Incorporation of Ca, P, Mg, and Zn Elements in Ti-30Nb-5Mo Alloy by Micro-Arc Oxidation for Biomedical Implant Applications: Surface Characterization, Cellular Growth, and Microorganisms’ Activity
Source abstract
Abstract
Micro-arc oxidation (MAO) is a surface modification technique used to improve the surface properties of titanium alloys, such as corrosion, wear resistance, and osseointegration. In addition to promoting the growth of a porous oxide coating on the sample’s surface, it is also possible to incorporate bioactive elements into this coating, such as calcium, phosphorus, and magnesium, as well as elements with antimicrobial action, such as zinc. Thus, this study aimed at the surface modification of the β Ti-30Nb-5Mo alloy by the MAO method, incorporating calcium, phosphorus, magnesium, and zinc to improve osseointegration and promote bactericidal character in the produced coating. The results showed that the porosity, roughness, and crystallinity of the coating tend to increase with increasing Zn concentration in the electrolyte, while the contact angle decreases. The antimicrobial activity was promoted against the E. faecalis and P. aeruginosa bacteria strains and the C. albicans fungus. Incorporating Zn on the surface also did not negatively affect adiposetissue-derived mesenchymal stem cell differentiation, and promoted more significant growth of these cells on the samples’ surface.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Titanium Alloys Microstructure and Properties · Orthopaedic implants and arthroplasty
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratório de Anelasticidade e Biomateriais, UNESP—Universidade Estadual Paulista, Bauru 17033-360, SP, Brazil
Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche (ISM-CNR), Via del Fosso del Cavaliere 100, 00133 Rome, Italy
Istituto Zooprofilattico Sperimentale Lazio e Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, Italy
Dipartimento di Scienze, Università della Basilicata, Via dell’Ateneo Lucano 10, 85100 Potenza, Italy
Department of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119991 Moscow, Russia
Laboratory of Regenerative Orthopaedics, Research Unit of Orthopaedic, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Via Alvaro del Portillo 21, 00128 Rome, Italy
Operative Research Unit of Orthopaedics, Fondazione Policlinico Universitario Campus Bio-Medico, Via Alvaro del Portillo 200, 00128 Rome, Italy
Funding
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES) · Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
References (75)
Show source-provided references
1. Antibacterial and Microstructure Properties of Titanium Surfaces Modified with Ag-Incorporated Nanotube Arrays10.1590/1980-5373-MR-2015-0534 ↗
2. Shimabukuro, M. (2020). Antibacterial Property and Biocompatibility of Silver, Copper, and Zinc in Titanium Dioxide Layers Incorporated by One-Step Micro-Arc Oxidation: A Review. Antibiotics, 9.10.3390/antibiotics9100716 ↗
3. Treatment of Infections Associated with Surgical Implants10.1056/NEJMra035415 ↗
4. Komarova, E.G., Sharkeev, Y.P., Sedelnikova, M.B., Prosolov, K.A., Khlusov, I.A., Prymak, O., and Epple, M. (2020). Zn- or Cu-Containing CaP-Based Coatings Formed by Micro-arc Oxidation on Titanium and Ti-40Nb Alloy: Part I—Microstructure, Composition and Properties. Materials, 13.10.3390/ma13184116 ↗
5. Impressive strides in antibacterial performance amelioration of Ti-based implants via plasma electrolytic oxidation (PEO): A review of the recent advancements10.1016/j.cej.2022.136003 ↗
6. On the enhanced antibacterial activity of plasma electrolytic oxidation (PEO) coatings that incorporate particles: A review10.1016/j.ceramint.2020.05.206 ↗
7. Surface modification of valve metals using plasma electrolytic oxidation for antibacterial applications: A review10.1002/jbm.a.36259 ↗
8. Biofilms and Chronic Infections10.1001/jama.299.22.2682 ↗
9. New Ti-alloys and surface modifications to improve the mechanical properties and the biological response to orthopedic and dental implants: A review10.1155/2016/2908570 ↗
10. Study on Zn-doped antibacterial bioactive coatings on Ti6Al4V titanium alloy surfaces by micro-arc oxidation10.1016/j.surfcoat.2023.129724 ↗
11. Grigoriev, S., Sotova, C., Vereschaka, A., Uglov, V., and Cherenda, N. (2023). Modifying Coatings for Medical Implants Made of Titanium Alloys. Metals, 13.10.3390/met13040718 ↗
12. Surface engineering of titanium alloys for wear protection10.1243/1350650981542263 ↗
13. Influence of the heat treatment temperature on the MAO coating produced in the Ti-25Ta-25Zr alloy10.1016/j.jmrt.2023.08.212 ↗
