Recent Advancements on Three-Dimensional Electrospun Nanofiber Scaffolds for Tissue Engineering
Source abstract
Abstract
Electrospinning is widely accepted as a technique for the fabrication of nanofibrous three-dimensional (3D) scaffolds which mimic extracellular matrix (ECM) microenvironment for tissue engineering (TE). Unlike normal densely-packed two-dimensional (2D) nanofibrous membranes, 3D electrospun nanofiber scaffolds are dedicated to more precise spatial control, endowing the scaffolds with a sufficient porosity and 3D environment similar to the in vivo settings as well as optimizing the properties, including injectability, compressibility, and bioactivity. Moreover, the 3D morphology regulates cellular interaction and mediates growth, migration, and differentiation of cell for matrix remodeling. The variation among scaffold structures, functions and applications depends on the selection of electrospinning materials and methods as well as on the post-processing of electrospun scaffolds. This review summarizes the recent new forms for building electrospun 3D nanofiber scaffolds for TE applications. A variety of approaches aimed at the fabrication of 3D electrospun scaffolds, such as multilayering electrospinning, sacrificial agent electrospinning, wet electrospinning, ultrasound-enhanced electrospinning as well as post-processing techniques, including gas foaming, ultrasonication, short fiber assembly, 3D printing, electrospraying, and so on are discussed, along with their advantages, limitations and applications. Meanwhile, the current challenges and prospects of 3D electrospun scaffolds are rationally discussed, providing an insight into developing the vibrant fields of biomedicine.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Tissue Engineering and Regenerative Medicine · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (100+)
Show source-provided references
1. Dvir T, Timko BP, Kohane DS, Langer R. Nanotechnological strategies for engineering complex tissues. Nat Nanotechnol 2011;6:13–22. https://doi.org/10.1038/nnano.2010.246 .10.1038/nnano.2010.246 ↗
2. Cheng J, Jun Y, Qin J, Lee SH. Electrospinning versus microfluidic spinning of functional fibers for biomedical applications. Biomaterials 2017;114:121–43. https://doi.org/10.1016/j.biomaterials.2016.10.040 .10.1016/j.biomaterials.2016.10.040 ↗
3. Wan X, Zhao Y, Li Z, Li L. Emerging polymeric electrospun fibers: from structural diversity to application in flexible bioelectronics and tissue engineering. Exploration 2022;2:20210029. https://doi.org/10.1002/exp.20210029 .10.1002/exp.20210029 ↗
4. Matson JB, Zha RH, Stupp SI. Peptide self-assembly for crafting functional biological materials. Curr Opin Solid State Mater Sci 2011;15:225–35. https://doi.org/10.1016/j.cossms.2011.08.001 .10.1016/j.cossms.2011.08.001 ↗
5. Holzwarth JM, Ma PX. Biomimetic nanofibrous scaffolds for bone tissue engineering. Biomaterials 2011;32:9622–9. https://doi.org/10.1016/j.biomaterials.2011.09.009 .10.1016/j.biomaterials.2011.09.009 ↗
6. Wade RJ, Burdick JA. Engineering ECM signals into biomaterials. Mater Today 2012;15:454–9. https://doi.org/10.1016/S1369-7021(12)70197-9 .10.1016/S1369-7021(12)70197-9 ↗
7. Khajavi R, Abbasipour M, Bahador A. Electrospun biodegradable nanofibers scaffolds for bone tissue engineering. J Appl Polym Sci 2016;133:1–19. https://doi.org/10.1002/app.42883 .10.1002/app.42883 ↗
8. Greiner A, Wendorff JH. Electrospinning: a fascinating method for the preparation of ultrathin fibers. Angew Chem Int Ed 2007;46:5670–703. https://doi.org/10.1002/anie.200604646 .10.1002/anie.200604646 ↗
