Comparison of Antimicrobial Activity of Chitosan Nanoparticles against Bacteria and Fungi
Source abstract
Abstract
Chitosan nanoparticles (CSNPs) have attracted wide interest; however, there has been no substantial information about a direct comparison of the antimicrobial activity of CSNPs on bacteria and fungi. Thus, in this study, simple, economically feasible CSNPs were synthesized and assessed for their antimicrobial activity. This investigation indicated that the coordination inducing effect of CSNPs could dissociate the tryptophan (Trp) and tyrosine (Tyr) residue groups on the peptide chain of the bovine serum albumin (BSA) molecule, thereby increasing the absorption intensity. The growth of E. coli and S. aureus could be completely inhibited when the concentration of CSNPs in the solution was higher than 0.6 mg/mL. The CSNPs showed more potent antibacterial activity against Gram-negative bacteria (E. coli) than against Gram-positive bacteria (S. aureus). In addition, the CSNPs were effective at initiating cellular leakage of fungal mycelia and damping off fungal pathogens, and their antifungal effects were stronger on P. steckii than on A. oryzae. Furthermore, the antimicrobial activity of the CSNPs was found to be more effective against bacteria than against fungi. This study thus ascertained the antimicrobial activity of synthesized CSNPs against different microorganisms, as well as their different degrees of inhibition.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Nanocomposite Films for Food Packaging · Nanoparticles: synthesis and applications · Advanced Drug Delivery Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Key Laboratory of Grain and Oil Processing and Food Safety of Sichuan Province, College of Food and Bioengineering, Xihua University, Chengdu 610039, China
Key Laboratory of Food Non-Thermal Technology, Engineering Technology Research Center of Food Non-Thermal, Yibin Xihua University Research Institute, Yibin 644004, China
Department of Food Science and Engineering, College of Landscape Architecture, Shangqiu University, Shangqiu 476000, China
Key Laboratory of Storage of Agricultural Products, Ministry of Agriculture and Rural Affairs, Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering Technology Research Center for Preservation of Agricultural Products, Tianjin 300384, China
Funding
Key Laboratory of Storage of Agricultural Products, Ministry of Agriculture and Rural Affairs · the Science and technology support program of Sichuan · Science and technology support program of Yibin · Chengdu Science and Technology Project- key research and development program · Xihua University Graduate Innovation Fund Project · College Students innovation and entrepreneurship training program of Xihua University · Innovation Team Construction Program of Sichuan Education Department
References (66)
Show source-provided references
1. Chitosan based nanocomposite films and coatings: Emerging antimicrobial food packaging alternatives10.1016/j.tifs.2020.01.002 ↗
2. Improving the integrity of natural biopolymer films used in food packaging by crosslinking approach: A review10.1016/j.ijbiomac.2017.06.093 ↗
3. Optimisation, antioxidant attributes, stability and release behaviour of carboxymethyl cellulose films incorporated with nanoencapsulated vitamin E10.1016/j.porgcoat.2019.05.026 ↗
4. Dedloff, M.R., Effler, C.S., Holban, A.M., and Gestal, M.C. (2019). Use of biopolymers in mucosally-administered vaccinations for respiratory disease. Materials, 12.10.3390/ma12152445 ↗
5. Active packaging from chitosan-titanium dioxide nanocomposite film for prolonging storage life of tomato fruit10.1016/j.ijbiomac.2018.01.124 ↗
6. Plasma treated polyethylene terephthalate/polypropylene films assembled with chitosan and various preservatives for antimicrobial food packaging10.1016/j.colsurfb.2013.09.052 ↗
7. Perspectives for chitosan based antimicrobial films in food applications10.1016/j.foodchem.2008.11.047 ↗
8. Preparation of chitosan nanoparticles by TPP ionic gelation combined with spray drying, and the antibacterial activity of chitosan nanoparticles and a chitosan nanoparticle–amoxicillin complex10.1007/s11164-016-2428-8 ↗
9. Synthesis, characterization, and antibacterial activity of chitosan/TiO2 nanocomposite against Xanthomonas oryzae pv10.1016/j.carbpol.2016.07.070 ↗
10. Layer-by-layer immobilization of lysozyme–chitosan–organic rectorite composites on electrospun nanofibrous mats for pork preservation10.1016/j.foodres.2012.06.026 ↗
11. Chitosan as antimicrobial agent: Applications and mode of action10.1021/bm034130m ↗
