The Effects of Electric Fields on Protein Phase Behavior and Protein Crystallization Kinetics
Source abstract
Abstract
We experimentally studied the effects of an externally applied electric field on protein crystallization and liquid-liquid phase separation (LLPS) and its crystallization kinetics. For a surprisingly weak alternating current (AC) electric field, crystallization was found to occur in a wider region of the phase diagram, while nucleation induction times were reduced, and crystal growth rates were enhanced. LLPS on the contrary was suppressed, which diminishes the tendency for a two-step crystallization scenario. The effect of the electric field is ascribed to a change in the protein-protein interaction potential.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Enzyme Structure and Function · Crystallization and Solubility Studies · Proteins in Food Systems
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Biological Information Processing IBI-4, Forschungszentrum Jülich, 52428, Jülich, Germany
Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India
Faculty of Mathematics and Natural Sciences, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
Funding
Deutsche Forschungsgemeinschaft
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