journal article·2022-04-06Open access

Assessing the Use of Aloe vera Gel Alone and in Combination with Lemongrass Essential Oil as a Coating Material for Strawberry Fruits: HPLC and EDX Analyses

Hanaa S. Hassan, Mervat EL-Hefny, Ibrahim M. Ghoneim, Mina S. R. Abd El-Lahot, Mohammad Akrami, Asma A. Al-Huqail, Hayssam M. Ali, Doaa Y. Abd-Elkader

Coatings· Volume 12· Issue 4

Abstract

Strawberry is a non-climacteric fruit but exhibits a limited postharvest life due to rapid softening and decay. A strawberry coating that is natural and safe for human consumption can be used to improve the appearance and safeguard the fruits. In this study, 20% and 40% Aloe vera gel alone or in combination with 1% lemongrass essential oil (EO) was used as an edible coating for strawberries. After application of all the treatments, the strawberry fruits were stored at a temperature of 5 ± 1 °C at a relative humidity (RH) of 90%–95% for up to 16 days and all the parameters were analyzed and compared to control (uncoated fruits). The results show that A. vera gel alone or with lemongrass EO reduced the deterioration and increased the shelf life of the fruit. Treatment with A. vera gel and lemongrass EO decreased acidity and total anthocyanins and maintained fruit firmness. Treatment with A. vera gel 40% + lemongrass EO 1% led to the lowest weight loss, retained firmness and acidity, but increased the total soluble solids and total anthocyanins compared to uncoated fruits during storage of up to 16 days. The phenolic compounds of A. vera gel were analyzed by HPLC, and the most abundant compounds were found to be caffeic (30.77 mg/mL), coumaric (22.4 mg/mL), syringic (15.12 mg/mL), sinapic (14.05 mg/mL), ferulic (8.22 mg/mL), and cinnamic acids (7.14 mg/mL). Lemongrass EO was analyzed by GC–MS, and the most abundant compounds were identified as α-citral (neral) (40.10%) ꞵ-citral (geranial) (30.71%), γ-dodecalactone (10.24%), isoneral (6.67%), neryl acetal (5.64%), and linalool (1.77%). When the fruits were treated with 20% or 40% A. vera gel along with 1% lemongrass, their total phenolic content was maintained during the storage period (from 4 to 8 days). The antioxidant activity was relatively stable during the 8 days of cold storage of the fruits coated with A. vera gel combined with lemongrass EO because the activity of both 20% and 40% gel was greater than that for the other treatments after 12 days of storage in both experiments. Moreover, all the treatments resulted in lower numbers of total microbes at the end of the storage period compared with the control treatment. This study indicates that the use of Aloe vera gel with lemongrass EO as an edible coating considerably enhances the productivity of strawberry fruits and the treatment could be used on a commercial scale.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Essential Oils and Antimicrobial Activity · Phytochemicals and Antioxidant Activities · Postharvest Quality and Shelf Life Management

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Vegetable, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt

Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt

Department of Food Science & Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt

Department of Engineering, University of Exeter, Exeter EX4 4QF, UK

Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2455, Riyadh 11451, Saudi Arabia

Funding

Researchers Supporting Project King Saud University, Riyadh, Saudi Arabia

References (95)

