Multi-Level Evaluation of UV Action upon Vitamin D Enhanced, Silver Doped Hydroxyapatite Thin Films Deposited on Titanium Substrate
Source abstract
Abstract
Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) is an important bioactive material for bone tissue reconstruction, due to its highly thermodynamic stability at a physiological pH without bio-resorption. In the present study, the Ag:HAp and the corresponding Ag:HAp + D3 thin films (~200 nm) coating were obtained by vacuum deposition method on Ti substrate. The obtained samples were exposed to different UV irradiation times, in order to investigate the UV light action upon thin films, before considering this method for the thin film’s decontamination. The effects of UV irradiation upon Ag:Hap + D3 are presented for the first time in the literature, marking a turning point for understanding the effect of UV light on composite biomaterial thin films. The UV irradiation induced an increase in the initial stages of surface roughness of Ag:HAp thin film, correlated with the modifications of XPS and FTIR signals. The characteristics of thin films measured by AFM (RMS) analysis corroborated with XPS and FTIR investigation highlighted a process of recovery of the thin film’s properties (e.g., RMS), suggesting a possible adaptation to UV irradiation. This process has been a stage to a more complicated UVA rapid degradation process. The antifungal assays demonstrated that all the investigated samples exhibited antifungal properties. Moreover, the cytotoxicity assays revealed that the HeLa cells morphology did not show any alterations after 24 h of incubation with the Ag:HAp and Ag:HAp + D3 thin films.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental materials and restorations · Additive Manufacturing and 3D Printing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Multifunctional Materials and Structures Laboratory, National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania
Department of Surgery, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari, Sector 5, 050474 Bucharest, Romania
Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine, 59 Marasti Blvd., 011464 Bucharest, Romania
Faculty of Exact Sciences & Engineering, Hyperion University of Bucharest, 169 Calea Călărași, 030615 Bucharest, Romania
National Centre for Micro and Nanomaterials, University Politehnica of Bucharest, 060042 Bucharest, Romania
Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, Sector 5, 050097 Bucharest, Romania
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
“Stefan S Nicolau” Institute of Virology, Romanian Academy, 030304 Bucharest, Romania
Life, Environmental and Earth Sciences Division, Research Institute of the University of Bucharest (ICUB), University of Bucharest, 060023 Bucharest, Romania
Microbiology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, 77206 Bucharest, Romania
Plasma and Radiation Physics, National Institute for Laser, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania
Academy of Romanian Scientists, Ilfov Street, No. 3, 010164 Bucharest, Romania
Funding
Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii
References (78)
Show source-provided references
1. Bioceramics of calcium orthophosphates10.1016/j.biomaterials.2009.11.050 ↗
2. Electrical properties of hydroxyapatite thin films grown by pulsed laser deposition10.1016/S0040-6090(96)09146-8 ↗
3. Ultrasound studies on magnetic fluids based on maghemite nanoparticles10.1002/pen.24501 ↗
4. Predoi, D., Iconaru, S.L., Predoi, M.V., and Motelica-Heino, M. (2020). Removal and oxidation of As(III) from water using iron oxide coated ctab as adsorbent. Polymers, 12.10.3390/polym12081687 ↗
