Antifungal Properties of Zinc Oxide Nanoparticles on Candida albicans
Source abstract
Abstract
This paper reports the antifungal properties of zinc oxide nanoparticles (ZnO NPs) on Candida albicans ATCC 1023 through the study of growth inhibitory effects of ZnO NPs on C. albicans and the effect of the nanoparticles on the surface of C. albicans. The growth inhibitory effects of ZnO NPs (5, 10, 20, 40, 80, and 160 µg/mL) on C. albicans at 24 h were determined through the reduction in suspension turbidity and colony count. Fourier transform infrared (FTIR) analysis was carried out to establish the functional groups associated with the interaction of ZnO NPs on the yeast cell wall, while scanning electron microscopy (SEM) with energy dispersive X-ray (SEM-EDX) analysis was utilised to determine the surface accumulation of ZnO NPs on the yeast cells and the consequential morphological alterations on C. albicans. The results exhibited a significant (p < 0.05) growth inhibition for all tested concentrations except for 5 µg/mL of ZnO NPs at 24 h as compared to negative control. FTIR analysis revealed the possible involvement of alcohol, amide A, methyl, alkynes, amide I and II, and phosphate groups from the cell wall of C. albicans in the surface interaction with ZnO NPs. Finally, SEM-EDX revealed a considerable accumulation of ZnO NPs on the yeast cells and consequential morphological alterations on C. albicans, including the damage of hyphae, pitting of the cell wall, invagination, and rupture of the cell membrane. The current study demonstrated that ZnO NPs possess antifungal properties against C. albicans in a dose-dependent manner, and the surface interaction of ZnO NPs on fungal cells caused alterations in cell membrane integrity that might have resulted in cell death.
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Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Nanocomposite Films for Food Packaging · Dielectric properties of ceramics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biomedical Science, Universiti Tunku Abdul Rahman (UTAR), Kampar 31900, Malaysia
Life Science Division, Faculty of Health and Life Sciences, INTI International University, Nilai 71800, Malaysia
Viyen Biotech LLP, Coimbatore 641034, Tamil Nadu, India
Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore 641028, Tamil Nadu, India
Faculty of Bioeconomics and Health sciences, Geomatika University College, Kuala Lumpur 54200, Malaysia
School of Applied Sciences, Faculty of Engineering, Science & Technology, Nilai University, Nilai 71800, Malaysia
Department of Chemical Sciences, Universiti Tunku Abdul Rahman, Kampar 31900, Malaysia
Faculty of Pharmaceutical Sciences, UCSI University, Cheras 56000, Malaysia
Centre for Photonics and Advanced Materials Research (CPAMR), Universiti Tunku Abdul Rahman (UTAR), Sungai Long Campus, Jalan Sungai Long, Bandar Sungai Long, 43000 Kajang, Malaysia
Faculty of Dentistry, AIMST University, Bedong, Kedah 08100, Malaysia
Funding
Universiti Tunku Abdul Rahman Research Fund · Ministry of Higher Education (MOHE), Malaysia
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