Show source-provided references
1. Ahn, M.G., Kim, D.S., Ahn, S.R., Sim, H.S., Kim, S., and Kim, S.K. (2021). Characteristics and Trends of Strawberry Cultivars throughout the Cultivation Season in a Greenhouse. Horticulturae, 7.10.3390/horticulturae7020030 ↗
2. Ilari, A., Toscano, G., Boakye-Yiadom, K.A., Duca, D., and Foppa Pedretti, E. (2021). Life Cycle Assessment of Protected Strawberry Productions in Central Italy. Sustainability, 13.10.3390/su13094879 ↗
3. Positive responses of strawberry (Fragaria × ananassa Duch.) explants to salicylic and iron nanoparticle application under salinity conditions10.1007/s11240-018-1420-y ↗
4. Extending the Shelf Life of Strawberries by the Sonochemical Coating of their Surface with Nanoparticles of an Edible Anti-Bacterial Compound10.3390/applnano2010002 ↗
5. Organic production of strawberry: A review
6. Eum, H.-L., Han, S.-H., and Lee, E.-J. (2021). High-CO2 Treatment Prolongs the Postharvest Shelf Life of Strawberry Fruits by Reducing Decay and Cell Wall Degradation. Foods, 10.10.3390/foods10071649 ↗
7. Chiabrando, V., Garavaglia, L., and Giacalone, G. (2019). The Postharvest Quality of Fresh Sweet Cherries and Strawberries with an Active Packaging System. Foods, 8.10.3390/foods8080335 ↗
8. Improving the shelf-life and quality of fresh and minimally-processed fruits and vegetables for a modern food industry: A comprehensive critical review from the traditional technologies into the most promising advancements10.1080/10408398.2018.1553025 ↗
9. Ziv, C., and Fallik, E. (2021). Postharvest Storage Techniques and Quality Evaluation of Fruits and Vegetables for Reducing Food Loss. Agronomy, 11.10.3390/agronomy11061133 ↗
10. Agriopoulou, S., Stamatelopoulou, E., Sachadyn-Król, M., and Varzakas, T. (2020). Lactic Acid Bacteria as Antibacterial Agents to Extend the Shelf Life of Fresh and Minimally Processed Fruits and Vegetables: Quality and Safety Aspects. Microorganisms, 8.10.3390/microorganisms8060952 ↗
11. Chitosan, a Biopolymer With Triple Action on Postharvest Decay of Fruit and Vegetables: Eliciting, Antimicrobial and Film-Forming Properties10.3389/fmicb.2018.02745 ↗
12. Tzortzakis, N., Xylia, P., and Chrysargyris, A. (2019). Sage Essential Oil Improves the Effectiveness of Aloe vera Gel on Postharvest Quality of Tomato Fruit. Agronomy, 9.10.3390/agronomy9100635 ↗
13. Abd-Elkader, D.Y., Salem, M.Z.M., Komeil, D.A., Al-Huqail, A.A., Ali, H.M., Salah, A.H., Akrami, M., and Hassan, H.S. (2021). Post-Harvest Enhancing and Botrytis cinerea Control of Strawberry Fruits Using Low Cost and Eco-Friendly Natural Oils. Agronomy, 11.10.3390/agronomy11061246 ↗
14. Farina, V., Passafiume, R., Tinebra, I., Palazzolo, E., and Sortino, G. (2020). Use of Aloe vera Gel-Based Edible Coating with Natural Anti-Browning and Anti-Oxidant Additives to Improve Post-Harvest Quality of Fresh-Cut ‘Fuji’ Apple. Agronomy, 10.10.1155/2020/8303140 ↗
15. Ghoora, M.D., and Srividya, N. (2020). Effect of Packaging and Coating Technique on Postharvest Quality and Shelf Life of Raphanus sativus L. and Hibiscus sabdariffa L. Microgreens. Foods, 9.10.3390/foods9050653 ↗
16. Effect of chitosan-based coatings enriched with savory and/or tarragon essential oils on postharvest maintenance of kumquat (Fortunella sp.) fruit10.1002/fsn3.835 ↗
17. Chitosan films and coatings containing essential oils: The antioxidant and antimicrobial activity, and application in food systems10.1016/j.foodres.2016.10.004 ↗
18. Effect of edible coatings on the shelf-life of fresh strawberries: A comparative study using TOPSIS-Shannon entropy method10.1016/j.nfs.2021.02.003 ↗
19. Effects of lemongrass oil application and modified atmosphere packaging on the postharvest life and quality of strawberry fruits10.1016/j.scienta.2019.05.054 ↗
20. Enhancing the functionality of chitosan- and alginate-based active edible coatings/films for the preservation of fruits and vegetables: A review10.1016/j.ijbiomac.2020.07.083 ↗
21. Optimized irrigation regime and planting technique improve yields and economics in aloe vera [Aloe barbadensis (Miller)]10.1016/j.indcrop.2021.113539 ↗