9. Wang X, Ding B, Li B. Biomimetic electrospun nanofibrous structures for tissue engineering. Mater Today 2013;16:229–41. https://doi.org/10.1016/j.mattod.2013.06.005 .10.1016/j.mattod.2013.06.005 ↗
10. Li D, Xia Y. Electrospinning of nanofibers: Reinventing the wheel? Adv Mater 2004;16:1151–70. https://doi.org/10.1002/adma.200400719 .10.1002/adma.200400719 ↗
11. Abd Razak SI, Wahab IF, Fadil F, Dahli FN, Md Khudzari AZ, Adeli H. A review of electrospun conductive polyaniline based nanofiber composites and blends: processing features, applications, and future directions. Adv Mater Sci Eng. 2015. https://doi.org/10.1155/2015/356286.10.1155/2015/356286 ↗
12. Xue J, Xie J, Liu W, Xia Y. Electrospun nanofibers: new concepts, materials, and applications. Acc Chem Res 2017;50:1976–87. https://doi.org/10.1021/acs.accounts.7b00218 .10.1021/acs.accounts.7b00218 ↗
13. Chen Y, Sha M, Liu M, Morsi Y, Mo X. Advanced fabrication for electrospun three-dimensional nanofiber aerogels and scaffolds. Bioact Mater 2020;5:963–79. https://doi.org/10.1016/j.bioactmat.2020.06.023 .10.1016/j.bioactmat.2020.06.023 ↗
14. Liu W, Thomopoulos S, Xia Y. Electrospun nanofibers for regenerative medicine. Adv Healthc Mater 2012;1:10–25. https://doi.org/10.1002/adhm.201100021 .10.1002/adhm.201100021 ↗
15. Frantz C, Stewart KM, Weaver VM. The extracellular matrix at a glance. J Cell Sci 2010;123:4195–200. https://doi.org/10.1242/jcs.023820 .10.1242/jcs.023820 ↗
16. Bancelin S, Aimé C, Gusachenko I, Kowalczuk L, Latour G, Coradin T, Schanne-Klein MC. Determination of collagen fibril size via absolute measurements of second-harmonic generation signals. Nat Commun 2014;5:1–8. https://doi.org/10.1038/ncomms5920 .10.1038/ncomms5920 ↗
17. Agarwal S, Wendorff JH, Greiner A. Progress in the field of electrospinning for tissue engineering applications. Adv Mater 2009;21:3343–51. https://doi.org/10.1002/adma.200803092 .10.1002/adma.200803092 ↗
18. Aamodt JM, Grainger DW. Extracellular matrix-based biomaterial scaffolds and the host response. Biomaterials 2016;86:68–82. https://doi.org/10.1016/j.biomaterials.2016.02.003 .10.1016/j.biomaterials.2016.02.003 ↗
19. Du J, Yarema KJ. Carbohydrate engineered cells for regenerative medicine. Adv Drug Deliv Rev 2010;62:671–82. https://doi.org/10.1016/j.addr.2010.01.003 .10.1016/j.addr.2010.01.003 ↗
20. Xie X, Chen Y, Wang X, Xu X, Shen Y, Aldalbahi A, Fetz AE, Bowlin GL, El-Newehy M, Mo X. Electrospinning nanofiber scaffolds for soft and hard tissue regeneration. J Mater Sci Technol. 2020;59:243–61. https://doi.org/10.1016/j.jmst.2020.04.037.10.1016/j.jmst.2020.04.037 ↗
21. Dutta RC, Dey M, Dutta AK, Basu B. Competent processing techniques for scaffolds in tissue engineering. Biotechnol Adv 2017;35:240–50. https://doi.org/10.1016/j.biotechadv.2017.01.001 .10.1016/j.biotechadv.2017.01.001 ↗
22. Tang X, Thankappan SK, Lee P, Fard SE, Harmon MD, Tran K, Yu X. Polymeric biomaterials in tissue engineering and regenerative medicine. Elsevier Inc.; 2014. https://doi.org/10.1016/B978-0-12-396983-5.00022-3.10.1016/B978-0-12-396983-5.00022-3 ↗
23. Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ. Electrospun collagen-chitosan nanofiber: a biomimetic extracellular matrix for endothelial cell and smooth muscle cell. Acta Biomater 2010;6:372–82. https://doi.org/10.1016/j.actbio.2009.07.024 .10.1016/j.actbio.2009.07.024 ↗