12. Chitosan-based nanomaterials: A state-of-the-art review10.1016/j.ijbiomac.2013.04.043 ↗
13. Antifungal activity of chitosan nanoparticles and correlation with their physical properties10.1155/2012/632698 ↗
14. Encapsulation of curcumin in alginate-chitosan-pluronic composite nanoparticles for delivery to cancer cells10.1016/j.nano.2009.05.009 ↗
15. Plasmas ozone inactivation of Legionella in deionized water and wastewater10.1007/s11356-018-1233-x ↗
16. Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets10.1016/j.ijfoodmicro.2009.04.013 ↗
17. Preparation and antibacterial activity of chitosan nanoparticles10.1016/j.carres.2004.09.007 ↗
18. Chitosan nanoparticles preparation and applications10.1007/s10311-017-0670-y ↗
19. Evaluation of different factors affecting antimicrobial properties of chitosan10.1016/j.ijbiomac.2016.01.022 ↗
20. Chitosan derivatives with antimicrobial, antitumour and antioxidant activities—A review10.2174/138161211798194468 ↗
21. Synthesis and optimization of chitosan nanoparticles: Potential applications in nanomedicine and biomedical engineering
22. Chitosan nanoparticles as delivery systems for doxorubicin10.1016/S0168-3659(01)00294-2 ↗
23. Characterization of tara gum edible films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study10.1016/j.foodhyd.2014.09.023 ↗
24. Comparing the effectiveness of chitosan and nanochitosan coatings on the quality of refrigerated silver carp fillets10.1016/j.foodcont.2014.11.015 ↗
25. Multifunctional betanin nanoliposomes-incorporated gelatin/chitosan nanofiber/ZnO nanoparticles nanocomposite film for fresh beef preservation10.1016/j.meatsci.2020.108161 ↗
26. Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique10.1016/j.colsurfb.2011.09.042 ↗
27. Supermolecular film crosslinked by polyoxometalate and chitosan with superior antimicrobial effect10.1016/j.ijbiomac.2020.03.139 ↗
28. Insight into the interaction between chitosan and bovine serum albumin10.1016/j.carbpol.2017.08.068 ↗
29. Multivalent and synergistic chitosan oligosaccharide-Ag nanocomposites for therapy of bacterial infection10.1038/s41598-020-67139-7 ↗
30. Chitosan-hydroxycinnamic acid conjugates: Preparation, antioxidant and antimicrobial activity10.1016/j.foodchem.2013.10.019 ↗
31. Biocontrol potential of Streptomyces hydrogenans strain DH16 toward Alternaria brassicicola to control damping off and black leaf spot of Raphanus sativus10.3389/fpls.2016.01869 ↗
32. Synthesis and application of chitosan-copper nanoparticles on damping off causing plant pathogenic fungi10.1016/j.ijbiomac.2019.11.179 ↗
33. Coatings with chitosan and phenolic-rich extract from acerola (Malpighia emarginata D.C.) or jabuticaba (Plinia jaboticaba (Vell.) Berg) processing by-product to control rot caused by Lasiodiplodia spp. in papaya (Carica papaya L.) fruit10.1016/j.ijfoodmicro.2020.108694 ↗
34. Saponin-loaded chitosan nanoparticles and their cytotoxicity to cancer cell lines in vitro10.1016/j.carbpol.2010.11.056 ↗
35. Interests of chitosan nanoparticles ionically cross-linked with tripolyphosphate for biomedical applications10.1016/j.progpolymsci.2016.06.002 ↗
36. Effect of cryoprotection on particle size stability and preservation of chitosan nanoparticles with and without hyaluronate or alginate coating10.1016/j.jsps.2016.12.008 ↗
37. Effects of acetonitrile on adsorption behavior of bovine serum albumin onto synthetic calcium hydroxyapatite particles10.1006/jcis.2002.8495 ↗
38. Synthesis, antibacterial activity and effect of O-carboxymethyl chitosan on cell membrane protein
39. Turbidity and rheological properties of bovine serum albumin/pectin coacervates: Effect of salt concentration and initial protein/polysaccharide ratio10.1016/j.carbpol.2012.01.019 ↗
40. A novel biomimetic chitosan-based nanocarrier with suppression of the protein-nanocarrier interactions10.1016/j.matlet.2012.02.126 ↗
41. Interaction between low molecular weight chitosan derivatives and bovine serum album
42. Spray-dried rice starch: Comparative evaluation of direct compression fillers10.3109/03639049609063212 ↗
43. Drioli, E., and Giorno, L. (2016). Encapsulation Efficiency. Encyclopedia of Membranes, Springer.10.1007/978-3-642-40872-4 ↗
44. Preparation and characterization of water-soluble chitosan nanoparticles as protein delivery system10.1155/2010/898910 ↗
45. Yan, Q., Weng, J., Wu, X., Wang, W., Yang, Q., Guo, F., Wu, D., Song, Y., Chen, F., and Yang, G. (2020). Characteristics, cryoprotection evaluation and in vitro release of BSA-loaded chitosan nanoparticles. Mar. Drugs, 18.10.3390/md18060315 ↗