22. Screening methods used to determine the anti-microbial properties of Aloe vera inner gel10.1016/j.ymeth.2007.03.004 ↗
23. Characterisation of gels from different Aloe spp. as antifungal treatment: Potential crops for industrial applications10.1016/j.indcrop.2012.06.002 ↗
24. Factors Affecting the Moisture Permeability of Lipid-Based Edible Films: A Review10.1080/10408690290825466 ↗
25. The therapeutic properties and applications of Aloe vera: A review10.1016/j.hermed.2018.01.002 ↗
26. Aloe vera gel: An update on its use as a functional edible coating to preserve fruits and vegetables10.1016/j.porgcoat.2020.106007 ↗
27. Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit10.1016/j.postharvbio.2015.11.019 ↗
28. Potential of Aloe vera gel coating for storage life extension and quality conservation of fruits and vegetables: An overview10.1111/jfbc.13640 ↗
29. Effects of Aloe vera gel and MAP on bioactive compounds and quality attributes of cherry laurel fruit during cold storage10.1016/j.scienta.2019.01.030 ↗
30. Aloe vera gel coating delays postharvest browning and maintains quality of harvested litchi fruit10.1016/j.postharvbio.2019.110960 ↗
31. Aloe vera gel coating for post-harvest quality maintenance of fresh fig fruits
32. Use of essential oils for prolonging postharvest life of fresh fruits and vegetables
33. Effects of Essential Oil Vapour Treatment on the Postharvest Disease Control and Different Defence Responses in Two Mango (Mangifera indica L.) Cultivars10.1007/s11947-017-1891-6 ↗
34. Potential Insecticidal Activity of Four Essential Oils against the Rice Weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)10.15376/biores.16.4.7767-7783 ↗
35. Moustafa, M.A.M., Awad, M., Amer, A., Hassan, N.N., Ibrahim, E.-D.S., Ali, H.M., Akrami, M., and Salem, M.Z.M. (2021). Insecticidal Activity of Lemongrass Essential Oil as an Eco-Friendly Agent against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae). Insects, 12.10.3390/insects12080737 ↗
36. Mansour, M.M.A., El-Hefny, M., Salem, M.Z.M., and Ali, H.M. (2020). The Biofungicide Activity of Some Plant Essential Oils for the Cleaner Production of Model Linen Fibers Similar to Those Used in Ancient Egyptian Mummification. Processes, 8.10.3390/pr8010079 ↗
37. Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (Lemon grass)10.4103/2231-4040.79796 ↗
38. Utama, I.M.S., Yulianti, N.L., Prastya, O.A., and Luther, G. (2014, January 6–9). Sesame and Lemon Grass Oils as Coating Materials to Reduce the Deterioration of Tomato Fruits during Storage. Proceedings of the Indonesian Horticultural Society National Seminar, Malang, Indonesia.
39. Antimicrobial Activity of Some Essential Oils Against Microorganisms Deteriorating Fruit Juices10.4489/MYCO.2006.34.4.219 ↗
40. Alamgir, A.N.M. (2018). Secondary Metabolites: Secondary Metabolic Products Consisting of C and H; C, H, and O; N, S, and P Elements; and O/N Heterocycles. Therapeutic Use of Medicinal Plants and Their Extracts: Volume 2: Phytochemistry and Bioactive Compounds, Springer International Publishing.10.1007/978-3-319-92387-1_3 ↗
41. Impact of gelatin, lemongrass oil and peppermint oil on storability and fruit quality of Samany date palm under cold storage10.1186/s42269-019-0255-y ↗
42. Essential Oils of Lemongrass Cymbopogon citratus Stapf Induces Apoptosis and Cell Cycle Arrest in A549 Lung Cancer Cells10.1155/2020/5924856 ↗
43. Color and composition of beauty products formulated with lemongrass essential oil: Cosmetics formulation with lemongrass essential oil10.1515/chem-2021-0066 ↗
44. Winterstar™ (‘FL 05-107’) Strawberry10.21273/HORTSCI.47.2.296 ↗
45. Immunomodulatory potential of acemannan (polysaccharide from Aloe vera) against radiation induced mortality in Swiss albino mice10.1080/09540105.2015.1079594 ↗
46. El-Hefny, M., Abo Elgat, W.A.A., Al-Huqail, A.A., and Ali, H.M. (2019). Essential and Recovery Oils from Matricaria chamomilla Flowers as Environmentally Friendly Fungicides Against Four Fungi Isolated from Cultural Heritage Objects. Processes, 7.10.3390/pr7110809 ↗
