journal article·2023-08-23Open access

Evaluation of Antimicrobial and Antioxidant Activities of Alginate Edible Coatings Incorporated with Plant Extracts

Ana Lopes, Adma Melo, Cristina Caleja, Eliana Pereira, Tiane Finimundy, Tiago Afonso, Sara Silva, Marija Ivanov, Marina Soković, Freni Tavaria, Lillian Barros, Manuela Pintado

Coatings· Volume 13· Issue 9

Abstract

Plant extracts (PEs) are natural substances that perform interesting bioactivities. However, they have some limitations. In this work, PEs from licorice, eucalyptus, sage, and thyme were produced and characterized. These extracts showed good bioactive properties. Alginate-based edible coatings and films incorporated with the extracts were produced and their antimicrobial and antioxidant properties were determined. The results showed that the coatings completely inhibited or reduced the growth of all bacteria (E. coli, P. aeruginosa, B. cereus, L. monocytogenes, and S. aureus), except the coating with sage extract. However, only the films incorporated with licorice and eucalyptus could inhibit the growth of Gram-positive bacteria and none of the films were able to inhibit Gram-negative bacteria. The film incorporated with sage extract was the one with the best antifungal property. All films possess a good antioxidant property (ABTS: 942.52–5654.62; DPPH: 1040.67–5162.93 Trolox equivalents (µM)/mg film). Based on the results, it is possible to conclude that alginate coatings and films with PEs present good bioactive properties and can potentially be used as new, biodegradable packages. However, further experiments need to be performed to assess their safety profile, and to prove that they can be a real alternative to traditional food packaging.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Essential Oils and Antimicrobial Activity · Nanocomposite Films for Food Packaging · Phytochemicals and Antioxidant Activities

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratório Associado, Escola Superior de Biotecnologia, CBQF—Centro de Biotecnologia e Química Fina, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal

Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal

Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal

Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia

Funding

BIOma—Soluções integradas de BIOeconomia para a Mobilização da cadeia Agroalimentar · Fundo Europeu de Desenvolvimento Regional (FEDER) · National Funds from FCT—Fundação para a Ciência e a Tecnologia

References (74)