24. Brenner EK, Schiffman JD, Thompson EA, Toth LJ, Schauer CL. Electrospinning of hyaluronic acid nanofibers from aqueous ammonium solutions. Carbohydr Polym 2012;87:926–9. https://doi.org/10.1016/j.carbpol.2011.07.033 .10.1016/j.carbpol.2011.07.033 ↗
25. Jin D, Hu J, Xia D, Liu A, Kuang H, Du J, Mo X, Yin M. Evaluation of a simple off-the-shelf bi-layered vascular scaffold based on poly(L-lactide-co-ε-caprolactone)/silk fibroin in vitro and in vivo. Int J Nanomed 2019;14:4261–76. https://doi.org/10.2147/IJN.S205569 .10.2147/IJN.S205569 ↗
26. Wu T, Zheng H, Chen J, Wang Y, Sun B, Morsi Y, El-Hamshary H, Al-Deyab SS, Chen C, Mo X. Application of a bilayer tubular scaffold based on electrospun poly(l-lactide-co-caprolactone)/collagen fibers and yarns for tracheal tissue engineering. J Mater Chem B 2017;5:139–50. https://doi.org/10.1039/c6tb02484j .10.1039/c6tb02484j ↗
27. Panzavolta S, Gioffrè M, Focarete ML, Gualandi C, Foroni L, Bigi A. Electrospun gelatin nanofibers: optimization of genipin cross-linking to preserve fiber morphology after exposure to water. Acta Biomater 2011;7:1702–9. https://doi.org/10.1016/j.actbio.2010.11.021 .10.1016/j.actbio.2010.11.021 ↗
28. Hussey GS, Dziki JL, Badylak SF. Extracellular matrix-based materials for regenerative medicine. Nat Rev Mater 2018;3:159–73. https://doi.org/10.1038/s41578-018-0023-x .10.1038/s41578-018-0023-x ↗
29. Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 2003;63:2223–53. https://doi.org/10.1016/S0266-3538(03)00178-7 .10.1016/S0266-3538(03)00178-7 ↗
30. Zhou T, Wang N, Xue Y, Ding T, Liu X, Mo X, Sun J. Electrospun tilapia collagen nanofibers accelerating wound healing via inducing keratinocytes proliferation and differentiation. Colloids Surf B Biointerfaces 2016;143:415–22. https://doi.org/10.1016/j.colsurfb.2016.03.052 .10.1016/j.colsurfb.2016.03.052 ↗
31. Pang Y, Qin A, Lin X, Yang L, Wang Q, Wang Z, Shan Z, Li S, Wang J, Fan S, Hu Q. Oncotarget 35583 www.impactjournals.com/oncotarget Biodegradable and biocompatible high elastic chitosan scaffold is cell-friendly both in vitro and in vivo. Oncotarget. 2017;8:35583–91.10.18632/oncotarget.14709 ↗
32. Gurumurthy B, Griggs JA, Janorkar AV. Optimization of collagen-elastin-like polypeptide composite tissue engineering scaffolds using response surface methodology. J Mech Behav Biomed Mater 2018;84:116–25. https://doi.org/10.1016/j.jmbbm.2018.04.019 .10.1016/j.jmbbm.2018.04.019 ↗
33. Rad LR, Momeni A, Ghazani BF, Irani M, Mahmoudi M, Noghreh B. Removal of Ni2+ and Cd2+ ions from aqueous solutions using electrospun PVA/zeolite nanofibrous adsorbent. Chem Eng J 2014;256:119–27. https://doi.org/10.1016/j.cej.2014.06.066 .10.1016/j.cej.2014.06.066 ↗
34. Ma J, He Y, Liu X, Chen W, Wang A, Lin CY, Mo X, Ye X. A novel electrospun-aligned nanoyarn/three-dimensional porous nanofibrous hybrid scaffold for annulus fibrosus tissue engineering. Int J Nanomed 2018;13:1553–67. https://doi.org/10.2147/IJN.S143990 .10.2147/IJN.S143990 ↗
35. Zhang K, Fu Q, Yoo J, Chen X, Chandra P, Mo X, Song L, Atala A, Zhao W. 3D bioprinting of urethra with PCL/PLCL blend and dual autologous cells in fibrin hydrogel: an in vitro evaluation of biomimetic mechanical property and cell growth environment. Acta Biomater 2017;50:154–64. https://doi.org/10.1016/j.actbio.2016.12.008 .10.1016/j.actbio.2016.12.008 ↗
36. Kuang H, Wang Y, Hu J, Wang C, Lu S, Mo X. A method for preparation of an internal layer of artificial vascular graft co-modified with salvianolic acid B and heparin. ACS Appl Mater Interfaces 2018;10:19365–72. https://doi.org/10.1021/acsami.8b02602 .10.1021/acsami.8b02602 ↗