5. A review of plasma-assisted methods for calcium phosphate-based coating fabrication10.1016/j.surfcoat.2011.11.002 ↗
6. Predoi, D., Iconaru, S.L., and Predoi, M.V. (2019). Dextran-coated zinc-doped hydroxyapatite for biomedical applications. Polymers, 11.10.3390/polym11050886 ↗
7. Ciobanu, S.C., Iconaru, S.L., Predoi, D., Prodan, A.M., and Predoi, M.V. (2020). Physico-chemical properties and in vitro antifungal evaluation of samarium doped hydroxyapatite coatings. Coatings, 10.10.3390/coatings10090827 ↗
8. Iconaru, S.L., Motelica-Heino, M., Guegan, R., Predoi, M.V., Prodan, A.M., and Predoi, D. (2018). Removal of zinc ions using hydroxyapatite and study of ultrasound behavior of aqueous media. Materials, 11.10.3390/ma11081350 ↗
9. Nanosize hydroxyapatite: Doping with various ions10.1179/1743676110Y.0000000005 ↗
10. Predoi, D., Predoi, M.V., Iconaru, S.L., Ech Cherif El Kettani, M., Leduc, D., and Prodan, A.M. (2017). Ultrasonic measurements on β cyclodextrin/hydroxyapatite composites for potential water depollution. Materials, 10.10.3390/ma10060681 ↗
11. Predoi, D., Iconaru, S.L., Predoi, M.V., Groza, A., Gaiaschi, S., Rokosz, K., Raaen, S., Negrila, C.C., Prodan, A.-M., and Costescu, A. (2020). Development of cerium-doped hydroxyapatite coatings with antimicrobial properties for biomedical applications. Coatings, 10.10.3390/coatings10060516 ↗
12. Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions10.1021/cm100023p ↗
13. Silver as antibacterial agent: Ion, nanoparticle and metal10.1002/anie.201205923 ↗
14. Prodan, A.M., Iconaru, S.L., Predoi, M.V., Predoi, D., Motelica-Heino, M., Turculet, C.S., and Beuran, M. (2020). Silver-doped hydroxyapatite thin layers obtained by sol-gel spin coating procedure. Coatings, 10.10.3390/coatings10010014 ↗
15. Bone tissue engineering electrospun scaffolds based on layered double hydroxides with the ability to release vitamin D3: Fabrication, characterization and in vitro study10.1016/j.clay.2019.105434 ↗
16. Osteoblast response to Vitamin D3 loaded cellulose enriched hydroxyapatite Mesoporous silica nanoparticles composite10.1016/j.biopha.2018.04.078 ↗
17. Current perspectives on vitamin D, immune system, and chronic diseases10.1016/j.nut.2010.07.022 ↗
18. Ambrożewicz, E., Muszyńska, M., Tokajuk, G., Grynkiewicz, G., Žarković, N., and Skrzydlewska, E. (2019). Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials. Cells, 8.10.3390/cells8040325 ↗
19. Physicochemical bisphosphonate immobilization on titanium dioxide thin films surface by UV radiation for bio-application10.1016/j.surfcoat.2018.09.038 ↗
20. Chemical analysis of silica doped hydroxyapatite biomaterials consolidated by a spark plasma sintering method10.1016/j.jinorgbio.2006.09.030 ↗
21. Influence of target surface degradation on the properties of r.f. magnetron-sputtered calcium phosphate coatings10.1002/sia.1512 ↗
22. XPS study of apatite-based coatings prepared by sol-gel technique10.1016/S0169-4332(99)00267-6 ↗
23. Fabrication of uniform Ag/TiO2 nanotube array structures with enhanced photoelectrochemical performance10.1039/b9nj00780f ↗
24. X-ray photoelectron spectroscopic studies of cadmium- and silver-oxygen surfaces10.1021/ac60363a019 ↗
25. Lasting antibacterial activities of Ag–TiO2/Ag/a–TiO2 nanocomposite thin film photocatalysts under solar light irradiation10.1016/j.jcis.2009.03.018 ↗
26. Oxygen functional groups and electrochemical capacitive behavior of incompletely reduced graphene oxides as a thin-film electrode of supercapacitor10.1016/j.electacta.2013.11.040 ↗