47. Okla, M.K., Alamri, S.A., Salem, M.Z.M., Ali, H.M., Behiry, S.I., Nasser, R.A., Alaraidh, I.A., Al-Ghtani, S.M., and Soufan, W. (2019). Yield, Phytochemical Constituents, and Antibacterial Activity of Essential Oils from the Leaves/Twigs, Branches, Branch Wood, and Branch Bark of Sour Orange (Citrus aurantium L.). Processes, 7.10.3390/pr7060363 ↗
48. Shehata, S.A., Abdeldaym, E.A., Ali, M.R., Mohamed, R.M., Bob, R.I., and Abdelgawad, K.F. (2020). Effect of Some Citrus Essential Oils on Post-Harvest Shelf Life and Physicochemical Quality of Strawberries during Cold Storage. Agronomy, 10.10.3390/agronomy10101466 ↗
49. Instrumental color and sensory acceptance of soy-based emulsions: A response surface approach10.1590/S0101-20612010000400039 ↗
50. The Association of Official Analytical Chemists (A.O.A.C.) (2000). Official Methods of Analysis, The Association of Official Analytical Chemists. [17th ed.].
51. Quantitative Methods for Anthocyanins10.1111/j.1365-2621.1968.tb00887.x ↗
52. Antibacterial activities of the phytochemicals-characterized extracts of Callistemon viminalis, Eucalyptus camaldulensis and Conyza dioscoridis against the growth of some phytopathogenic bacteria10.1016/j.micpath.2017.11.004 ↗
53. Effects of Extraction and Processing Methods on Antioxidant Compound Contents and Radical Scavenging Activities of Laver (Porphyra tenera)10.3746/pnf.2014.19.1.040 ↗
54. Hassan, H.S., Mohamed, A.A., Feleafel, M.N., Salem, M.Z.M., Ali, H.M., Akrami, M., and Abd-Elkader, D.Y. (2021). Natural Plant Extracts and Microbial Antagonists to Control Fungal Pathogens and Improve the Productivity of Zucchini (Cucurbita pepo L.) In Vitro and in Greenhouse. Horticulturae, 7.10.3390/horticulturae7110470 ↗
55. Moringa oleifera seeds-removed ripened pods as alternative for papersheet production: Antimicrobial activity and their phytoconstituents profile using HPLC10.1038/s41598-021-98415-9 ↗
56. How to optimize the drop plate method for enumerating bacteria10.1016/S0167-7012(00)00241-4 ↗
57. Snedecor, W., and Cochran, G. (1989). Statistical Methods, Iowa State University Press. [8th ed.].
58. Phenolic Constituents, Antioxidant and Preliminary Antimycoplasmic Activities of Leaf Skin and Flowers of Aloe vera (L.) Burm. f. (syn. A. barbadensis Mill.) from the Canary Islands (Spain)10.3390/molecules18054942 ↗
59. Aloe vera gel as a functional ingredient and natural preservative in mango nectar
60. Identification of Flavonoids and Phenolic Compound in Aloe vera gel by HPLC10.25130/tjps.v23i6.677 ↗
61. Chemical composition, antibacterial and antioxidant activities of lemongrass (Cymbopogon citratus) essential oil and its fractions obtained by vacuum distillation10.1088/1757-899X/1166/1/012051 ↗
62. Evaluation of essential oil yield and chemical composition of eight lemongrass (Cymbopogon spp.) cultivars under Himalayan region10.1080/10412905.2015.1014936 ↗
63. Volatile Profiling and Essential Oil Yield of Cymbopogon citratus (DC.) Stapf Treated with Rhizosphere Fungi and Some Important Fertilizers10.1080/0972060X.2019.1613933 ↗
64. Chemical composition and citral content in lemongrass (Cymbopogon citratus) essential oil at three maturity stages10.5897/AJB11.2939 ↗
65. Rehman, M.A., Asi, M.R., Hameed, A., and Bourquin, L.D. (2020). Effect of Postharvest Application of Aloe vera Gel on Shelf Life, Activities of Anti-Oxidative Enzymes, and Quality of ‘Gola’ Guava Fruit. Foods, 9.10.3390/foods9101361 ↗
66. Aloe vera gel enriched with garlic essential oil effectively controls anthracnose disease and maintains postharvest quality of banana fruit during storage10.1007/s13580-019-00159-z ↗
67. Antifungal efficacy of Aloe vera in vitro and its use as a preharvest treatment to maintain postharvest table grape quality10.1016/j.postharvbio.2010.04.006 ↗
68. Postharvest sour cherry quality and safety maintenance by exposure to Hot- water or treatment with fresh Aloe vera gel10.1007/s13197-012-0767-z ↗
69. Evaluation of Aloe vera Gel as an Alternative Edible Coating for Peach Fruits During Cold Storage Period10.1007/s10343-017-0397-5 ↗
70. Increasing shelf life of strawberries (Fragaria ssp) by using a banana starch-chitosan-Aloe vera gel composite edible coating10.1111/ijfs.14254 ↗
