bacterial Growth on Marble Stone of 18th Century by Treatment of Nanoencapsulated Essential Oils. Int. Biodeterior. Biodegrad., 148.10.1016/j.ibiod.2020.104909 ↗
32. Gagliano Candela, R., Maggi, F., Lazzara, G., Rosselli, S., and Bruno, M. (2019). The Essential Oil of Thymbra capitata and Its Application as A Biocide on Stone and Derived Surfaces. Plants, 8.10.3390/plants8090300 ↗
33. Biodeterioration of Roman Hypogea: The Case Study of the Catacombs of SS. Marcellino and Pietro (Rome, Italy)10.1007/s13213-019-01460-z ↗
34. Essential Oils to Contrast Biodeterioration of the External Marble of Florence Cathedral10.1016/j.scitotenv.2023.162913 ↗
35. CTS srl (2021). BIOTIN R1 + R2 Nuovo Sistema Preservante a Due Componenti Concentrat i per Materiali Organici e Da Costruzione, Diluibile in Solvente, CTS srl.
36. CTS srl (2013). New Des 50 Preservante Concentrato a Base Di Sali Quaternari d’Ammonio per Materiali Organici e Da Costruzione, CTS srl.
37. Lanxess (2011). Preventol RI80/RI50, Lanxess.
38. Exentiae (2024, November 04). Biotersus. Available online: https://www.exentiae.it/en/biotersus/.
39. Ibix Biocare (2024, November 04). Essenzio. Available online: https://www.ibixbiocare.it/it/prodotti/essenzio.
40. Color Difference ΔE—A Survey
41. Georgali, B., and Toumbakari, E.E. (2001, January 4–7). Application of Fluorescence Microscopy to Evaluate the Vitality of Photoautotrophic Biodeteriogens. Proceedings of the 8th Euroseminar on Microscopy Applied to Building Materials, Athens, Greece.
42. Detection of Viable Fungal Spores Contaminant on Documents and Rapid Control of the Effectiveness of an Ethylene Oxide Disinfection Using ATP Assay10.1002/bio.710 ↗
43. Driussi, G. (2023). Studio Di Prodotti Naturali per La Devitalizzazione Dei Licheni Incrostanti Sulle Pavimentazioni in Opus Sectile in Un Contesto Archeologico. Intervention on Historic Building Surfaces. What About Innovation?, Edizione Arcadia Ricerche Srl.