27. XPS analaysis of food products: Toward chemical functions and molecular compounds10.1002/sia.2779 ↗
28. XPS analysis of bio-organic systems10.1002/sia.3831 ↗
29. Graphene-Nanodiamond Heterostructures and their application to High Current Devices10.1038/srep13771 ↗
30. Surface changes and radical formation on hydroxyapatite by UV irradiation for inducing photocatalytic activation10.1016/S1381-1169(03)00414-X ↗
31. Surface modification of starch based biomaterials by oxygen plasma or UV-irradiation10.1007/s10856-009-3831-0 ↗
32. Brezavšček, M., Fawzy, A., Bächle, M., Tuna, T., Fischer, J., and Att, W. (2016). The effect of uv treatment on the osteoconductive capacity of zirconia-based materials. Materials, 9.10.3390/ma9120958 ↗
33. Effective UV/Ozone irradiation method for decontamination of hydroxyapatite surfaces10.1016/j.heliyon.2017.e00372 ↗
34. Influence of ultraviolet photofunctionalization on the surface characteristics of zirconia-based dental implant materials10.1016/j.dental.2014.10.008 ↗
35. Selective laser melting of CP-Ti to overcome the low cost and high performance trade-off
36. Oxidation behavior of low-cost CP-Ti powders for additive manufacturing via fluidization10.1016/j.corsci.2020.109080 ↗
37. Analysis of Rapid Degradation in High-Power (AlGa)As Laser Diodes10.1109/3.44918 ↗
38. Structural and physical properties of antibacterial Ag-doped nano-hydroxyapatite synthesized at 100 °C10.1186/1556-276X-6-613 ↗
39. Predoi, D., Iconaru, S.L., Predoi, M.V., Motelica-Heino, M., Guegan, R., and Buton, N. (2019). Evaluation of antibacterial activity of zinc-doped hydroxyapatite colloids and dispersion stability using ultrasounds. Nanomaterials, 9.10.3390/nano9040515 ↗
40. Groza, A., Ciobanu, C.S., Popa, C.L., Iconaru, S.L., Chapon, P., Luculescu, C., Ganciu, M., and Predoi, D. (2016). structural properties and antifungal activity against Candida albicans biofilm of different composite layers based on ag/zn doped hydroxyapatite-polydimethylsiloxanes. Polymers, 8.10.3390/polym8040131 ↗
41. Predoi, D., Popa, C.L., Chapon, P., Groza, A., and Iconaru, S.L. (2016). Evaluation of the antimicrobial activity of different antibiotics enhanced with silver-doped hydroxyapatite thin films. Materials, 9.10.3390/ma9090778 ↗
42. One step synthesis of silver nanorods by autoreduction of aqueous silver ions with hydroxyapatite: An inorganic–inorganic hybrid nanocomposite10.1002/jbm.a.30895 ↗
43. Measurement the amount of Vitamin D2 (Ergocalciferol), Vitamin D3 (Cholecalciferol) and absorbable Calcium (Ca2+), Iron (II) (Fe2+), Magnesium (Mg2+), Phosphate (PO4–) and Zinc (Zn2+) in apricot using high-performance liquid chromatography (HPLC) and spectroscopic techniques10.4172/2155-6180.1000292 ↗
44. Formulation development & evaluation of effervescent tablet of alendronate sodium with vitamin D3
45. Invasive fungal infections in patients with cancer in the intensive care unit10.1016/j.ijantimicag.2011.11.017 ↗
46. Antifungal susceptibility of Candida albicans in biofilms10.1111/j.1439-0507.2011.02076.x ↗
47. Antibacterial properties of Ag-doped hydroxyapatite layers prepared by PLD method10.1007/s00339-010-5911-x ↗
48. Vibrational investigations of silver-doped hydroxyapatite with antibacterial properties10.1155/2013/471061 ↗
49. Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering10.1016/j.apsusc.2010.12.113 ↗
50. Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease10.1093/ajcn/80.6.1678S ↗
51. Evolutionary biology and pathology of vitamin D10.3177/jnsv.38.Special_79 ↗
52. (2018, January 10). Image J Software, Available online: http://imagej.nih.gov/ij.
