Construction of Pillar[5]arene‐based Nanochannels with Chiral‐enhanced DNA Translocation Ability

Abstract Chirality is an intrinsic feature of nature and plays a crucial role in molecular translocation. However, how chirality affects or even more strongly affects DNA translocation remains largely unknown. Here, a facile and feasible strategy was contributed for the construction of chiral pillar[5]arene‐modified nanochannels (D/L‐Phe‐P5 nanochannels) that can enhance recognition and accelerate DNA translocation. The results showed the L‐Phe‐P5 nanochannel elevated the DNA translocation efficiency by about 6.59‐fold compared to the Mono‐L‐Phe‐P5 channel. Furthermore, the translocation efficiency of the L‐Phe‐P5 nanochannel with multi‐chiral centers was 3.06‐fold higher than that of the D‐Phe‐P5 nanochannel, and this value was 2.25‐fold higher than the ratio of the translocation efficiency between the Mono‐L‐Phe‐P5 channel and Mono‐D‐Phe‐P5 channel. Finally, the theoretical simulation and analysis of the translocation mechanism were performed by COMSOL Multiphysics software. This research not only enriches the basic theory research related to the influence of chirality on DNA translocation, but also has great reference value for the further development of DNA application. More importantly, the chiral amplification strategy could also extend to the selectivity of other chiral biomolecules, which may have potentially far‐reaching implications.