37. Rinoldi C, Kijeńska E, Chlanda A, Choinska E, Khenoussi N, Tamayol A, Khademhosseini A, Swieszkowski W. Nanobead-on-string composites for tendon tissue engineering. J Mater Chem B 2018;6:3116–27. https://doi.org/10.1039/c8tb00246k .10.1039/c8tb00246k ↗
38. Xue J, Wu T, Dai Y, Xia Y. Electrospinning and electrospun nanofibers: methods, materials, and applications. Chem Rev 2019;119:5298–415. https://doi.org/10.1021/acs.chemrev.8b00593 .10.1021/acs.chemrev.8b00593 ↗
39. Huang W, Xiao Y, Shi X. Construction of electrospun organic/inorganic hybrid nanofibers for drug delivery and tissue engineering applications. Adv Fiber Mater 2019;1:32–45. https://doi.org/10.1007/s42765-019-00007-w .10.1007/s42765-019-00007-w ↗
40. Heuer-Jungemann A, Feliu N, Bakaimi I, Hamaly M, Alkilany A, Chakraborty I, Masood A, Casula MF, Kostopoulou A, Oh E, Susumu K, Stewart MH, Medintz IL, Stratakis E, Parak WJ, Kanaras AG. The role of ligands in the chemical synthesis and applications of inorganic nanoparticles. Chem Rev 2019;119:4819–80. https://doi.org/10.1021/acs.chemrev.8b00733 .10.1021/acs.chemrev.8b00733 ↗
41. Bose S, Tarafder S. Calcium phosphate ceramic systems in growth factor and drug delivery for bone tissue engineering: a review. Acta Biomater 2012;8:1401–21. https://doi.org/10.1016/j.actbio.2011.11.017 .10.1016/j.actbio.2011.11.017 ↗
42. Hossain KMZ, Patel U, Ahmed I. Development of microspheres for biomedical applications: a review. Prog Biomater 2015;4:1–19. https://doi.org/10.1007/s40204-014-0033-8 .10.1007/s40204-014-0033-8 ↗
43. Denry I, Kuhn LT. Design and characterization of calcium phosphate ceramic scaffolds for bone tissue engineering. Dent Mater 2016;32:43–53. https://doi.org/10.1016/j.dental.2015.09.008 .10.1016/j.dental.2015.09.008 ↗
44. Poologasundarampillai G, Wang D, Li S, Nakamura J, Bradley R, Lee PD, Stevens MM, McPhail DS, Kasuga T, Jones JR. Cotton-wool-like bioactive glasses for bone regeneration. Acta Biomater 2014;10:3733–46. https://doi.org/10.1016/j.actbio.2014.05.020 .10.1016/j.actbio.2014.05.020 ↗
45. Liang R, Zhao J, Li B, Cai P, Loh XJ, Xu C, Chen P, Kai D, Zheng L. Implantable and degradable antioxidant poly(ε-caprolactone)-lignin nanofiber membrane for effective osteoarthritis treatment. Biomaterials 2020;230:119601. https://doi.org/10.1016/j.biomaterials.2019.119601 .10.1016/j.biomaterials.2019.119601 ↗
46. Ponnusamy VK, Nguyen DD, Dharmaraja J, Shobana S, Banu JR, Saratale RG, Chang SW, Kumar G. A review on lignin structure, pretreatments, fermentation reactions and biorefinery potential. Bioresour Technol 2019;271:462–72. https://doi.org/10.1016/j.biortech.2018.09.070 .10.1016/j.biortech.2018.09.070 ↗
47. Wang C, Kelley SS, Venditti RA. Lignin-based thermoplastic materials. Chemsuschem 2016;9:770–83. https://doi.org/10.1002/cssc.201501531 .10.1002/cssc.201501531 ↗
48. Wang Z, Lin M, Xie Q, Sun H, Huang Y, Zhang DD, Yu Z, Bi X, Chen J, Wang J, Shi W, Gu P, Fan X. Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration. Int J Nanomed 2016;11:1483–500. https://doi.org/10.2147/IJN.S97445 .10.2147/IJN.S97445 ↗
49. Jin HJ, Chen J, Karageorgiou V, Altman GH, Kaplan DL. Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 2004;25:1039–47. https://doi.org/10.1016/S0142-9612(03)00609-4 .10.1016/S0142-9612(03)00609-4 ↗
50. Cai K, Yao K, Lin S, Yang Z, Li X. Poly(D,L-lactic acid) surfaces modified by silk fibroin: effects on the culture of osteoblast in vitro. Biomaterals. 2002;23:1153–60.10.1016/S0142-9612(01)00230-7 ↗