53. Growth–inhibitory effects of 1,25-dihydroxy vitamin D3 on normal human keratinocytes cultured in serum-free medium10.1016/0006-291X(90)90898-W ↗
54. Effects of l,25-dihydroxyvitamin D3 and calcium on growth and differentiation and on c-fos and p53 gene express ion in normal human keratinocytes10.1111/1523-1747.ep12394802 ↗
55. Treatment of HL60 cells with various combinations of retinoids and 1 alpha,25 dihydroxyvitamin D3 results in differentiation towards neutrophils or monocytes or a failure to differentiate and apoptosis
56. 1,25-dihydroxyvitamin D3, transforming growth factor beta1, calcium, and ultraviolet B radiation induce apoptosis in cultured human keratinocytes10.1111/1523-1747.ep12335756 ↗
57. The cell cycle and cell death10.1016/0960-9822(93)90211-6 ↗
58. Signalling and chromatin fragmentation in thymocyte apoptosis10.1111/j.1600-065X.1994.tb00896.x ↗
59. Calcium-activated DNA fragmentation in rat liver nuclei10.1016/S0021-9258(18)83363-4 ↗
60. Antifungal activity of silver nanoparticles obtained by green synthesis10.1590/S0036-46652015000200011 ↗
61. Antifungal resistance of candidal biofilms formed on denture acrylic in vitro10.1177/00220345010800031101 ↗
62. The antifungal activity of graphene oxide-silver nanocomposites10.1016/j.biomaterials.2013.02.001 ↗
63. Antifungal activity of silver nanoparticles in combination with nystatin and chlorhexidine digluconate against Candida albicans and Candida glabrata biofilms10.1111/myc.12093 ↗
64. Antifungal applications of Ag-decorated hydroxyapatite nanoparticles10.1155/2013/174398 ↗
65. Bacterial adhesion of Streptococcus mutans to provisional fixed prosthodontic material10.1016/S0022-3913(07)60146-2 ↗
66. Antifungal activity and mode of action of silver nano-particles on Candida albicans10.1007/s10534-008-9159-2 ↗
67. A novel L1 retrotransposon marker for HeLa cell line identification10.2144/000113089 ↗
68. Predoi, D., Iconaru, S.L., and Predoi, M.V. (2018). Bioceramic Layers with Antifungal Properties. Coatings, 8.10.3390/coatings8080276 ↗
69. Antibacterial, cytotoxicity and physical properties of laser—Silver doped hydroxyapatite layers10.1016/j.msec.2012.12.018 ↗
70. Biomedical properties of laser prepared silver-doped hydroxyapatite10.1134/S1054660X11130159 ↗
71. Sola-Ruiz, M.F., Perez-Martinez, C., Labaig-Rueda, C., Carda, C., and Martín De Llano, J.J. (2017). Behavior of Human Osteoblast Cells Cultured on Titanium Discs in Relation to Surface Roughness and Presence of Melatonin. Int. J. Mol. Sci., 18.10.3390/ijms18040823 ↗
72. The effects of combined micron-/submicron-scale surface roughness and nanoscale features on cell proliferation and differentiation10.1016/j.biomaterials.2011.01.029 ↗
73. Effects of titanium surface roughness on mesenchymal stem cell commitment and differentiation signaling
74. Effect of various implant coatings on biological responses in MG63 using cDNA microarray10.1111/j.1365-2842.2005.01553.x ↗
75. Survival of hydroxyapatite-coated implants: A meta-analytic review10.1053/joms.2000.18269 ↗
76. Osteoblasts exhibit a more differentiated phenotype and increased bone morphogenetic protein production on titanium alloy substrates than on poly-ether-ether-ketone10.1016/j.spinee.2012.02.002 ↗
77. Effect of ultraviolet irradiation on the osseointegration of a titanium alloy with bone10.4103/ccd.ccd_576_17 ↗
78. Ultraviolet irradiation improves the hydrophilicity and osteo-conduction of hydroxyapatite10.1186/s13018-020-01949-3 ↗