Show source-provided references
1. Effect of Plasticizer Type and Essential Oils on Mechanical, Physicochemical, and Antimicrobial Characteristics of Gelatin, Starch, and Pectin-Based Films10.1111/jfpp.14480 ↗
2. Edible Cassava Starch Films Carrying Rosemary Antioxidant Extracts for Potential Use as Active Food Packaging10.1016/j.foodhyd.2016.09.034 ↗
3. Development and Characterization of Sodium Alginate Based Active Edible Films Incorporated with Essential Oils of Some Medicinal Plants10.1016/j.ijbiomac.2019.12.093 ↗
4. Development of Essential Oil Incorporated Active Film Based on Biodegradable Blends of Poly (Lactide)/Poly (Butylene Adipate-Co-Terephthalate) for Food Packaging Application10.1007/s41783-020-00099-5 ↗
5. Preparation, Characterization and Antimicrobial Activity of Polyvinyl Alcohol/Gum Arabic/Chitosan Composite Films Incorporated with Black Pepper Essential Oil and Ginger Essential Oil10.1016/j.ijbiomac.2020.02.176 ↗
6. Cross-Linked Methyl Cellulose Films with Murta Fruit Extract for Antioxidant and Antimicrobial Active Food Packaging10.1016/j.foodhyd.2016.03.020 ↗
7. Augusto, A., Dias, J.R., Campos, M.J., Alves, N.M., Pedrosa, R., and Silva, S.F.J. (2018). Influence of Codium tomentosum Extract in the Properties of Alginate and Chitosan Edible Films. Foods, 7.10.3390/foods7040053 ↗
8. Antioxidative and Antimicrobial Edible Chitosan Films Blended with Stem, Leaf and Seed Extracts of Pistacia terebinthus for Active Food Packaging10.1039/C7RA12070B ↗
9. Edible Polymers: Challenges and Opportunities
10. Biobased Materials for Active Food Packaging: A Review10.1016/j.foodhyd.2021.107419 ↗
11. Co-Concentration Effect of Silane with Natural Extract on Biodegradable Polymeric Films for Food Packaging10.1016/j.ijbiomac.2017.08.025 ↗
12. Fabrication and Comparison of Active Films from Chitosan Incorporating Different Spice Extracts for Shelf Life Extension of Refrigerated Pork10.1016/j.lwt.2020.110181 ↗
13. Kaur, N., Bains, A., Kaushik, R., Dhull, S.B., Melinda, F., and Chawla, P. (2021). A Review on Antifungal Efficiency of Plant Extracts Entrenched Polysaccharide-Based Nanohydrogels. Nutrients, 13.10.3390/nu13062055 ↗
14. Antimicrobial Hydrogels: Promising Materials for Medical Application10.2147/IJN.S154748 ↗
15. Advancements and Future Directions in the Antibacterial Wound Dressings—A Review
16. Physical, Mechanical and Barrier Properties of Corn Starch Films Incorporated with Plant Essential Oils10.1016/j.carbpol.2013.07.026 ↗
17. Physical, Mechanical and Antibacterial Properties of Alginate Film: Effect of the Crosslinking Degree and Oregano Essential Oil Concentration10.1016/j.jfoodeng.2011.05.023 ↗
18. Development of a Natural Preservative Obtained from Male Chestnut Flowers: Optimization of a Heat-Assisted Extraction Technique10.1039/C8FO02234H ↗
19. Bioactivity and Chemical Characterization in Hydrophilic and Lipophilic Compounds of Chenopodium ambrosioides L.10.1016/j.jff.2013.07.019 ↗
20. Phenolic Profiles of Cultivated, in Vitro Cultured and Commercial Samples of Melissa officinalis L. Infusions10.1016/j.foodchem.2012.07.107 ↗
21. Cellular Antioxidant Activity (CAA) Assay for Assessing Antioxidants, Foods, and Dietary Supplements10.1021/jf0715166 ↗
22. Melissa officinalis L. Decoctions as Functional Beverages: A Bioactive Approach and Chemical Characterization10.1039/C5FO00309A ↗
23. Antimicrobial Activity of Pomegranate Peel Extracts Performed by High Pressure and Enzymatic Assisted Extraction10.1016/j.foodres.2018.08.044 ↗
24. Characterization and Antimicrobial Properties of Food Packaging Methylcellulose Films Containing Stem Extract of Ginja Cherry10.1002/jsfa.6530 ↗
25. Active Whey Protein Edible Films and Coatings Incorporating Lactobacillus buchneri for Penicillium nordicum Control in Cheese10.1007/s11947-020-02465-2 ↗
26. Hierarchical Scheme for LC-MSn Identification of Chlorogenic Acids10.1021/jf026187q ↗
