journal article·2019-09-16Open access

Novel Edible Coating with Antioxidant and Antimicrobial Activities Based on Whey Protein Isolate Nanofibrils and Carvacrol and Its Application on Fresh-Cut Cheese

Qiannan Wang, Hongliang Yu, Bo Tian, Bin Jiang, Jing Xu, Dongmei Li, Zhibiao Feng, Chunhong Liu

Coatings· Volume 9· Issue 9

Abstract

The composition and properties of edible coatings (ECs) will significantly influence their effects of food preservation. For the first time, whey protein isolates nanofibers (WPNFs), as a novel material with high hydrophobicity and antioxidant activity, combined with carvacrol (CA) as an antimicrobial agent and glycerol (Gly) as a plasticizer, was used to prepare edible coating (WPNFs-CA/Gly) for preserving fresh-cut Cheddar cheese. The prepared WPNFs and ECs emulsions have been investigated with transmission electron microscopy. Furthermore, the antioxidant activity of ECs emulsions, antimicrobial activity of edible films, and the physical properties of edible films, such as micromorphology, thickness, transparency, and moisture content, have also been evaluated. The weight losses and physical characteristics of both coated and uncoated fresh-cut Cheddar cheese samples have been assessed during storage. The DPPH free radical scavenging rate of WPNFs-CA/Gly emulsion was up to 67.89% and the reducing power was 0.821, which was higher than that of WPI-CA/Gly emulsions. The antimicrobial activity of WPNFs-CA/Gly films was nearly 2.0-fold higher than that of WPNFs/Gly films for the presence of CA. The WPNFs-CA/Gly films had smooth and continuous surfaces, and the transparency reached 49.7% and the moisture content was 26.0%, which was better than that of WPI-CA/Gly films. Furthermore, Cheddar cheese with WPNFs-CA/Gly coatings has shown lower weight losses (15.23%) and better textural properties than those uncoated samples. This in-depth study has provided a valuable and noteworthy approach about the novel edible coating material.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Nanocomposite Films for Food Packaging · Microplastics and Plastic Pollution · Proteins in Food Systems

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Applied Chemistry, College of Science, Northeast Agricultural University, Harbin 150030, China

College of Food Science, Northeast Agricultural University, Harbin 150030, China

Funding

National Key Research and Development Program of China · Heilongjiang Nature Scientific Foundation Project

References (44)

