Computational Microfluidic Channel for Separation of Escherichia coli from Blood-Cells
Source abstract
Abstract
Microfluidic channels play a vital role in separation of analytes of interest such as bacteria and platelet cells, etc., in various biochemical diagnosis procedures including urinary tract infections (UTI) and bloodstream infections. This paper presents the multi physics computational model specifically designed to study the effects of design parameters of a microfluidics channel for the separation of Escherichia coli (E. coli) from various blood constituents including red blood cells (RBC) and platelets. A standard two inlet and a two outlet microchannel of length 805 m with a channel width of 40 m is simulated. The effect of electrode potentials and the effect of electrode placement along the channel length and also the levitation of electrodes from the channel wall are studied to optimize the selective particle separation throughput. Simulated results show the efficient separation of E-coli with a mean diameter 0.68 m is achieved at low voltages (less than 20 V) when electrodes placed near to the micro channel and also noticed that the applied electric potential is inversely proportional to the number of electrodes placed along the microfluidic channel. The computer aided multi physics simulations with multiple governing parameters could be advantage in design optimization of microfluidics channels and support precise bioparticle separation for better diagnosis.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Microfluidic and Bio-sensing Technologies · Microfluidic and Capillary Electrophoresis Applications · Electrostatics and Colloid Interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (46)
Show source-provided references
1. A simple microfluidic chip design for fundamental bioseparation10.1155/2014/175457 ↗
2. Efficient and rapid detection of salmonella using microfluidic impedance based sensing
3. Recent advances in lab-on-a-chip for biosensing applications10.1016/j.bios.2015.08.003 ↗
4. Introduction to micro-analytical systems: Bioanalytical and pharmaceutical applications10.1016/S0928-0987(03)00147-7 ↗
5. The future of microfluidic point-of-care diagnostic devices10.4155/bio.12.307 ↗
6. Pinched flow fractionation: Continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel10.1021/ac049863r ↗
7. A numerical simulation of cell separation by simplified asymmetric pinched flow fractionation10.1155/2016/2564584 ↗
8. Progress of inertial microfluidics in principle and application10.3390/s18061762 ↗
9. A numbering-up strategy of hydrodynamic microfluidic filters for continuous-flow high-throughput cell sorting10.1039/C9LC00053D ↗
10. Hydrodynamic trapping of particles in an expansion-contraction microfluidic device10.1155/2013/496243 ↗
11. Parameter screening in microfluidics based hydrodynamic single-cell trapping10.1155/2014/929163 ↗
12. Design rules for size-based cell sorting and sheathless cell focusing by hydrophoresis10.1016/j.chroma.2013.06.030 ↗
13. A review on deterministic lateral displacement for particle separation and detection10.1007/s40820-019-0308-7 ↗
14. Study on microchannel design and burst frequency detection for centrifugal microfluidic system10.1155/2013/137347 ↗
15. Particle sorting using a porous membrane in a microfluidic device10.1039/C0LC00121J ↗
16. W. Lee, P. Tseng and D. D. Carlo, Microtechnology for cell manipulation and sorting, 1$\rm st$st ed., New York: Springer International Publishing, pp. 57–92, 2017.10.1007/978-3-319-44139-9 ↗
17. Fundamentals and application of magnetic particles in cell isolation and enrichment: A review10.1088/0034-4885/78/1/016601 ↗
18. Acoustofluidic separation of cells and particles10.1038/s41378-019-0064-3 ↗
19. Sorting and manipulation of biological cells and the prospects for using optical forces10.1186/s40486-018-0064-3 ↗
20. A review of microfabrication techniques and dielectrophoretic microdevices for particle manipulation and separation10.1088/0022-3727/47/6/063001 ↗
21. Dielectrophoresis: Status of the theory, technology, and applications
22. Computational fluid dynamics modelling of microfluidic channel for dielectrophoretic biomems application10.1155/2014/961301 ↗
23. Dielectrophoretic separation of platelets from diluted whole blood in microfluidic channels10.1002/elps.200700607 ↗
24. Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation10.1063/1.3640045 ↗
25. AC electrokinetic induced non-Newtonian electrothermal blood flow in 3D microfluidic biosensor with ring electrodes for point-of-care diagnostics10.1063/1.5099272 ↗
26. AC electrothermal effect in microfluidics: A review10.3390/mi10110762 ↗
27. Dielectrophoretic separation of platelet cells in a microfluidic channel and optimization with fuzzy logic
28. A new feature selection method based on machine learning technique for air quality dataset10.1080/09720510.2019.1609726 ↗
29. Detection of focal and non-focal electroencephalogram signals using fast walsh-hadamard transform and artificial neural network
30. Voice pathology detection and classification using convolutional neural network model10.3390/app10113723 ↗
31. Decision-level fusion scheme for nasopharyngeal carcinoma identification using machine learning techniques10.1007/s00521-018-3882-6 ↗
32. Evaluating the performance of machine learning techniques in the classification of Wisconsin breast cancer10.14419/ijet.v7i4.36.23737 ↗
33. Modified single pass clustering with variable threshold approach
34. Dielectrophoresis for biomedical sciences applications: A review
35. Li · Encyclopedia of Microfluidics and Nanofluidics · 2008 · 402
36. AC-dielectrophoretic characterization and separation of submicron and micron particles using sidewall AgPDMS electrodes10.1063/1.3682049 ↗
37. T. B. Jones, “Electro mechanics of particles,” 1$\rm st$st ed. Cambridge: Cambridge University Press, pp. 34–82, 2005.
38. R. R. Pethig, “Dielectrophoresis theory, methodology and biological applications,” 1$\rm st$st ed. Hoboken: John Wiley & Sons Ltd., pp. 31–141, 2017.10.1002/9781118671443 ↗
39. MyDEP: A new computational tool for dielectric modeling of particles and cells10.1016/j.bpj.2018.11.021 ↗
40. Y. A. Cengel and J. M. Cimbala, “Fluid mechanics: Fundamentals and applications introduction and basic concepts,” 4$\rm th$th ed. New York: McGraw-Hill College, pp. 611–650, 2017.
41. A simple pneumatic setup for driving microfluidics10.1039/B617673A ↗
42. Characterization and optimization of liquid electrodes for lateral dielectrophoresis10.1039/B612866A ↗
43. Electrode design for negative dielectrophoresis10.1088/0957-0233/2/12/005 ↗
44. Electrorotation measurements of diamide-induced platelet activation changes10.1016/S0006-3495(95)80197-9 ↗
45. Characterization of simple and double yeast cells using dielectrophoretic force measurement
46. Jones · Analytical and Bioanalytical Chemistry · 2014 · 406 · 18310.1007/s00216-013-7437-5 ↗