27. Basil as Functional and Preserving Ingredient in “Serra Da Estrela” Cheese10.1016/j.foodchem.2016.03.085 ↗
28. Bioactive Monoterpene Glycosides Conjugated with Gallic Acid from the Leaves of Eucalyptus globulus10.1016/j.phytochem.2007.08.030 ↗
29. Chemical Studies of Chinese Licorice-Roots. I. Elucidation of Five New Flavonoid Constituents from the Roots of Glycyrrhiza glabra L. Collected in Xinjiang10.1248/cpb.42.1056 ↗
30. Characterization of Phenolic Compounds and Antioxidant Properties of Glycyrrhiza glabra L. Rhizomes and Roots10.1039/C5RA03963K ↗
31. Saponins from European Licorice Roots (Glycyrrhiza glabra)10.1021/acs.jnatprod.8b00022 ↗
32. Antioxidant Property of Eucalyptus globulus Labill. Extracts and Inhibitory Activities on Carbohydrate Metabolizing Enzymes Related to Type-2 Diabetes10.1016/j.bcab.2021.102111 ↗
33. In Vitro and in Vivo Antioxidant Activity of Eucalyptus Leaf Polyphenols Extract and Its Effect on Chicken Meat Quality and Cecum Microbiota10.1016/j.foodres.2020.109302 ↗
34. Infusions from Thymus vulgaris L. Treated at Different Gamma Radiation Doses: Effects on Antioxidant Activity and Phenolic Composition10.1016/j.lwt.2016.07.027 ↗
35. Antioxidant Activity of Extracts from in Vitro Cultures of Salvia officinalis L.10.1016/j.foodchem.2006.12.003 ↗
36. Variation in the Phytochemical Contents and Antioxidant Activity of Glycyrrhiza glabra Populations Collected in Iran10.1016/j.indcrop.2019.05.034 ↗
37. Microencapsulação de Compostos Bioativos Pelo Método de Extrusão10.5902/2179460X19725 ↗
38. Antioxidant Edible Films Based on Chitosan and Starch Containing Polyphenols from Thyme Extracts10.1016/j.carbpol.2016.10.080 ↗
39. Enhancing the Functionality of Chitosan- and Alginate-Based Active Edible Coatings/Films for the Preservation of Fruits and Vegetables: A Review10.1016/j.ijbiomac.2020.07.083 ↗
40. Studies on Development and Evaluation of Glycerol Incorporated Cellulose and Alginate Based Edible Films10.5958/0974-4479.2017.00010.7 ↗
41. Characteristics of Ascorbic Acid Release from TPP-Crosslinked Chitosan/Alginate Polyelectrolyte Complex Membranes
42. Alginate Coatings Containing Grapefruit Essential Oil or Grapefruit Seed Extract for Grapes Preservation10.1111/ijfs.12387 ↗
43. Effect of Alginate Edible Coatings Enriched with Black Cumin Extract for Improving Postharvest Quality Characteristics of Guava (Psidium guajava L.) Fruit10.1007/s11947-022-02869-2 ↗
44. Edible Alginate-Based Coating as Carrier of Antimicrobials to Improve Shelf-Life and Safety of Fresh-Cut Melon10.1016/j.ijfoodmicro.2007.11.010 ↗
45. Effect of Citrus Wilsonii Tanaka Extract Combined with Alginate-Calcium Coating on Quality Maintenance of White Shrimps (Litopenaeus vannamei Boone)10.1016/j.foodcont.2016.03.028 ↗
46. Characterisation and Antibacterial Properties of Novel Biodegradable Films Based on Alginate and Roselle (Hibiscus sabdariffa L.) Extract10.1007/s12649-022-01710-3 ↗
47. Physical, Mechanical, and Bioactive Properties of Edible Film Based on Sodium Alginate Enriched with Lythrum salicaria L. Extract10.1111/jfpp.16620 ↗
48. Sadiq, M.B., Tarning, J., Cho, T.Z.A., and Anal, A.K. (2017). Activities and Possible Modes of Action of Acacia nilotica (L.) Del. against Multidrug-Resistant Escherichia coli and Salmonella. Molecules, 22.10.3390/molecules22010047 ↗
49. Chew, Y.L., Mahadi, A.M., Wong, K.M., and Goh, J.K. (2018). Anti-Methicillin-Resistance Staphylococcus Aureus (MRSA) Compounds from Bauhinia kockiana Korth. And Their Mechanism of Antibacterial Activity. BMC Complement. Altern. Med., 18.10.1186/s12906-018-2137-5 ↗
50. The Antimicrobial Capacity of Cistus salviifolius and Punica granatum Plant Extracts against Clinical Pathogens Is Related to Their Polyphenolic Composition10.1038/s41598-020-80003-y ↗
51. Zhou, D., Liu, Z.-H., Wang, D.-M., Li, D.-W., Yang, L.-N., and Wang, W. (2019). Chemical Composition, Antibacterial Activity and Related Mechanism of Valonia and Shell from Quercus variabilis Blume (Fagaceae) against Salmonella paratyphi a and Staphylococcus aureus. BMC Complement. Altern. Med., 19.10.1186/s12906-019-2690-6 ↗
