Graphene Oxide Reinforcing Genipin Crosslinked Chitosan-Gelatin Blend Films
Source abstract
Abstract
This study was targeted towards the synthesis and characterization of new chitosan–gelatin biocomposite films reinforced with graphene oxide and crosslinked with genipin. The composites’ mode of structuration was characterized by Fourier Transform Infrared spectroscopy and X-ray diffraction, while morphology and topography were investigated by scanning electron microscopy, nano-computer tomography and profilometry. Eventually, thermal stability was evaluated through thermogravimetrical analysis, mechanical properties assessment was carried out to detect potential improvements as a result of graphene oxide (GO) addition and in vitro enzyme degradation was performed to discern the most promising formulations for the maturation of the study towards in vivo assays. In accordance with similar works, results indicated the possibility of using GO as an agent for adjusting films’ roughness, chemical stability and polymer structuration. The enzymatic stability of chitosan–gelatin (CHT-GEL) films was also improved by genipin (GEN) crosslinking and GO supplementation, with the best results being obtained for CHT-GEL-GEN and CHT-GEL-GEN-GO3 (crosslinked formulation with 3 wt.% GO). Yet, contrary to previous reports, no great enhancement of CHT-GEN-GEL-GO thermal performances was obtained by the incorporation of GO.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Graphene and Nanomaterials Applications · Electrospun Nanofibers in Biomedical Applications · Nanocomposite Films for Food Packaging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Medical Engineering, University Politehnica of Bucharest, Gh Polizu 1-7, 011061 Bucharest, Romania
Advanced Polymer Materials Group, University Politehnica of Bucharest, Gh Polizu 1-7, 011061 Bucharest, Romania
Faculty of Applied Chemistry and Materials Sciences, University Politehnica of Bucharest, Gh Polizu 1-7, 011061 Bucharest, Romania
Funding
National Authority for Scientific Research and Innovation, Operational Program Competitiveness Axis 1-Section E Program cofinanced from European Regional Development Fund “Investments for Your Future”
References (50)
Show source-provided references
1. Polymeric biomaterials in tissue engineering10.1203/01.pdr.0000305937.26105.e7 ↗
2. Dinescu, S., Ionita, M., Ignat, S.-R., Costache, M., and Hermenean, A. (2019). Graphene oxide enhances chitosan-based 3D scaffold properties for bone tissue engineering. Int. J. Mol. Sci., 20.10.3390/ijms20205077 ↗
3. Bherwani, A., Chang, C.-C., Pelled, G., Gazit, Z., Gazit, D., Rafailovich, M., and Simon, M. (2019). The influence of polymer blends on regulating chondrogenesis. Coatings, 9.10.3390/coatings9070451 ↗
4. In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering
5. Biocompatibility assessment of novel collagen-sericin scaffolds improved with hyaluronic acid and chondroitin sulfate for cartilage regeneration10.1155/2013/598056 ↗
6. Sodium alginate/graphene oxide composite films with enhanced thermal and mechanical properties10.1016/j.carbpol.2013.01.065 ↗
7. Design of cellulose–alginate films using PEG/NaOH aqueous solution as co-solvent10.1007/s10570-017-1412-9 ↗
8. Bioinspired 3D printable pectin-nanocellulose ink formulations10.1016/j.carbpol.2019.05.026 ↗
9. Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration10.1038/s41598-018-25643-x ↗
10. Polymeric biomaterials10.1016/S1359-6454(99)00299-2 ↗
11. Chitin and chitosan: Properties and applications10.1016/j.progpolymsci.2006.06.001 ↗
12. Biomedical applications of collagen10.1016/S0378-5173(01)00691-3 ↗
13. Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by a combined freeze-drying/leaching method10.1016/j.msec.2013.05.039 ↗
14. Preparation and evaluation of ciprofloxacin loaded chitosan-gelatin composite films for wound healing activity10.5138/ijdd.2010.0975.0215.02027 ↗
15. Development of polyelectrolyte chitosan-gelatin hydrogels for skin bioprinting10.1016/j.procir.2015.09.002 ↗
16. Preparation and characterization of chitosan-gelatin/glutaraldehyde scaffolds10.1080/00222348.2013.837290 ↗
17. Bioprinted chitosan-gelatin thermosensitive hydrogels using an inexpensive 3D printer10.1088/1758-5090/aa96dd ↗
18. Chitosan-gelatin porous scaffold incorporated with Chitosan nanoparticles for growth factor delivery in tissue engineering10.1016/j.carbpol.2018.07.023 ↗