46. Chitosan nanoparticles: Polyphosphates cross-linking and protein delivery properties10.1016/j.ijbiomac.2019.06.062 ↗
47. Tao, W.S., Li, W., and Jiang, Y.M. (1995). Protein Molecular Foundation, Higher Education Press.
48. Essential oils: Their antibacterial properties and potential applications in foods—A review10.1016/j.ijfoodmicro.2004.03.022 ↗
49. Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes10.1016/j.colsurfb.2019.01.045 ↗
50. Antimicrobial properties of starch films incorporated with chitosan nanoparticles: In vitro and in vivo evaluation10.1016/j.carbpol.2019.115602 ↗
51. Quercetin-grafted chitosan prepared by free radical grafting: Characterization and evaluation of antioxidant and antibacterial properties10.1007/s13197-020-04263-2 ↗
52. Antibacterial activity of high-molecular-weight and low-molecular-weight chitosan upon oral pathogens10.4103/JCD.JCD_300_18 ↗
53. Chitosan nanoparticles enhance the antibacterial activity of the native polymer against bovine mastitis pathogens10.1016/j.carbpol.2019.02.016 ↗
54. Abdallah, Y., Liu, M., Ogunyemi, S.O., Ahmed, T., Fouad, H., Abdelazez, A., Yan, C., Yang, Y., Chen, J., and Li, B. (2020). Bioinspired green synthesis of chitosan and zinc oxide nanoparticles with strong antibacterial activity against rice pathogen Xanthomonas oryzae pv. oryzae. Molecules, 25.10.3390/molecules25204795 ↗
55. Exploring the physicochemical and antimicrobial properties of gold-chitosan hybrid nanoparticles composed of varying chitosan amounts10.1016/j.ijbiomac.2020.08.046 ↗
56. Atomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureus10.1016/j.ultramic.2008.04.015 ↗
57. Matica, M.A., Aachmann, F.L., Tondervik, A., Sletta, H., and Ostafe, V. (2019). Chitosan as a wound dressing starting material: Antimicrobial properties and mode of action. Int. J. Mol. Sci., 20.10.3390/ijms20235889 ↗
58. Chitosan derivatives and their based nanoparticles: Ultrasonic approach to the synthesis, antimicrobial and transfection properties10.1016/j.carbpol.2020.116478 ↗
59. Comparative antibacterial and antifungal activities of sulfur nanoparticles capped with chitosan10.1016/j.micpath.2020.104178 ↗
60. Recent developments in antibacterial and antifungal chitosan and its derivatives10.1016/j.carbpol.2017.02.001 ↗
61. An inhibitory action of chitosan nanoparticles against pathogenic bacteria and fungi and their potential applications as biocompatible antioxidants10.1016/j.micpath.2017.11.043 ↗
62. Membrane fluidity determines sensitivity of filamentous fungi to chitosan10.1111/j.1365-2958.2009.07039.x ↗
63. Effect of chitosan on hyphal growth and spore germination of plant pathogenic and biocontrol fungi
64. Chitosan permeabilizes the plasma membrane and kills cells of Neurospora crassa in an energy dependent manner10.1016/j.fgb.2009.02.010 ↗
65. A Review of the antimicrobial activity of chitosan10.1590/S0104-14282009000300013 ↗
66. Disruption of protein synthesis as antifungal mode of action by chitosan10.1016/j.ijfoodmicro.2013.03.025 ↗