52. Chemical Composition, Antibacterial Properties, and Mechanism of Smilax china L. Polyphenols10.1007/s00253-019-10100-0 ↗
53. Mombeshora, M., and Mukanganyama, S. (2019). Antibacterial Activities, Proposed Mode of Action and Cytotoxicity of Leaf Extracts from Triumfetta welwitschii against Pseudomonas aeruginosa. BMC Complement. Altern. Med., 19.10.1186/s12906-019-2713-3 ↗
54. Natural Products Show Diverse Mechanisms of Action against Clostridium difficile10.1111/jam.14152 ↗
55. Antimicrobial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms10.3389/fmicb.2018.01639 ↗
56. Characterization of Pea Starch-Guar Gum Biocomposite Edible Films Enriched by Natural Antimicrobial Agents for Active Food Packaging10.1016/j.fbp.2017.06.003 ↗
57. Luo, Y., Liu, H., Yang, S., Zeng, J., and Wu, Z. (2019). Sodium Alginate-Based Green Packaging Films Functionalized by Guava Leaf Extracts and Their Bioactivities. Materials, 12.10.3390/ma12182923 ↗
58. Development and Characterization of Alginate-Based Edible Film Incorporated with Hawthorn berry (Crataegus pinnatifida) Extract10.1007/s11694-021-00847-4 ↗
59. A Novel Sodium Alginate Active Films Functionalized with Purple Onion Peel Extract (Allium cepa)10.1016/j.bcab.2021.102096 ↗
60. Development and Characterization of an Antimicrobial Edible Film from Basil Seed (Ocimum basilicum L.) Mucilage and Sodium Alginate10.1016/j.bcab.2022.102450 ↗
61. Antimicrobial Activity of Chitosan-Based Films Enriched with Green Tea Extracts on Murine norovirus, Escherichia coli, and Listeria innocua10.1155/2020/3941924 ↗
62. Antifungal Activity of Biopolymers Containing Natamycin and Rosemary Extract against Aspergillus niger and Penicillium roquefortii10.1111/j.1365-2621.2008.01816.x ↗
63. Lozano-Navarro, J.I., Díaz-Zavala, N.P., Velasco-Santos, C., Martínez-Hernández, A.L., Tijerina-Ramos, B.I., García-Hernández, M., Rivera-Armenta, J.L., Páramo-García, U., and Reyes-de la Torre, A.I. (2017). Antimicrobial, Optical and Mechanical Properties of Chitosan–Starch Films with Natural Extracts. Int. J. Mol. Sci., 18.10.3390/ijms18050997 ↗
64. Antioxidant and Antimicrobial Properties of Ethylene Vinyl Alcohol Copolymer Films Based on the Release of Oregano Essential Oil and Green Tea Extract Components10.1016/j.jfoodeng.2014.10.007 ↗
65. Influence of Pitanga (Eugenia uniflora L.) Leaf Extract and/or Natamycin on Properties of Cassava Starch/Chitosan Active Films10.1016/j.fpsl.2020.100498 ↗
66. Properties and Antimicrobial Activities of Starch–Sodium Alginate Composite Films Incorporated with Sodium Dehydroacetate or Rosemary Extract10.1002/app.37570 ↗
67. Novel Composite Films Based on Sodium Alginate and Gallnut Extract with Enhanced Antioxidant, Antimicrobial, Barrier and Mechanical Properties10.1016/j.foodhyd.2020.106508 ↗
68. Valorization of Coffee Parchment Waste (Coffea arabica) as a Source of Caffeine and Phenolic Compounds in Antifungal Gellan Gum Films10.1016/j.lwt.2018.11.013 ↗
69. Comparative Studies on the Characterization and Antioxidant Properties of Biodegradable Alginate Films Containing Ginseng Extract10.1016/j.jfoodeng.2010.01.015 ↗
70. Effect of the Application of an Edible Film with Turmeric (Curcuma longa L.) on the Oxidative Stability of Meat10.1002/fsn3.1728 ↗
71. Antiviral and Antioxidant Properties of Active Alginate Edible Films Containing Phenolic Extracts10.1016/j.foodhyd.2018.02.026 ↗
72. Structural, Physicochemical and Antioxidant Properties of Sodium Alginate Isolated from a Tunisian Brown Seaweed10.1016/j.ijbiomac.2014.10.016 ↗
73. Physical Properties and Antioxidant Activity of Gelatin-Sodium Alginate Edible Films with Tea Polyphenols10.1016/j.ijbiomac.2018.06.121 ↗
74. Pink Pepper Phenolic Compounds Incorporation in Starch/Protein Blends and Its Potential to Inhibit Apple Browning10.1016/j.fpsl.2018.01.003 ↗