51. Sun B, Zhou Z, Li D, Wu T, Zheng H, Liu J, Wang G, Yu Y, Mo X. Polypyrrole-coated poly(l-lactic acid-co-ε-caprolactone)/silk fibroin nanofibrous nerve guidance conduit induced nerve regeneration in rat. Mater Sci Eng C 2019;94:190–9. https://doi.org/10.1016/j.msec.2018.09.021 .10.1016/j.msec.2018.09.021 ↗
52. Fomby P, Cherlin AJ, Hadjizadeh A, Doillon CJ, Sueblinvong V, Weiss DJ, Bates JHT, Gilbert T, Liles WC, Lutzko C, Rajagopal J, Prockop DJ, Chambers D, Giangreco A, Keating A, Kotton D, Lelkes PI, Wagner DE, Prockop DJ. Stem cells and cell therapies in lung biology and diseases: Conference report. Ann Am Thorac Soc 2010;12:181–204. https://doi.org/10.1002/term .10.1002/term ↗
53. Huang ZB, Yin GF, Liao XM, Gu JW. Conducting polypyrrole in tissue engineering applications. Front Mater Sci 2014;8:39–45. https://doi.org/10.1007/s11706-014-0238-8 .10.1007/s11706-014-0238-8 ↗
54. Sun B, Long YZ, Zhang HD, Li MM, Duvail JL, Jiang XY, Yin HL. Advances in three-dimensional nanofibrous macrostructures via electrospinning. Prog Polym Sci 2014;39:862–90. https://doi.org/10.1016/j.progpolymsci.2013.06.002 .10.1016/j.progpolymsci.2013.06.002 ↗
55. Blakeney BA, Tambralli A, Anderson JM, Andukuri A, Lim DJ, Dean DR, Jun HW. Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold. Biomaterials 2011;32:1583–90. https://doi.org/10.1016/j.biomaterials.2010.10.056 .10.1016/j.biomaterials.2010.10.056 ↗
56. Wu T, Huang C, Li D, Yin A, Liu W, Wang J, Chen J, Ei-Hamshary H, Al-Deyab SS, Mo X. A multi-layered vascular scaffold with symmetrical structure by bi-directional gradient electrospinning. Colloids Surf B Biointerfaces. 2015;133:179–88. https://doi.org/10.1016/j.colsurfb.2015.05.048.10.1016/j.colsurfb.2015.05.048 ↗
57. Hu Q, Su C, Zeng Z, Zhang H, Feng R, Feng J, Li S. Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells. J Biomater Appl 2020;35:553–66. https://doi.org/10.1177/0885328220935090 .10.1177/0885328220935090 ↗
58. Wu T, Zhang J, Wang Y, Li D, Sun B, El-Hamshary H, Yin M, Mo X. Fabrication and preliminary study of a biomimetic tri-layer tubular graft based on fibers and fiber yarns for vascular tissue engineering. Mater Sci Eng C 2018;82:121–9. https://doi.org/10.1016/j.msec.2017.08.072 .10.1016/j.msec.2017.08.072 ↗
59. Dias JR, Baptista-Silva S, Sousa A, Oliveira AL, Bártolo PJ, Granja PL. Biomechanical performance of hybrid electrospun structures for skin regeneration. Mater Sci Eng C 2018;93:816–27. https://doi.org/10.1016/j.msec.2018.08.050 .10.1016/j.msec.2018.08.050 ↗
60. Shokrollahi M, Bahrami SH, Nazarpak MH, Solouk A. Multilayer nanofibrous patch comprising chamomile loaded carboxyethyl chitosan/poly(vinyl alcohol) and polycaprolactone as a potential wound dressing. Int J Biol Macromol 2020;147:547–59. https://doi.org/10.1016/j.ijbiomac.2020.01.067 .10.1016/j.ijbiomac.2020.01.067 ↗
61. Birhanu G, Tanha S, Akbari Javar H, Seyedjafari E, Zandi-Karimi A, Kiani B, Dehkordi. Dexamethasone loaded multi-layer poly-l-lactic acid/pluronic P123 composite electrospun nanofiber scaffolds for bone tissue engineering and drug delivery. Pharm Dev Technol. 2019;24:338–47. https://doi.org/10.1080/10837450.2018.1481429.10.1080/10837450.2018.1481429 ↗
62. Aghajanpoor M, Hashemi-Najafabadi S, Baghaban- Eslaminejad M, Bagheri F, Mohammad Mousavi S, Azam F, Sayyahpour. The effect of increasing the pore size of nanofibrous scaffolds on the osteogenic cell culture using a combination of sacrificial agent electrospinning and ultrasonication. J Biomed Mater Res Part A. 2017;105:1887–99. https://doi.org/10.1002/jbm.a.36052.10.1002/jbm.a.36052 ↗
