Metallic Cation-Mediated Entrapment of Nucleic Acids on Mesoporous Silica Surface: Application in Castration-Resistant Prostate Cancer

Angelea Maestas-Olguin; Marian Olewine; Sheymah Thabata; Johanna Tsala Ebode; Mariella Arcos; Jacob Krawchuck; Eric N. Coker; Adrian J. Brearley; Xiang Xue; John Watt; Achraf Noureddine; C. Jeffrey Brinker

doi:10.1021/acs.chemmater.3c01174

journal article·2023-12-26Open access

Metallic Cation-Mediated Entrapment of Nucleic Acids on Mesoporous Silica Surface: Application in Castration-Resistant Prostate Cancer

Angelea Maestas-Olguin, Marian Olewine, Sheymah Thabata, Johanna Tsala Ebode, Mariella Arcos, Jacob Krawchuck, Eric N. Coker, Adrian J. Brearley, Xiang Xue, John Watt, Achraf Noureddine, C. Jeffrey Brinker

Chemistry of Materials· Volume 35· Issue 24

Publisher page Open full text

Source abstract

Abstract

The use of exogenous nucleic acid technologies to modulate aberrant protein expression resulting from genetic mutations is a promising therapeutic approach for the treatment of diseases such as advanced prostate cancer (PC). The promise of nucleic-based therapeutics is dependent on the development of platforms that effectively protect nucleic acids from nuclease degradation and deliver the nucleic acids to the cytosol of target cells. In this work, we present the development of a divalent metal-mediated nucleic acid entrapment strategy with a porous silica matrix. This simple strategy results in efficient loading percentages of both siRNA (>60%) and mRNA (>80%) as well as their release within relevant biological environments (80%). Additionally, our data supports that the current method reduces endosomal entrapment and supports the lipid coating of mesoporous silica nanoparticles (LC-MSNs). The metal-enhanced nanosystem is assessed for biocompatibility, stability, and circulation within in vitro, ex ovo, and in vivo models of PC.

Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials Biomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Nanoparticle-Based Drug Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, New Mexico 87131, United States

Department of Biochemistry School of Medicine, University of New Mexico, Albuquerque, New Mexico 87131, United States

Department of Biochemistry and Molecular Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States

Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, New Mexico 87123, United States

Department of Applied Optical/Plasma Sciences, Sandia National Laboratories, Albuquerque, New Mexico 87123, United States

Department of Earth and Planetary Sciences, University of New Mexico, Albuquerque, New Mexico 87131, United States

Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, United States

Funding

National Cancer Institute · Division of Graduate Education · University of New Mexico · National Institute of General Medical Sciences

References (41)

Show source-provided references

1. 10.1038/s41391-017-0023-810.1038/s41391-017-0023-8 ↗

2. 10.1016/j.cell.2018.05.03610.1016/j.cell.2018.05.036 ↗

3. 10.1016/j.ccr.2005.10.00110.1016/j.ccr.2005.10.001 ↗

4. 10.1016/j.ccr.2014.06.01510.1016/j.ccr.2014.06.015 ↗

5. 10.1016/j.tem.2010.01.00210.1016/j.tem.2010.01.002 ↗

6. 10.1016/j.cell.2018.05.03710.1016/j.cell.2018.05.037 ↗

7. 10.1016/j.ccr.2004.07.00810.1016/j.ccr.2004.07.008 ↗

8. 10.1016/j.addr.2022.11411310.1016/j.addr.2022.114113 ↗

9. 10.1038/s41392-022-01007-w10.1038/s41392-022-01007-w ↗

10. 10.1016/j.addr.2017.07.02110.1016/j.addr.2017.07.021 ↗

11. 10.1016/j.addr.2020.06.00210.1016/j.addr.2020.06.002 ↗

12. 10.1038/s41565-019-0591-y10.1038/s41565-019-0591-y ↗

13. 10.1038/s41578-021-00358-010.1038/s41578-021-00358-0 ↗

14. 10.1021/acsnano.1c0499610.1021/acsnano.1c04996 ↗

15. 10.1021/acs.accounts.9b0036810.1021/acs.accounts.9b00368 ↗

16. 10.1016/j.addr.2012.09.03710.1016/j.addr.2012.09.037 ↗

17. 10.1016/j.actbio.2020.07.02710.1016/j.actbio.2020.07.027 ↗

18. 10.1002/adhm.20170083110.1002/adhm.201700831 ↗

19. 10.1021/acs.accounts.0c0028010.1021/acs.accounts.0c00280 ↗

20. 10.1002/adfm.20190953910.1002/adfm.201909539 ↗

21. 10.1021/acsnano.3c0762110.1021/acsnano.3c07621 ↗

22. 10.1038/s41598-023-33092-410.1038/s41598-023-33092-4 ↗

23. 10.1002/smll.20150211910.1002/smll.201502119 ↗

24. 10.1039/c4ra00781f10.1039/c4ra00781f ↗

25. 10.1002/smll.20120002810.1002/smll.201200028 ↗

26. 10.1021/nn900918w10.1021/nn900918w ↗

27. 10.1016/j.biomaterials.2012.10.07210.1016/j.biomaterials.2012.10.072 ↗

28. 10.1021/acsami.9b0447410.1021/acsami.9b04474 ↗

29. 10.1021/acsami.8b0382310.1021/acsami.8b03823 ↗

30. 10.1021/acsami.8b0908610.1021/acsami.8b09086 ↗

31. 10.1021/acs.chemrev.1c0024410.1021/acs.chemrev.1c00244 ↗

32. 10.1002/adma.20160063410.1002/adma.201600634 ↗

33. 10.1007/s10971-014-3321-910.1007/s10971-014-3321-9 ↗

34. 10.1021/acsnano.6b0281910.1021/acsnano.6b02819 ↗

35. 10.1007/s10971-018-4772-110.1007/s10971-018-4772-1 ↗

36. 10.1021/acs.accounts.8b0029210.1021/acs.accounts.8b00292 ↗

37. 10.1146/annurev-physiol-012110-14231710.1146/annurev-physiol-012110-142317 ↗

38. 10.1038/nbt77410.1038/nbt774 ↗

39. 10.1016/j.ccr.2004.08.01810.1016/j.ccr.2004.08.018 ↗

40. 10.1021/acsnano.2c0399010.1021/acsnano.2c03990 ↗

41. 10.3389/fimmu.2021.79108110.3389/fimmu.2021.791081 ↗