19. Molecular interactions in gelatin/chitosan composite films10.1016/j.foodchem.2017.05.045 ↗
20. Study on genipin: A new alternative natural crosslinking agent for fixing heterograft tissue10.5090/kjtcs.2011.44.3.197 ↗
21. Genipin cross-linked alginate-chitosan microcapsules: Membrane characterization and optimization of cross-linking reaction10.1021/bm050862y ↗
22. Strong and bioactive gelatin–graphene oxide nanocomposites10.1039/c1sm05321c ↗
23. Preparation and characterization of genipin cross-linked porous chitosan–collagen–gelatin scaffolds using chitosan–CO2 solution10.1016/j.carbpol.2013.10.060 ↗
24. Poroviscoelastic characterization of particle-reinforced gelatin gels using indentation and homogenization10.1016/j.jmbbm.2011.01.009 ↗
25. Comparative study of hydroxyapatite/gelatin composites reinforced with bio-inert ceramic particles10.1016/j.ceramint.2011.12.073 ↗
26. Nature of graphene edges: A review10.1143/JJAP.50.070101 ↗
27. Graphene and graphene oxide: Synthesis, properties, and applications10.1002/adma.201001068 ↗
28. Lee, J.J., Shin, Y.C., Song, S.-J., Cha, J.M., Hong, S.W., Lim, Y.-J., Jeong, S.J., Han, D.-W., and Kim, B. (2018). Dicalcium phosphate coated with graphene synergistically increases osteogenic differentiation in vitro. Coatings, 8.10.3390/coatings8010013 ↗
29. Fabrication of highly porous biodegradable monoliths strengthened by graphene oxide and their adsorption of metal ions10.1016/j.carbon.2010.10.024 ↗
30. Chitosan-graphene oxide 3D scaffolds as promising tools for bone regeneration in critical-size mouse calvarial defects10.1038/s41598-017-16599-5 ↗
31. Novel gelatin–PHEMA porous scaffolds for tissue engineering applications10.1039/c2sm25536g ↗
32. Fabrication of transparent and ultraviolet shielding composite films based on graphene oxide and cellulose acetate10.1016/j.carbpol.2015.01.034 ↗
33. Processing and characterization of electrospun graphene oxide/polyurethane composite nanofibers for stent coating10.1016/j.cej.2015.01.105 ↗
34. Synthesis and characterization of graphene oxide/carboxymethylcellulose/alginate composite blend films10.1016/j.carbpol.2014.03.037 ↗
35. Mechanism and kinetics of the crosslinking reaction between biopolymers containing primary amine groups and genipin10.1002/pola.10960 ↗
36. Genipin-crosslinked chitosan/gelatin blends for biomedical applications10.1007/s10856-007-3212-5 ↗
37. Molecular structural properties of extracted gelatin from Yak skin as analysed based on molecular weight10.1080/10942912.2017.1300813 ↗
38. Synthesis and characterization of gelatin-chitosan nanocomposite to explore the possible use as drug delivery vehicle
39. Characterization of a graphene oxide membrane fuel cell10.1016/j.jpowsour.2014.08.071 ↗
40. Graphene production via electrochemical reduction of graphene oxide: Synthesis and characterisation10.1016/j.cej.2014.04.004 ↗
41. Effect of sodium acetate and drying temperature on physicochemical and thermomechanical properties of gelatin films10.1016/j.foodhyd.2014.10.009 ↗
42. Impact of electron beam irradiation on fish gelatin film properties10.1016/j.foodchem.2015.03.034 ↗
43. Mechanical and antibacterial properties of novel high performance chitosan/nanocomposite films10.1016/j.ijbiomac.2015.02.016 ↗
44. Synthesis and characterization of chitosan derivatives with dual-antibacterial functional groups10.1016/j.ijbiomac.2015.01.056 ↗
45. Optical and thermo-mechanical properties of composite films based on fish gelatin/rice flour fabricated by casting technique10.1016/j.porgcoat.2015.02.016 ↗
46. Fabrication and characterization of cross-linked gelatin electro-spun nano-fibers10.4236/jbise.2010.312145 ↗
47. Gong, Y., Yu, Y., Kang, H., Chen, X., Liu, H., Zhang, Y., Sun, Y., and Song, H. (2019). Synthesis and Characterization of Graphene Oxide/Chitosan Composite Aerogels with High Mechanical Performance. Polymers (Basel), 11.10.3390/polym11050777 ↗
48. Thermal performance and shape-stabilization of comb-like polymeric phase change materials enhanced by octadecylamine-functionalized graphene oxide10.1016/j.enconman.2018.05.014 ↗
49. Improving the thermal and mechanical properties of polysulfone by incorporation of graphene oxide10.1016/j.compositesb.2013.11.018 ↗
50. Fabrication and control of CT number through polymeric composites based on coronary plaque CT phantom applications10.1117/1.JMI.3.1.016001 ↗