63. Türker E, Yildiz ÜH, Arslan A, Yildiz. Biomimetic hybrid scaffold consisting of co-electrospun collagen and PLLCL for 3D cell culture. Int J Biol Macromol. 2019;139:1054–62. https://doi.org/10.1016/j.ijbiomac.2019.08.082.10.1016/j.ijbiomac.2019.08.082 ↗
64. Hodge J, Quint C. The improvement of cell infiltration in an electrospun scaffold with multiple synthetic biodegradable polymers using sacrificial PEO microparticles. J Biomed Mater Res Part A 2019;107:1954–64. https://doi.org/10.1002/jbm.a.36706 .10.1002/jbm.a.36706 ↗
65. Kishan AP, Cosgriff-Hernandez EM. Recent advancements in electrospinning design for tissue engineering applications: a review. J Biomed Mater Res Part A 2017;105:2892–905. https://doi.org/10.1002/jbm.a.36124 .10.1002/jbm.a.36124 ↗
66. Chen Z, Ma S, Hu Y, Lv F, Zhang Y. Preparation of Bi-based porous and magnetic electrospun fibers and their photocatalytic properties in weak polar medium. Colloids Surf A Physicochem Eng Asp 2021;610:125718. https://doi.org/10.1016/j.colsurfa.2020.125718 .10.1016/j.colsurfa.2020.125718 ↗
67. Zhou M, Zhou J, Li R, Xie E. Preparation of aligned ultra-long and diameter-controlled silicon oxide nanotubes by plasma enhanced chemical vapor deposition using electrospun pvp nanofiber template. Nanoscale Res Lett 2010;5:279–85. https://doi.org/10.1007/s11671-009-9476-6 .10.1007/s11671-009-9476-6 ↗
68. Sajedeh K, Atefeh S, Hamid M, Saeedeh M, Jose L, Shahriar MS, Seeram R. A review of key challenges of electrospun scaffolds for tissue-engineering applications. J Tissue Eng Regen Med. 2016;10:715–38. https://doi.org/10.1002/term.10.1002/term ↗
69. Nosar MN, Salehi M, Ghorbani S, Beiranvand SP, Goodarzi A, Azami M. Characterization of wet-electrospun cellulose acetate based 3-dimensional scaffolds for skin tissue engineering applications: influence of cellulose acetate concentration. Cellulose 2016;23:3239–48. https://doi.org/10.1007/s10570-016-1026-7 .10.1007/s10570-016-1026-7 ↗
70. Kishimoto Y, Kobashi T, Morikawa H, Tamada Y. Production of three-dimensional silk fibroin nanofiber non-woven fabric by wet electrospinning. J Silk Sci Technol Japan. 2017;25:49–57. https://doi.org/10.11417/silk.25.49.10.11417/silk.25.49 ↗
71. Wu T, Zhang J, Wang Y, Sun B, Guo X, Morsi Y, El-Hamshary H, El-Newehy M, Mo X. Development of dynamic liquid and conjugated electrospun poly(L-lactide-co-caprolactone)/collagen nanoyarns for regulating vascular smooth muscle cells growth. J Biomed Nanotechnol 2017;13:303–12. https://doi.org/10.1166/jbn.2017.2352 .10.1166/jbn.2017.2352 ↗
72. Sun B, Li J, Liu W, Aqeel BM, El-Hamshary H, Al-Deyab SS, Mo X. Fabrication and characterization of mineralized P(LLA-CL)/SF three-dimensional nanoyarn scaffolds. Iran Polym J (English Ed). 2015;24:29–40. https://doi.org/10.1007/s13726-014-0297-9.10.1007/s13726-014-0297-9 ↗
73. Farzamfar S, Naseri-Nosar M, Vaez A, Esmaeilpour F, Ehterami A, Sahrapeyma H, Samadian H, Hamidieh AA, Ghorbani S, Goodarzi A, Azimi A, Salehi M. Neural tissue regeneration by a gabapentin-loaded cellulose acetate/gelatin wet-electrospun scaffold. Cellulose 2018;25:1229–38. https://doi.org/10.1007/s10570-017-1632-z .10.1007/s10570-017-1632-z ↗
74. Wu J, Hong Y. Enhancing cell infiltration of electrospun fibrous scaffolds in tissue regeneration. Bioact Mater 2016;1:56–64. https://doi.org/10.1016/j.bioactmat.2016.07.001 .10.1016/j.bioactmat.2016.07.001 ↗
75. Wu J, Huang C, Liu W, Yin A, Chen W, He C, Wang H, Liu S, Fan C, Bowlin GL, Mo X. Cell infiltration and vascularization in porous nanoyarn scaffolds prepared by dynamic liquid electrospinning. J Biomed Nanotechnol 2014;10:603–14. https://doi.org/10.1166/jbn.2014.1733 .10.1166/jbn.2014.1733 ↗