71. Impact of Aloe vera gel coating enriched with basil (Ocimum basilicum L.) essential oil on postharvest quality of strawberry fruit10.1007/s11694-020-00634-7 ↗
72. Development of a cactus-mucilage edible coating (Opuntia ficus indica) and its application to extend strawberry (Fragaria ananassa) shelf-life10.1016/j.foodchem.2004.07.002 ↗
73. Effect of Methylcellulose-Based Edible Coating on Strawberry Fruit’s Quality Maintenance During Storage10.1111/jfpp.12227 ↗
74. Improving strawberry fruit storability by edible coating as a carrier of thymol or calcium chloride
75. Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan10.1016/j.postharvbio.2013.06.008 ↗
76. Efficacy of limonene nano coatings on post-harvest shelf life of strawberries10.1016/j.lwt.2018.06.038 ↗
77. Effect of Aloe vera gel coating on antioxidant capacity, antioxidant enzyme activities and decay in raspberry fruit10.1016/j.lwt.2014.07.049 ↗
78. Lemongrass essential oil incorporated into alginate-based edible coating for shelf-life extension and quality retention of fresh-cut pineapple10.1016/j.postharvbio.2013.09.004 ↗
79. Antioxidant and Cellular Activities of Anthocyanins and Their Corresponding Vitisins A Studies in Platelets, Monocytes, and Human Endothelial Cells10.1021/jf035360v ↗
80. Impact of ultraviolet radiation treatments on the physicochemical properties, antioxidants, enzyme activity and microbial load in freshly prepared hand pressed strawberry juice10.1177/1082013214536708 ↗
81. Chitosan–Aloe vera gel coating delays postharvest decay of mango fruit10.1007/s13580-019-00224-7 ↗
82. Pre-harvest application of chitosan and postharvest Aloe vera gel coating enhances quality of table grape (Vitis vinifera L. cv. ‘Yaghouti’) during postharvest period10.1016/j.foodchem.2021.129012 ↗
83. Free radical-scavenging activity of Aloe vera (Aloe barbadensis Miller) extracts by supercritical carbon dioxide extraction10.1016/j.foodchem.2004.05.052 ↗
84. Comparative Study on Antioxidant Activities, Total Phenolic Compound and Pigment Contents of Tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca Extracted in Different Solvents Polarity10.1051/e3sconf/202014703012 ↗
85. Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines, 5.10.3390/medicines5030093 ↗
86. Potential Impact of Strawberries on Human Health: A Review of the Science10.1080/10408690490263756 ↗
87. Flavonoid Compounds in the Strawberry Fruit10.1111/j.1365-2621.1968.tb01367.x ↗
88. Foliar Spray with 24-Epibrassinolide Enhanced Strawberry Fruit Quality, Phytochemical Content, and Postharvest Life10.1007/s00344-019-10033-y ↗
89. The synergistic effects of Aloe vera gel and modified atmosphere packaging on the quality of strawberry fruit10.1111/jfpp.16003 ↗
90. Arrubla Vélez, J.P., Guerrero Álvarez, G.E., Vargas Soto, M.C., Cardona Hurtado, N., Pinzón, M.I., and Villa, C.C. (2021). Aloe Vera Gel Edible Coating for Shelf Life and Antioxidant Proprieties Preservation of Andean Blackberry. Processes, 9.10.3390/pr9060999 ↗
91. Solís-Contreras, G.A., Rodríguez-Guillermo, M.C., de la Luz Reyes-Vega, M., Aguilar, C.N., Rebolloso-Padilla, O.N., Corona-Flores, J., de Abril Alexandra Soriano-Melgar, L., and Ruelas-Chacon, X. (2021). Extending Shelf-Life and Quality of Minimally Processed Golden Delicious Apples with Three Bioactive Coatings Combined with Cinnamon Essential Oil. Foods, 10.10.3390/foods10030597 ↗
92. Postharvest sweet cherry quality and safety maintenance by Aloe vera treatment: A new edible coating10.1016/j.postharvbio.2005.09.006 ↗
93. Inhibitory effect of salicylic acid and Aloe vera gel edible coating on microbial load and chilling injury of orange fruit10.1016/j.scienta.2018.12.004 ↗
94. Antara, N.S., Paramita, D., Duwipayana, A.A., and Gunam, I. (2013, January 26–29). Inhibitory activity of lemongrass essential oil against Eschericia coli, Staphylococcus aureus, and Vibrio cholera. Proceedings of the Seminar Nasional Patpi 2013, Jember, Indonesia.
95. Radical scavenging activity prediction of o-coumaric acid thioamide10.1016/j.theochem.2007.06.034 ↗