14. Micro-abrasive wear behavior by ball cratering on MAO coating of Ti–25Ta alloy10.1016/j.jmrt.2023.08.023 ↗
15. Jiang, B.L., and Ge, Y.F. (2013). Corrosion Prevention of Magnesium Alloys, Woodhead Publishing.
16. Liu, S., Chen, J., Zhang, D., Wang, Y., He, Z., and Guo, P. (2022). Properties of Micro-Arc Oxidation Coatings on 5052 Al Alloy Sealed by SiO2 Nanoparticles. Coatings, 12.10.3390/coatings12030373 ↗
17. Research of growth mechanism of ceramic coatings fabricated by micro-arc oxidation on magnesium alloys at high current mode10.1016/j.jma.2015.07.003 ↗
18. Formation mechanism and adhesive strength of a hydroxyapatite/TiO2 composite coating on a titanium surface prepared by micro-arc oxidation10.1016/j.apsusc.2015.11.086 ↗
19. Effect of NaAlO2 concentrations on the properties of micro-arc oxidation coatings on pure titanium10.1016/j.matlet.2016.02.024 ↗
20. Review: The Surface Modification of Pure Titanium by Micro-Arc Oxidation (MAO) Process10.1088/1742-6596/1973/1/012114 ↗
21. Rossi, M.C., dos Santos, R.F., Kuroda, P.A.B., and Afonso, C.R.M. (2023). Characteristics of ceramic-like coatings obtained by plasma electrolyte oxidation on different Ti alloys. Boletín Soc. Española Cerámica Vidr.10.1016/j.bsecv.2023.04.002 ↗
22. Corrosion behaviors of bioactive element coatings on PEO-treated Ti-6Al-4V alloys10.1016/j.surfcoat.2018.07.093 ↗
23. Effects of silver concentrations on microstructure and properties of nanostructured titania films10.1016/j.matdes.2014.09.060 ↗
24. Antibacterial activity of single crystalline silver-doped anatase TiO2 nanowire arrays10.1016/j.apsusc.2015.12.094 ↗
25. The Cu-containing TiO2 coatings with modulatory effects on macrophage polarization and bactericidal capacity prepared by micro-arc oxidation on titanium substrates10.1016/j.colsurfb.2018.06.020 ↗
26. Activating macrophages for enhanced osteogenic and bactericidal performance by Cu ion release from micro/nano-topographical coating on a titanium substrate10.1016/j.actbio.2019.09.030 ↗
27. Fosca, M., Streza, A., Antoniac, I.V., Vadalà, G., and Rau, J.V. (2023). Ion-Doped Calcium Phosphate-Based Coatings with Antibacterial Properties. J. Funct. Biomater., 14.10.3390/jfb14050250 ↗
28. Corrosion behavior of Zn-incorporated antibacterial TiO2 porous coating on titanium10.1016/j.ceramint.2016.07.220 ↗
29. Effects of surface roughening on antibacterial and osteogenic properties of Ti-Cu alloys with different Cu contents10.1016/j.jmst.2021.01.067 ↗
30. Fadeeva, I.V., Deyneko, D.V., Knotko, A.V., Olkhov, A.A., Slukin, P.V., Davydova, G.A., Trubitsyna, T.A., Preobrazhenskiy, I.I., Gosteva, A.N., and Antoniac, I.V. (2023). Antibacterial Composite Material Based on Polyhydroxybutyrate and Zn-Doped Brushite Cement. Polymers, 15.10.3390/polym15092106 ↗
31. Improved cytocompatibility and antibacterial properties of zinc-substituted brushite bone cement based on β-tricalcium phosphate10.1007/s10856-021-06575-x ↗
32. ROS induced bactericidal activity of amorphous Zn-doped titanium oxide coatings and enhanced osseointegration in bacteria-infected rat tibias10.1016/j.actbio.2020.02.036 ↗
33. Biological Responses and Mechanisms of Human Bone Marrow Mesenchymal Stem Cells to Zn and Mg Biomaterials10.1021/acsami.7b06654 ↗
34. Is there a potential therapeutic value of copper and zinc for osteoporosis?10.1079/PNS2002154 ↗