76. Nieminen HJ, Laidmäe I, Salmi A, Rauhala T, Paulin T, Heinämäki J, Hæggström E. Ultrasound-enhanced electrospinning. Sci Rep 2018;8:1–6. https://doi.org/10.1038/s41598-018-22124-z .10.1038/s41598-018-22124-z ↗
77. Partheniadis I, Nikolakakis I, Laidmäe I, Heinämäki J. A mini-review: needleless electrospinning of nanofibers for pharmaceutical and biomedical applications. Processes 2020;8:673. https://doi.org/10.3390/PR8060673 .10.3390/PR8060673 ↗
78. Hakkarainen E, Kõrkjas A, Laidmäe I, Lust A, Semjonov K, Kogermann K, Nieminen HJ, Salmi A, Korhonen O, Haeggström E, Heinämäki J. Comparison of traditional and ultrasound-enhanced electrospinning in fabricating nanofibrous drug delivery systems. Pharmaceutics 2019;11:1–10. https://doi.org/10.3390/pharmaceutics11100495 .10.3390/pharmaceutics11100495 ↗
79. Badami AS, Kreke MR, Thompson MS, Riffle JS, Goldstein AS. Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates. Biomaterials 2006;27:596–606. https://doi.org/10.1016/j.biomaterials.2005.05.084 .10.1016/j.biomaterials.2005.05.084 ↗
80. Partheniadis I, Athanasiou K, Laidmäe I, Heinämäki J, Nikolakakis I. Physicomechanical characterization and tablet compression of theophylline nanofibrous mats prepared by conventional and ultrasound enhanced electrospinning. Int J Pharm 2022;616:121558. https://doi.org/10.1016/j.ijpharm.2022.121558 .10.1016/j.ijpharm.2022.121558 ↗
81. Chen Y, Xu W, Shafiq M, Tang J, Hao J, Xie X, Yuan Z, Xiao X, Liu Y, Mo X. Three-dimensional porous gas-foamed electrospun nanofiber scaffold for cartilage regeneration. J Colloid Interface Sci 2021;603:94–109. https://doi.org/10.1016/j.jcis.2021.06.067 .10.1016/j.jcis.2021.06.067 ↗
82. Chen Y, Jia Z, Shafiq M, Xie X, Xiao X, Castro R, Rodrigues J, Wu J, Zhou G, Mo X. Gas foaming of electrospun poly(L-lactide-co-caprolactone)/silk fibroin nanofiber scaffolds to promote cellular infiltration and tissue regeneration. Colloids Surf B Biointerfaces. 2021;201:111637. https://doi.org/10.1016/j.colsurfb.2021.111637.10.1016/j.colsurfb.2021.111637 ↗
83. Pina S, Oliveira JM, Reis RL. Natural-based nanocomposites for bone tissue engineering and regenerative medicine: A review. Adv Mater 2015;27:1143–69. https://doi.org/10.1002/adma.201403354 .10.1002/adma.201403354 ↗
84. Lin W, Chen M, Qu T, Li J, Man Y. Three-dimensional electrospun nanofibrous scaffolds for bone tissue engineering. J Biomed Mater Res Part B Appl Biomater. 2020;108:1311–21. https://doi.org/10.1002/jbm.b.34479.10.1002/jbm.b.34479 ↗
85. Joshi MK, Pant HR, Tiwari AP, Kim HJ, Park CH, Kim CS. Multi-layered macroporous three-dimensional nanofibrous scaffold via a novel gas foaming technique. Chem Eng J. 2015;275:79–88. https://doi.org/10.1016/j.cej.2015.03.121.10.1016/j.cej.2015.03.121 ↗
86. Jiang J, Carlson MA, Teusink MJ, Wang H, MacEwan MR, Xie J. Expanding two-dimensional electrospun nanofiber membranes in the third dimension by a modified gas-foaming technique. ACS Biomater Sci Eng 2015;1:991–1001. https://doi.org/10.1021/acsbiomaterials.5b00238 .10.1021/acsbiomaterials.5b00238 ↗
87. Jiang J, Li Z, Wang H, Wang Y, Carlson MA, Teusink MJ, MacEwan MR, Gu L, Xie J. Expanded 3D nanofiber scaffolds: cell penetration, neovascularization, and host response. Adv Healthc Mater 2016;5:2993–3003. https://doi.org/10.1002/adhm.201600808 .10.1002/adhm.201600808 ↗
