DataVXChemNanoMatPaper
journal article·2016-09

Replication of Polymer‐Based Peptide Microarrays by Multi‐Step Transfer

Jakob Striffler, Daniela S. Mattes, Sebastian Schillo, Bastian Münster, Andrea Palermo, Barbara Ridder, Alexander Welle, Vanessa Trouillet, Volker Stadler, Goran Markovic, Günther Proll, Stefan Bräse, Felix F. Loeffler, Alexander Nesterov‐Müller, Frank Breitling

ChemNanoMat· Volume 2· Issue 9
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Source abstract

Abstract

Abstract Peptide microarrays serve as a high‐throughput tool for the readout of protein–peptide interactions. We present a multi‐step purification of high‐complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times.
Source: crossref · Quality: baseline

Research context

Polymers

OpenAlex topics: Advanced Biosensing Techniques and Applications · Advanced biosensing and bioanalysis techniques · Chemical Synthesis and Analysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Microstructure Technology (IMT) Karlsruhe Institute of Technology Hermann-von-Helmholtz-Platz 1 76344 Eggenstein-Leopoldshafen Germany

Institute of Organic Chemistry Karlsruhe Institute of Technology (KIT) Fritz-Haber-Weg 6 76131 Karlsruhe Germany

Institute of Functional Interfaces (IFG) and Karlsruhe Nano Micro Facility (KMNF) Karlsruhe Institute of Technology Hermann-von-Helmholtz-Platz 1 76344 Eggenstein-Leopoldshafen Germany

Institute for Applied Materials (IAM) and Karlsruhe Nano Micro Facility (KMNF) Karlsruhe Institute of Technology Hermann-von-Helmholtz-Platz 1 76344 Eggenstein-Leopoldshafen Germany

PEPperPRINT GmbH Rischerstraße 12 69123 Heidelberg Germany

Eberhard Karls University Auf der Morgenstelle 18 72076 Tübingen Germany

Institute of Toxicology and Genetics Karlsruhe Institute of Technology (KIT) Hermann-von-Helmholtz-Platz 1 76344 Eggenstein-Leopoldshafen Germany

Funding

KNMF · ERC St · HRJRG · Carl-Zeiss-Stiftung · BMBF KATMETHAN · EU FP7 PEPDIODE · EU FP7 TARGETBINDER

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