35. Preparation and characterization of novel as-cast Ti-Mo-Nb alloys for biomedical applications10.1038/s41598-022-14820-8 ↗
36. Effect of Thermomechanical Treatments on Microstructure, Phase Composition, Vickers Microhardness, and Young’s Modulus of Ti-xNb-5Mo Alloys for Biomedical Applications10.3390/met12050788 ↗
37. Influence of Nb addition on the structure, microstructure, Vickers microhardness, and Young’s modulus of new β Ti-xNb-5Mo alloys system10.1016/j.jmrt.2023.06.168 ↗
38. High-strength β stabilized Ti-Nb-Fe-Cr alloys with large plasticity10.1016/j.msea.2018.07.031 ↗
39. Corrosion behaviour and biocompatibility evaluation of low modulus Ti–16Nb shape memory alloy as potential biomaterial10.1016/j.matlet.2009.02.062 ↗
40. Study of the effect of ZnO nanoparticles addition to PEO coatings on pure titanium substrate: Microstructural analysis, antibacterial effect and corrosion behavior of coatings in Ringer’s physiological solution10.1016/j.jallcom.2017.12.366 ↗
41. Characterization and property of dual-functional Zn-incorporated TiO2 micro-arc oxidation coatings: The influence of current density10.1016/j.jallcom.2019.151893 ↗
42. Synthesis and characterization of a bi-functional hydroxyapatite/Cu-doped TiO2 composite coating10.1016/j.ceramint.2018.12.158 ↗
43. Review of micro-arc oxidation of titanium alloys: Mechanism, properties and applications10.1016/j.jallcom.2023.169773 ↗
44. Development of novel Ti-Mo-Mn alloys for biomedical applications10.1038/s41598-020-62865-4 ↗
45. Micro-arc oxidation of β-titanium alloy: Structural characterization and osteoblast compatibility10.1016/j.surfcoat.2009.06.043 ↗
46. Characterization and property of bifunctional Zn-incorporated TiO2 micro-arc oxidation coatings: The influence of different Zn sources10.1016/j.ceramint.2019.06.228 ↗
47. Kuroda, P.A.B., Grandini, C.R., and Afonso, C.R.M. (2023). Surface Characterization of New β Ti-25Ta-Zr-Nb Alloys Modified by Micro-Arc Oxidation. Materials, 16.10.3390/ma16062352 ↗
48. Theoretical Calculation of the Local Heating Effect on the Crystallization of TiO2 Prepared by Sparking Anodization10.2174/1573413711666150212234928 ↗
49. Chen, K.-T., Huang, J.-W., Lin, W.-T., Kuo, T.-Y., Chien, C.-S., Chang, C.-P., and Lin, Y.-D. (2023). Effects of Micro-Arc Oxidation Discharge Parameters on Formation and Biomedical Properties of Hydroxyapatite-Containing Flower-like Structure Coatings. Materials, 16.10.3390/ma16010057 ↗
50. Oxide ceramic coatings with amorphous/nano-crystalline dual-structures prepared by micro-arc oxidation on Ti–Nb–Zr medium entropy alloy surfaces for biomedical applications10.1016/j.ceramint.2023.02.180 ↗
51. Morphology, Crystalline Structure and Chemical Composition Of MAO Treated Ti-15Zr-Mo Surfaces Enriched with Bioactive Ions10.1590/1980-5373-mr-2019-0005 ↗
52. Assessment of applied voltage on the structure, pore size, hardness, elastic modulus, and adhesion of anodic coatings in Ca-, P-, and Mg-rich produced by MAO in Ti-25Ta-Zr alloys10.1016/j.jmrt.2023.08.165 ↗
53. Kumari, P., Saha, R., Saikia, G., Bhujel, A., Choudhury, M.G., Jagdale, P., and Paul, S. (2023). Synthesis of Mixed-Phase TiO2–ZrO2 Nanocomposite for Photocatalytic Wastewater Treatment. Toxics, 11.10.3390/toxics11030234 ↗
54. Investigating the optimum conditions for the formation of calcium oxide, used for CO2 sequestration, by thermal decomposition of calcium acetate10.1088/1742-6596/371/1/012075 ↗