88. Gao Q, Gu H, Zhao P, Zhang C, Cao M, Fu J, He Y. Fabrication of electrospun nanofibrous scaffolds with 3D controllable geometric shapes. Mater Des 2018;157:159–69. https://doi.org/10.1016/j.matdes.2018.07.042 .10.1016/j.matdes.2018.07.042 ↗
89. Jing X, Li H, Mi HY, Liu YJ, Tan YM. Fabrication of fluffy shish-kebab structured nanofibers by electrospinning, CO2 escaping foaming and controlled crystallization for biomimetic tissue engineering scaffolds. Chem Eng J 2019;372:785–95. https://doi.org/10.1016/j.cej.2019.04.194 .10.1016/j.cej.2019.04.194 ↗
90. Jiang J, Chen S, Wang H, Carlson MA, Gombart AF, Xie J. CO2-expanded nanofiber scaffolds maintain activity of encapsulated bioactive materials and promote cellular infiltration and positive host response. Acta Biomater 2018;68:237–48. https://doi.org/10.1016/j.actbio.2017.12.018 .10.1016/j.actbio.2017.12.018 ↗
91. Zhang K, Bai X, Yuan Z, Cao X, Jiao X, Li Y, Qin Y, Wen Y, Zhang X. Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing. Biomaterials 2019;204:70–9. https://doi.org/10.1016/j.biomaterials.2019.03.008 .10.1016/j.biomaterials.2019.03.008 ↗
92. Rao F, Yuan Z, Li M, Yu F, Fang X, Jiang B, Wen Y, Zhang P. Expanded 3D nanofibre sponge scaffolds by gas-foaming technique enhance peripheral nerve regeneration. Artif Cells, Nanomedicine, Biotechnol 2019;47:491–500. https://doi.org/10.1080/21691401.2018.1557669 .10.1080/21691401.2018.1557669 ↗
93. Chen Y, Xu W, Shafiq M, Song D, Xie X, Yuan Z, El-Newehy M, El-Hamshary H, Morsi Y, Liu Y, Mo X. Chondroitin sulfate cross-linked three-dimensional tailored electrospun scaffolds for cartilage regeneration. Mater Sci Eng C. 2022. https://doi.org/10.1016/j.msec.2022.112643.10.1016/j.msec.2022.112643 ↗
94. Kim SE, Tiwari AP. Three dimensional polycaprolactone/cellulose scaffold containing calcium-based particles: a new platform for bone regeneration. Carbohydr Polym 2020;250:116880. https://doi.org/10.1016/j.carbpol.2020.116880 .10.1016/j.carbpol.2020.116880 ↗
95. Lee JB, Jeong SI, Bae MS, Yang DH, Heo DN, Kim CH, Alsberg E, Kwon IK. Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration. Tissue Eng Part A 2011;17:2695–702. https://doi.org/10.1089/ten.tea.2010.0709 .10.1089/ten.tea.2010.0709 ↗
96. Jeong SI, Burns NA, Bonino CA, Kwon IK, Khan SA, Alsberg E. Improved cell infiltration of highly porous 3D nanofibrous scaffolds formed by combined fiber-fiber charge repulsions and ultra-sonication. J Mater Chem B 2014;2:8116–22. https://doi.org/10.1039/c4tb01487a .10.1039/c4tb01487a ↗
97. Rahmani A, Hashemi-Najafabadi S, Eslaminejad MB, Bagheri F, Sayahpour FA. The effect of modified electrospun PCL-nHA-nZnO scaffolds on osteogenesis and angiogenesis. J Biomed Mater Res Part A 2019;107:2040–52. https://doi.org/10.1002/jbm.a.36717 .10.1002/jbm.a.36717 ↗
98. Ahtzaz S, Nasir M, Shahzadi L, Iqbal F, Chaudhry AA, Yar M, Ur Rehman I, Amir W, Anjum A, Arshad R. A study on the effect of zinc oxide and zinc peroxide nanoparticles to enhance angiogenesis-pro-angiogenic grafts for tissue regeneration applications. Mater Des. 2017;132:409–18. https://doi.org/10.1016/j.matdes.2017.07.023.10.1016/j.matdes.2017.07.023 ↗
99. Laurenti M, Cauda V. ZnO nanostructures for tissue engineering applications. Nanomaterials 2017;7:374. https://doi.org/10.3390/nano7110374 .10.3390/nano7110374 ↗
100. Chen W, Ma J, Zhu L, Morsi Y, Ei-hamshary H, Al-deyab SS, Mo X. Superelastic, superabsorbent and 3D nanofiber-assembled scaffold for tissue engineering. Colloids Surf B Biointerfaces 2016;142:165–72. https://doi.org/10.1016/j.colsurfb.2016.02.050 .10.1016/j.colsurfb.2016.02.050 ↗