Show source-provided references
1. Peptides are building blocks of heat-induced fibrillar protein aggregates of β-lactoglobulin formed at pH 210.1021/bm7014224 ↗
2. Liu, C., Liu, W., Feng, Z., and Li, D. (2014). Aggregation of Whey Protein Hydrolysate Using Alcalase 2.4 L. PLoS ONE, 9.10.1371/journal.pone.0109439 ↗
3. Irreversible self-assembly of ovalbumin into fibrils and the resulting network rheology10.1016/S0141-8130(03)00076-X ↗
4. Micrometer-Sized Fibrillar Protein Aggregates from Soy Glycinin and Soy Protein Isolate10.1021/jf0718897 ↗
5. Fibril formation from pea protein and subsequent gel formation10.1021/jf4055215 ↗
6. Jiang, B., Na, J., Wang, L., Li, D., Liu, C., and Feng, Z. (2019). Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation. Foods, 8.10.3390/foods8010034 ↗
7. Impact of stirring speed on beta-lactoglobulin fibril formation10.1007/s10068-016-0093-8 ↗
8. Assessing the potential of whey protein fibril as emulsifier10.1016/j.jfoodeng.2017.12.006 ↗
9. Formation mechanisms, handling and digestibility of food protein nanofibrils10.1016/j.tifs.2015.05.005 ↗
10. Fibrillar structures in food10.1039/C1FO10163C ↗
11. Inhibiting, promoting, and preserving stability of functional protein fibrils10.1039/C1SM06643A ↗
12. Globular plant protein aggregates for stabilization of food foams and emulsions10.1016/j.tifs.2017.07.013 ↗
13. Effect of chitosan-carvacrol edible coatings on the quality and shelf life of tilapia (Oreochromis niloticus) fillets stored in ice10.1590/1678-457X.6841 ↗
14. Structural design principles for delivery of bioactive components in nutraceuticals and functional foods10.1080/10408390902841529 ↗
15. Comprehensive review on application of edible film on meat and meat products: An eco-friendly approach10.1080/10408398.2014.986563 ↗
16. Natural Antimicrobial/Antioxidant Agents in Meat and Poultry Products as Well as Fruits and Vegetables: A Review10.1080/10408398.2016.1194256 ↗
17. Feng, Z., Li, L., Wang, Q., Wu, G., Liu, C., Jiang, B., and Xu, J. (2019). Effect of Antioxidant and Antimicrobial Coating based on Whey Protein Nanofibrils with TiO(2) Nanotubes on the Quality and Shelf Life of Chilled Meat. Int. J. Mol. Sci., 20.10.3390/ijms20051184 ↗
18. Effect of sodium alginate-based edible coating containing different anti-oxidants on quality and shelf life of refrigerated bream (Megalobrama amblycephala)10.1016/j.foodcont.2010.10.012 ↗
19. Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout10.1016/j.foodchem.2009.10.006 ↗
20. Effect of chitosan-carvacrol coating on the quality of Pacific white shrimp during iced storage as affected by caprylic acid10.1016/j.ijbiomac.2017.07.180 ↗
21. Application of edible coating with starch and carvacrol in minimally processed pumpkin10.1007/s13197-016-2171-6 ↗
22. Investigation of different coating application methods on the performance of edible coatings on Mozzarella cheese10.1016/j.lwt.2013.11.006 ↗
23. Heat-induced whey protein isolate fibrils: Conversion, hydrolysis, and disulphide bond formation10.1016/j.idairyj.2006.10.002 ↗
24. Formation of Whey Protein Isolate Nanofibrils by Endoproteinase GluC and Their Emulsifying Properties10.1016/j.foodhyd.2019.03.004 ↗
25. Screening of radical scavenging activity of some medicinal and aromatic plant extracts10.1016/j.foodchem.2003.05.007 ↗
26. Multiscale characterization of individualized β-lactoglobulin microgels formed upon heat treatment under narrow pH range conditions10.1021/la900501n ↗
27. Characterization of chitosan edible films obtained with various polymer concentrations and drying temperatures10.1016/j.ijbiomac.2018.03.057 ↗
28. The preparation, characterization and in vitro application evaluation of soluble soybean polysaccharide films incorporated with cinnamon essential oil nanoemulsions10.1016/j.ijbiomac.2018.01.145 ↗
29. Oregano essential oil loaded soybean polysaccharide films: Effect of Pickering type immobilization on physical and antimicrobial properties10.1016/j.foodhyd.2018.08.011 ↗
30. Multiple steps during the formation of beta-lactoglobulin fibrils10.1021/bm034096b ↗
31. Ethanol-induced whey protein gels as carriers for lutein droplets10.1016/j.foodhyd.2016.05.013 ↗
32. Characterisation and use of β-lactoglobulin fibrils for microencapsulation of lipophilic ingredients and oxidative stability thereof10.1016/j.jfoodeng.2014.06.026 ↗
33. Jiang, B., Na, J., Wang, L., Li, D., Liu, C., and Feng, Z. (2019). Eco-Innovation in Reusing Food By-Products: Separation of Ovalbumin from Salted Egg White Using Aqueous Two-Phase System of PEG 1000/(NH4)2SO4. Polymers, 11.10.3390/polym11020238 ↗
34. Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures10.1111/j.1745-7270.2007.00320.x ↗
35. Binding of curcumin to beta-lactoglobulin and its effect on antioxidant characteristics of curcumin10.1016/j.foodchem.2013.02.099 ↗
36. Zein-caseinate composite nanoparticles for bioactive delivery using curcumin as a probe compound10.1016/j.foodhyd.2018.04.037 ↗
37. Enhancing the aqueous solubility of curcumin at acidic condition through the complexation with whey protein nanofibrils10.1016/j.foodhyd.2018.09.001 ↗
38. Cold-set hydrogels made of whey protein nanofibrils with different divalent cations10.1016/j.ijbiomac.2016.05.009 ↗
39. Improving the shelf life of low-fat cut cheese using nanoemulsion-based edible coatings containing oregano essential oil and mandarin fiber10.1016/j.foodcont.2017.01.001 ↗
40. Functional Materials in Food Nanotechnology10.1111/j.1750-3841.2006.00195.x ↗
41. Characterization of fish protein films incorporated with essential oils of clove, garlic and origanum: Physical, antioxidant and antibacterial properties10.1016/j.lwt.2014.04.024 ↗
42. Chitosan films incorporated with Apricot (Prunus armeniaca) kernel essential oil as active food packaging material10.1016/j.foodhyd.2018.07.003 ↗
43. Edible coating based on whey protein isolate nanofibrils for antioxidation and inhibition of product browning10.1016/j.foodhyd.2017.12.028 ↗
44. Processing of soft Hispanic cheese (“queso fresco”) using thermo-sonicated milk: A study of physicochemical characteristics and storage life