55. Dual-electrolyte fabrication of micro arc oxidation coatings on Ta–12W alloy with enhanced wear resistance10.1016/j.vacuum.2022.111698 ↗
56. Skorupska, M., Kamedulski, P., Lukaszewicz, J.P., and Ilnicka, A. (2021). The Improvement of Energy Storage Performance by Sucrose-Derived Carbon Foams via Incorporating Nitrogen Atoms. Nanomaterials, 11.10.3390/nano11030760 ↗
57. Electrochemical modification of the Ti-15Mo alloy surface in solutions containing ZnO and Zn3(PO4)2 particles10.1016/j.msec.2020.111098 ↗
58. Advancement of plasma electrolytic oxidation towards non-valve metals10.1016/j.surfcoat.2022.128403 ↗
59. Influence of the molybdenum on characteristics of oxide films produced by micro-arc oxidation on Ti-15Zr-based alloys10.1016/j.surfcoat.2021.126856 ↗
60. Fabrication and application of superhydrophilic surfaces: A review10.1080/01694243.2012.697714 ↗
61. Study on strontium doped bioactive coatings on titanium alloys surfaces by micro-arc oxidation10.1016/j.surfcoat.2022.129045 ↗
62. Komarova, E.G., Sharkeev, Y.P., Sedelnikova, M.B., Prymak, O., Epple, M., Litvinova, L.S., Shupletsova, V.V., Malashchenko, V.V., Yurova, K.A., and Dzyuman, A.N. (2020). Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance. Materials, 13.10.3390/ma13194366 ↗
63. Effect of oxidation time on cytocompatibility of ultrafine-grained pure Ti in micro-arc oxidation treatment10.1016/j.surfcoat.2018.02.044 ↗
64. Incorporation of silver and strontium in hydroxyapatite coating on titanium surface for enhanced antibacterial and biological properties10.1016/j.msec.2016.10.079 ↗
65. Characterization and in vitro properties of anti-bacterial Ag-based bioceramic coatings formed on zirconium by micro arc oxidation and thermal evaporation10.1016/j.surfcoat.2017.10.023 ↗
66. Improving surface features of PEO coatings on titanium and titanium alloys with zirconia particles: A review10.1016/j.surfin.2020.100888 ↗
67. Effect of ZrO2 nanoparticles addition to PEO coatings on Ti–6Al–4V substrate: Microstructural analysis, corrosion behavior and antibacterial effect of coatings in Hank’s physiological solution10.1016/j.ceramint.2020.02.084 ↗
68. Antibacterial Properties of Metallic Elements for Alloying Evaluated with Application of JIS Z 2801:200010.2355/isijinternational.48.1299 ↗
69. Evaluation of osteogenic and antibacterial properties of strontium/silver-containing porous TiO2 coatings prepared by micro-arc oxidation10.1002/jbm.b.34719 ↗
70. Structure, MC3T3-E1 Cell Response, and Osseointegration of Macroporous Titanium Implants Covered by a Bioactive Microarc Oxidation Coating with Microporous Structure10.1021/am405680d ↗
71. Antibacterial ability and osteogenic activity of porous Sr/Ag-containing TiO2 coatings10.1088/1748-6041/11/4/045008 ↗
72. Osteoconductivity of hydrophilic microstructured titanium implants with phosphate ion chemistry10.1016/j.actbio.2009.02.026 ↗
73. Enhancing surface free energy and hydrophilicity through chemical modification of microstructured titanium implant surfaces10.1002/jbm.a.30518 ↗
74. Enhanced Antibacterial Activity of Nanocrystalline ZnO Due to Increased ROS-Mediated Cell Injury10.1002/adfm.200801081 ↗
75. Cellular response to zinc-containing organoapatite: An in vitro study of proliferation, alkaline phosphatase activity and biomineralization10.1016/j.biomaterials.2005.01.043 ↗