Use of Silver Nanoparticle–Gelatin/Alginate Scaffold to Repair Skull Defects
Source abstract
Abstract
The major objectives of this study were to investigate the effects of silver nanoparticles– gelatin (AgNPs) on the physical and chemical properties of gelatin/alginate (Gel/Alg) scaffolds and the bone-promoting effect of AgNP–Gel/Alg scaffolds. Gel/Alg scaffolds consisting of 0 μM, 200 μM, 400 μM, and 600 μM AgNPs were prepared. SEM was used to evaluate the physical properties of the scaffolds. A CCK-8 assay was performed to determine the cell proliferation activity, and Micro-CT and histological analysis were used to assess the osteogenic effect. The pore size, porosity, and the water absorption and degradation rates of AgNP–Gel/Alg scaffolds were found to be increased compared with those of Gel/Alg scaffolds (control group). CCK-8 showed that cell proliferation activity in the 200 μM group was significantly higher than that in the control group. Micro-CT analysis showed that there was more new bone around AgNP–Gel/Alg than the control group, and the amount of bone formation in the 200 μM group was significantly higher than that in the other groups. Masson staining showed that numerous collagen fibers had proliferated around the AgNP–Gel/Alg scaffold and tended to thicken over time. AgNP–Gel/Alg scaffolds promoted the repair of skull defects in New Zealand rabbits and exerted a marked osteogenic effect in vivo. The 200 μM AgNP–Gel/Alg scaffold was shown to be more suitable for bone tissue engineering materials.
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Research context
OpenAlex topics: Bone Tissue Engineering Materials · Graphene and Nanomaterials Applications · Dental Implant Techniques and Outcomes
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Orthodontics, School of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang 110001, China
Funding
Liaoning province key research and development plan project · Shenyang Science and Technology Project
References (28)
Show source-provided references
1. Bone grafts and biomaterials substitutes for bone defect repair: A review
2. A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering: The unsolved challenge10.1302/2046-3758.73.BJR-2017-0270.R1 ↗
3. Vertebral Implantation of NELL-1 Enhances Bone Formation in an Osteoporotic Sheep Model10.1089/ten.tea.2015.0230 ↗
4. Bone substitutes in the Netherlands a systematic literature review10.1016/j.actbio.2010.07.035 ↗
5. Preparation and characterization of gelatin/α-TCP/SF biocomposite scaffold for bone tissue regeneration10.1016/j.ijbiomac.2017.09.030 ↗
6. Development of a nanocomposite scaffold of gelatin-alginate-graphene oxide for bone tissue engineering10.1016/j.ijbiomac.2019.04.113 ↗
7. Comparison of three-dimensional printing and vacuum freeze-dried techniques for fabricating composite scaffolds10.1016/j.bbrc.2016.07.050 ↗
8. Antibacterial Melamine Foams Decorated with in Situ Synthesized Silver Nanoparticles10.1021/acsami.8b01442 ↗
9. Silver nanoparticles, Synthesis methods, bio-applications and properties
10. Wypij, M., Jędrzejewski, T., Ostrowski, M., Trzcińska, J., Rai, J., and Golińska, P. (2020). Biogenic Silver Nanoparticles: Assessment of Their Cytotoxicity, Genotoxicity and Study of Capping Proteins. Moleciles, 25.10.3390/molecules25133022 ↗
11. Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing10.1002/cmdc.200900502 ↗
12. Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing10.1016/j.nano.2011.01.003 ↗
13. Silver nanoparticles promote osteogenic differentiation of human urine-derived stem cells at noncytotoxic concentrations10.2147/IJN.S59753 ↗
14. Silver nanoparticles promote osteogenesis of mesenchymal stem cells and improve bone fracture healing in osteogenesis mechanism mouse model10.1016/j.nano.2015.07.016 ↗
15. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog10.1111/jcmm.15158 ↗
16. Porous HA ceramic for bone replacement, role of the pores and interconnections—Experimental study in the rabbit10.1023/A:1011256107282 ↗
17. Viable osteogenic cells are obligatory for tissue-engineered ectopic bone formation in goats10.1089/107632703764664792 ↗
18. Three-dimensional macroporous calcium phosphate bioceramics with nested chitosan sponges for load-bearing bone implants10.1002/jbm.10176 ↗
19. Osteoconductive and Osteoinductive Biodegradable Microspheres Serving as Injectable Micro-scaffolds for Bone Regeneration
20. Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE10.1016/j.nano.2009.01.012 ↗
21. Bactericidal effects of silver nanoparticles on lactobacilli and the underlying mechanism10.1021/acsami.7b17274 ↗
22. Silver nanoparticles, Evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells10.1016/j.toxlet.2010.12.001 ↗
23. Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA-TAZ axis10.1002/cbin.11180 ↗
24. Studies on the biocompatibility and the interaction of silver nanoparticles with human mesenchymal stem cells (hMSCs)10.1007/s00423-009-0472-1 ↗
25. Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation10.30699/ijp.2020.101715.2013 ↗
26. Alginate/Gelatin scaffolds incorporated with Silibinin-loaded Chitosan nanoparticles for bone formation in vitro10.1016/j.colsurfb.2017.06.048 ↗
27. Stimulation of healing within a rabbit calvarial defect by a PCL/PLGA scaffold blended with TCP using solid freeform fabrication technology10.1007/s10856-012-4761-9 ↗
28. Bone formation on the apatite-coated zirconia porous scaffolds within a rabbit calvarial defect10.1177/0885328207078075 ↗