Preparation and Characterization of Bioactive Chitosan-Based Films Incorporated with Olive Leaves Extract for Food Packaging Applications
Source abstract
Abstract
Chitosan films with olive leaf extract (OLE) incorporated at different concentrations were characterized regarding their antimicrobial, antioxidant and some relevant physical properties (i.e., solubility, water vapor permeability, and tensile properties). Results indicate that the active films have substantial antimicrobial activity against Listeria monocytogenes and Campylobacter jejuni mostly extending the microorganisms lag phase. A lower level of inhibition was found in the case of Escherichia coli. However, the OLE seems not to improve the intrinsic antimicrobial properties of the chitosan itself, except for C. jejuni. These results were confirmed with in situ testing using chicken. The chitosan films with OLE exhibited antioxidant activity, increasing with the OLE concentration, from 0.04 to 0.15 g/L ascorbic acid equivalents, corresponding to films with 10%–30% OLE relative to the chitosan. Chitosan films loaded with OLE exhibited a higher solubility in food simulants and a reduced permeability against water vapor. Overall, the combination of OLE and chitosan allows to obtain a promising active bio-based packaging solution for addressing safety and quality issues.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Nanocomposite Films for Food Packaging · Essential Oils and Antimicrobial Activity · Postharvest Quality and Shelf Life Management
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Universidade Católica Portuguesa, CINATE, Escola Superior de Biotecnologia, 4169-005 Porto, Portugal
Food Packaging Lab., DeFENS, Department of Food, Environmental and Nutritional Sciences, University of Milan, via Celoria 2–I, 20133 Milan, Italy
Universidade Católica Portuguesa, CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
Funding
PDR 2020, Portugal 2020 and the EU GRUPO OPERACIONAL ID 228, CAMPYFREE: Estratégias de controlo de Campylobacter em carne de aves e produtos cárneos derivados
References (50)
Show source-provided references
1. Environmental impacts of food waste in europe10.1016/j.wasman.2018.04.038 ↗
2. Sustainable alternative for food packaging: Chitosan biopolymer—A review
3. Campylobacter spp. as a foodborne pathogen: A review10.3389/fmicb.2011.00200 ↗
4. Christine, E.R.D., Tim, A., Richard, A.S., Dean, O.C., and Hans, P.R. (2017). Epidemiology, Cost, and Risk Analysis of Foodborne Disease (Chapter 2) in Foodborne Diseases, Academic Press. [3rd ed.].
5. Volpe, S., Cavella, S., and Torrieri, E. (2019). Biopolymer coatings as alternative to modified atmosphere packaging for shelf life extension of minimally processed apples. Coatings, 9.10.3390/coatings9090569 ↗
6. Development and characterization of biodegradable chitosan films containing two essential oils10.1016/j.ijbiomac.2014.11.046 ↗
7. European Commission (2011). Commission Regulation (EU), No. 10/2011 of 14 January 2011. Off. J. Eur. Union, 54, 1–89.
8. Preparation and characterization of biocomposite film based on chitosan and kombucha tea as active food packaging10.1016/j.ijbiomac.2017.12.028 ↗
9. Chitosan dipping or oregano oil treatments, singly or combined on modified atmosphere packaged chicken breast meat10.1016/j.ijfoodmicro.2012.04.002 ↗
10. Lozano-Navarro, J.I., Díaz-Zavala, N.P., Velasco-Santos, C., Melo-Banda, J.A., Páramo-García, U., Paraguay-Delgado, F., García-Alamilla, R., Martínez-Hernández, A.L., and Zapién-Castillo, S. (2018). Chitosan-starch films with natural extracts: Physical, chemical, morphological and thermal properties. Materials, 11.10.3390/ma11010120 ↗
11. Treviño-Garza, M.Z., Correa-Cerón, R.C., Ortiz-Lechuga, E.G., Solís-Arévalo, K.K., Castillo-Hernández, S.L., Gallardo-Rivera, C.T., and Arévalo Niño, K. (2019). Effect of linseed (Linum usitatissimum) mucilage and chitosan edible coatings on quality and shelf-life of fresh-cut cantaloupe (Cucumis melo). Coatings, 9.10.3390/coatings9060368 ↗
12. Evaluation of different factors affecting antimicrobial properties of chitosan10.1016/j.ijbiomac.2016.01.022 ↗
13. Chitosan based nanocomposite films and coatings: Emerging antimicrobial food packaging alternatives10.1016/j.tifs.2020.01.002 ↗
14. Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: A review10.1016/j.ijbiomac.2020.08.153 ↗
15. Bioactive packaging materials from edible chitosan polymer—Antimicrobial activity assessment on dairy-related contaminants10.1111/j.1365-2621.2003.tb05806.x ↗
16. Chitosan films and coatings containing essential oils: The antioxidant and antimicrobial activity, and application in food systems10.1016/j.foodres.2016.10.004 ↗
17. Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract10.1016/j.foodhyd.2010.04.003 ↗
18. A chitosan-based coating with or without clove oil extends the shelf life of cooked pork sausages in refrigerated storage10.1016/j.meatsci.2015.10.003 ↗
19. Souza, V.G.L., Pires, J.R.A., Rodrigues, C., Freitas Rodrigues, P., Lopes, A., Silva, R.J., Caldeira, J., Duarte, M.P., Braz Fernandes, F., and Coelhoso, I.M. (2019). Physical and morphological characterization of chitosan/montmorillonite films incorporated with ginger essential oil. Coatings, 9.10.3390/coatings9110700 ↗
20. Zhou, Y., Xu, T., Zhang, Y., Zhang, C., Lu, Z., Lu, F., and Zhao, H. (2019). Effect of tea polyphenols on curdlan/chitosan blending film properties and its application to chilled meat preservation. Coatings, 9.10.3390/coatings9040262 ↗
21. Li, N., Liu, W., Shei, Y., Mei, J., and Xie, J. (2019). Coating effects of ε-polylysine and rosmarinic acid combined with chitosan on the storage quality of fresh half-smooth tongue sole (Cynoglossus semilaevis Günther) fillets. Coatings, 9.10.3390/coatings9040273 ↗
22. Effect of chitosan coating combined with pomegranate peel extract on the quality of Pacific white shrimp during iced storage10.1016/j.foodcont.2015.07.011 ↗
23. Li, C., Pei, J., Zhu, S., Song, Y., Xiong, X., and Xue, F. (2020). Development of chitosan/peptide films: Physical, antibacterial and antioxidant properties. Coatings, 10.10.3390/coatings10121193 ↗
24. Composition and antioxidant activity of olive leaf extracts from Greek Olive cultivars10.1007/s11746-009-1517-x ↗
25. Effect of oleuropein on the oxidative stability and storage quality of Tabaq-Maz, fried mutton ribs10.1016/j.fbio.2015.08.002 ↗
26. Antibacterial effect of olive (Olea europaea L.) leaves extract in raw peeled undeveined shrimp (Penaeus semisulcatus)10.1016/j.ijvsm.2014.04.002 ↗
27. Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork10.1002/pts.717 ↗
28. Combined effects of two kinds of essential oils on physical, mechanical and structural properties of chitosan films10.1016/j.foodhyd.2013.10.013 ↗
29. Determination of oleuropein in herbal preparation and Olea europaea L. extracts by HPLC
30. Determination of degree of deacetylation of chitosan—Comparision of methods
31. Modified 2,2-azino-bis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2′-diphenyl-1- picrylhydrazyl (DPPH) methods10.1021/jf051960d ↗
32. Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities10.1016/j.ijfoodmicro.2014.04.029 ↗
33. Chitosan based biomaterials for tissue engineering10.1016/j.eurpolymj.2012.12.009 ↗
34. Polyphenols and secoiridoids in raw material (Olea europaea L. leaves) and commercial food supplements10.1007/s00217-016-2756-3 ↗
35. Assessment of the antimicrobial activity of olive leaf extract against foodborne bacterial pathogens
36. In vitro antibacterial activity of olive leaves10.1046/j.1439-0507.2003.00859.x ↗
37. Phenolic compounds and antibacterial activity of olive (Olea europaea L. Cv. Cobrancosa) leaves10.3390/12051153 ↗
38. Antibacterial activity of olive (Olea europaea) leaves and arugula (Eruca sativa) seeds extract
39. Antimicrobial activity of olive leaf aqueous extract
40. Characterization of antimicrobial polylactic acid based films10.1016/j.jfoodeng.2013.05.043 ↗
41. Diffusion of acetic and propionic acids from chitosan-based antimicrobial packaging films10.1111/j.1365-2621.2000.tb13584.x ↗
42. Antimicrobial activity of commercial Olea europaea (olive) leaf extract10.1016/j.ijantimicag.2008.10.026 ↗
43. Active food packaging prepared with chitosan and olive pomace10.1016/j.foodhyd.2017.08.007 ↗
44. Phenolic content and antioxidant activity of olive by-products and antioxidant film containing olive leaf extract10.1016/j.foodchem.2016.06.001 ↗
45. Antioxidant activity of phenolics extracted from Olea europaea L. leaves10.1016/S0308-8146(99)00221-6 ↗
46. Ngo, D.-H., and Kim, S.-K. (2014). Antioxidant effects of chitin, chitosan, and their derivatives. Mar. Carbohydr. Fundam. Appl. Part B, 15–31.10.1016/B978-0-12-800268-1.00002-0 ↗
47. Antioxidant activity of water-soluble chitosan derivatives10.1016/S0960-894X(01)00285-2 ↗
48. Antioxidant activities of chitosans and its derivatives in in vitro and in vivo studies10.1016/j.carbpol.2018.07.016 ↗
49. Kurek, M., Galus, S., and Debeaufort, F. (2014). Surface, mechanical and barrier properties of bio-based composite films based on chitosan and whey protein. Food Packag. Shelf Life, 56–67.10.1016/j.fpsl.2014.01.001 ↗
50. Razavi, M.S., Golmohammadi, A., Nematollahzadeh, A., Rovera, C., and Farris, S. (2021). Fish gelatin-bacterial cellulose nanocrystals active films containing cinnamon essential oil nanocapsules. Food Biophys.