Synthesis and characterization of mesoporous silica SBA 15 improved the efficacy of CORM-2 against hypoxia reoxygenation injury

Carbon monoxide releasing molecule-2 (CORM-2) exhibited protection against cardiac hypoxia reoxygenation injury (H/R). However, its poor water solubility and short half-life restrict its clinical usage. The present study aims to investigate whether encapsulation of CORM-2 in mesoporous silica (MSN-A-CORM-2) can enhance its anti-H/R effect in cardiac cell lines H9C2 (cardiomyoblast) and 3T3 (fibroblast). Mesoporous silica nanoparticles (MSN) were synthesized by co-operative self-assembly technique through solvothermal strategy and terminated with amine functional groups (MSN-A). The ligand, CORM-2, was linked to the terminal amine group in mesoporous silica. The physicochemical characterization of the compound was made by using SEM, TEM, FTIR, and EDS analysis. Further, we evaluated the biological effect of MSN-A-CORM-2 against H/R in H9C2 and 3T3 cells. The results demonstrated that MSN-A-CORM-2 was less toxic as compared to that of standard CORM-2. The comparable potential of CORM-2 in combating H/R got enhanced upon encapsulation with mesoporous silica.